Redesign of catalytic center of an enzyme: Aspartic to serine proteinase

Biochemical and Biophysical Research Communications

Volumn 323, Issue 3, 2004, Pages 947-953

  Tanaka, Takuji ^a   Yada, Rickey Y ^b  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

^b UNIVERSITY OF GUELPH   (Canada)

Author keywords

Catalytic machinery; Chimera; Molecular modeling; Porcine pepsin; Protein engineering; Rationale redesign; Site directed mutagenesis; Trypsin

Indexed keywords

ASPARTIC PROTEINASE; ENZYME; MUTANT PROTEIN; PEPSIN A; SERINE PROTEINASE; SERINE PROTEINASE INHIBITOR; TRYPSIN;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; COMPUTER MODEL; HYDROLYSIS; KINETICS; PRIORITY JOURNAL; PROTEIN FUNCTION;

AMINO ACID SEQUENCE; ASPARTIC ENDOPEPTIDASES; CATALYSIS; COMPUTER SIMULATION; DRUG DESIGN; ENZYME ACTIVATION; ENZYME INHIBITORS; ENZYME STABILITY; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHENYLMETHYLSULFONYL FLUORIDE; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SERINE ENDOPEPTIDASES; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; TRYPSIN;

SUIDAE;

EID: 4544328646     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.08.191     Document Type: Article

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