Seminested PCR for the detection and analysis of flue-cured tobacco (Nicotiana tabacum) expressing a chitinase transgene

Plant Molecular Biology Reporter

Volumn 20, Issue 4, 2002, Pages

  Shi, Jiao ^a   Naylor, Martin ^b   Edwards, Mary Lou ^b   Han, Jin Feng ^a   Cooper, Ian ^b  

^a Agricultural University   (China)

^b CENTRE FOR ECOLOGY AND HYDROLOGY   (United Kingdom)

Author keywords

Baculovirus; Chitinase; Chitinase transgene; Flue cured tobacco; Genetic modification; Seminested PCR

Indexed keywords

EMBRYOPHYTA; NICOTIANA; NICOTIANA TABACUM; UNIDENTIFIED BACULOVIRUS;

EID: 4544351739     PISSN: 07359640     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02772131     Document Type: Article

References (6)

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2. Hawtin RE, Arnold K, Ayres MD, Zanotto PMDA, Howard SC, Gooday GW, Chappel LH, Kitts PA, and Possee RD (1995) Identification and preliminary characterisation of a chitinase gene in the Autographa californica nuclearpolyhedrosis virus genome. Virology 212: 673-685.
3. Hawtin RE, Zarkowska T, Arnold K, Thomas CJ, Gooday GW, King LA, Kuzio JA, and Possee RD (1997) Liquefaction of Autographa californica nucleopolyhedrovirus-infected insects is dependent on the integrity of virus-encoded chitinase and cathepsin genes. Virology 238: 243-253.
4. Lu BY (1998) Study on infrared curing technology of flue-cured tobacco. Chinese Tobacco Sciences 19: 44-47.
5. Meyer R (1999) Development and application of DNA analytical methods for the detection of GMOs in food. Food Control 10: 391-399.
6. Shi J, Thomas CJ, King LA, Hawes CR, Possee RD, Edwards ML, Pallett D, and Cooper JI (2000). The expression of a baculovirus-derived chitinase gene increased resistance of tobacco cultivars to brown spot (Alternaria alternata). Annals of Appl Biol 136: 1-8.