Design of FRET-based GFP probes for detection of protease inhibitors

Biochemical and Biophysical Research Communications

Volumn 323, Issue 2, 2004, Pages 674-678

  Zhang, Bin ^a  

^a ZHEJIANG UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

FRET; GFP; Protease inhibitor; Thrombin

Indexed keywords

GREEN FLUORESCENT PROTEIN; HIRUDIN; HYBRID PROTEIN; PROTEINASE INHIBITOR; THROMBIN; THROMBIN INHIBITOR;

AMINO ACID SEQUENCE; ARTICLE; CONCENTRATION RESPONSE; DRUG DETERMINATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH THROUGHPUT SCREENING; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN DEGRADATION; SEQUENCE ANALYSIS;

ENZYME ACTIVATION; ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HIRUDINS; LUMINESCENT PROTEINS; MOLECULAR PROBES; PROTEASE INHIBITORS; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; THROMBIN;

EID: 4544378666     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.08.143     Document Type: Article

References (19)

1. P. Wu, and L. Brand Resonance energy transfer: methods and applications Anal. Biochem. 218 1994 1 13
2. J.R. Lakowicz Principles of Fluorescence Spectroscopy second ed. 1999 Plenum Press New York
3. L. Stryer Fluorescence energy transfer as a spectroscopic ruler Annu. Rev. Biochem. 47 1978 819 846
4. R.Y. Tsien The green fluorescent protein Annu. Rev. Biochem. 67 1998 509 544
5. R. Heim Green fluorescence protein forms for energy transfer Methods Enzymol. 302 1999 408 423
6. R. Heim, D.C. Prasher, and R.Y. Tsien Wavelength mutations and posttranslational autoxidation of green fluorescent protein Proc. Natl. Acad. Sci. USA 91 1994 12501 12504
7. A.B. Cubitt, R. Heim, S.R. Adams, A.E. Boyd, L.A. Gross, and R.Y. Tsien Understanding, improving and using green fluorescent proteins Trends Biochem. Sci. 20 1995 448 455
8. R. Heim, and R.Y. Tsien Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer Curr. Biol. 6 1996 178 182
9. 2+ based on green fluorescent proteins and calmodulin Nature 388 1997 882 887
10. M.V. Matz, A.F. Fradkov, Y.A. Labas, A.P. Savitsky, A.G. Zaraisky, M.L. Markelov, and S.A. Lukyanov Fluorescent proteins from nonbioluminescent Anthozoa species Nat. Biotechnol. 17 1999 969 973
11. H. Mizuno, A. Sawano, P. Eli, H. Hama, and A. Miyawaki Red fluorescent protein from Discosoma as a fusion tag and a partner for fluorescence resonance energy transfer Biochemistry 40 2001 2502 2510
12. H. Neurath Proteolytic enzymes, past and future Proc. Natl. Acad. Sci. USA 96 1999 10962 10963
13. K. Truong, and M. Ikura The use of FRET imaging microscopy to detect proteinâ€"protein interactions and protein conformational changes in vivo Curr. Opin. Struct. Biol. 11 2001 573 578
14. R.D. Mitra, C.M. Silva, and D.C. Youvan Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein Gene 173 1996 13 17
15. M.a.T. Morgan A, Measurement of caspase activity in individual cells reals differences in the kinetics of caspase activation between cells Cell Death Differ. 8 2001 38 43
16. P. Tawa, J. Tam, R. Cassady, D.W. Nicholson, and S. Xanthoudakis Quantitative analysis of fluorescent caspase substrate cleavage in intact cells and identification of novel inhibitors of apoptosis Cell Death Differ. 8 2001 30 37
17. K.Q. Luo, V.C. Yu, Y. Pu, and D.C. Chang Application of the fluorescence resonance energy transfer method for studying the dynamics of caspase-3 activation during UV-induced apoptosis in living HeLa cells Biochem. Biophys. Res. Commun. 283 2001 1054 1060
18. T. Kohl, K.G. Heinze, R. Kuhlemann, A. Koltermann, and P. Schwille A protease assay for two-photon crosscorrelation and FRET analysis based solely on fluorescent proteins Proc. Natl. Acad. Sci. USA 99 2002 12161 12166
19. B. Zhang, and Z. Peng Defective folding of mutant p16(INK4) proteins encoded by tumor-derived alleles J. Biol. Chem. 271 1996 28734 28737