Immobilization of cell-adhesive peptides to temperature-responsive surfaces facilitates both serum-free cell adhesion and noninvasive cell harvest

Tissue Engineering

Volumn 10, Issue 7-8, 2004, Pages 1125-1135

  Ebara, Mitsuhiro ^a   Yamato, Masayuki ^b   Aoyagi, Takao ^b   Kikuchi, Akihiko ^b   Sakai, Kiyotaka ^a   Okano, Teruo ^b  

^a WASEDA UNIVERSITY   (Japan)

^b TOKYO WOMEN'S MEDICAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ACRYLAMIDE DERIVATIVE; ACRYLIC ACID COPOLYMER; CARBOXYL GROUP; CELL ADHESIVE PEPTIDE; ISOPROPYLACRYLAMIDE; MONOMER; PEPTIDE; POLY(N ISOPROPYLACRYLAMIDE); POLYMER; POLYSTYRENE; TEMPERATURE RESPONSIVE POLYMER; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BOVINE SPONGIFORM ENCEPHALOPATHY; CATTLE; CELL ADHESION; CELL CULTURE; CELL SPREADING; CELL SURFACE; CONTROLLED STUDY; COST BENEFIT ANALYSIS; CULTURE MEDIUM; ENDOTHELIUM CELL; NONHUMAN; POLYMERIZATION; PRION DISEASE; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; SAFETY; SURFACE PROPERTY; TEMPERATURE DEPENDENCE; TISSUE CULTURE; TISSUE ENGINEERING;

ADSORPTION; ANIMALS; CELL ADHESION; CELL ADHESION MOLECULES; CELL CULTURE TECHNIQUES; CELL MOVEMENT; CELL SEPARATION; CELLS, CULTURED; CELLS, IMMOBILIZED; COATED MATERIALS, BIOCOMPATIBLE; CULTURE MEDIA, SERUM-FREE; ENDOTHELIAL CELLS; MATERIALS TESTING; OLIGOPEPTIDES; SURFACE PROPERTIES; SWINE; TEMPERATURE; TISSUE CULTURE TECHNIQUES;

EID: 4544260236     PISSN: 10763279     EISSN: None     Source Type: Journal    
DOI: 10.1089/ten.2004.10.1125     Document Type: Article

References (19)

1. Yamada, N., Okano, T., Sakai, H., Karikusa, F., Sawasaki, Y., and Sakurai, Y. Thermoresponsive polymeric surfaces: Control of attachment and detachment of cultured cells. Macromol. Chem. Rapid Commun. 11, 571, 1990.
2. Yamato, M., Okuhara, M., Karikusa, F., Kikuchi, A., Sakurai, Y., and Okano, T. Signal transduction and cytoskeletal reorganization are required for cell detachment from cell culture surfaces grafted with a temperature-responsive polymer. J. Biomed. Mater. Res. 44, 44, 1999.
3. Kushida, A., Yamato, M., Konno, C., Kikuchi, A., Sakurai, Y., and Okano, T. Decrease in culture temperature releases monolayer endothelial cell sheets together with deposited fibronectin matrix form temperature-responsive culture surfaces. J. Biomed. Mater. Res. 45, 355, 1999.
4. Hirose, M., Kwon, O. H., Yamato, M., Kikuchi, A., and Okano, T. Creation of designed shape cell sheets that are noninvasively harvested and moved onto another surface. Biomacromolecules 1, 377, 2000.
5. Yamato, M., Utsumi, M., Kushida, A., Konno, C., Kikuchi, A., and Okano, T. Thermoresponsive culture dishes allow the intact harvest of multilayered keratinocyte sheets without dispase by reducing temperature. Tissue Eng. 7, 473, 2001.
6. Kushida, A., Yamato, M., Konno, C., Kikuchi, A., Sakurai, Y., and Okano, T. Temperature-responsive culture dishes allow nonenzymatic harvest of differentiated Madin-arby canine kidney (MDCK). cell sheets. J. Biomed. Mater. Res. 51, 216, 2000.
7. Shimizu, T., Yamato, M., Akutsu, T., Shibata, T., Isoi, Y., Kikuchi, A., Umezu, M., and Okano, T. Electrically communicating hree-dimensional cardiac tissue mimic fabricated by layered cultured cardiomyocyte sheet. J. Biomed. Mater. Res. 60, 110, 2002.
8. Shimizu, T., Yamato, M., Isoi, Y., Akutsu, T., Setomaru, T., Abe, K., Kikuchi, A., Umezu, M., and Okano, T. Fabrication of pulsatile cardiac tissue grafts using a novel 3-dimensional cell sheet manipulation technique and temperature-responsive cell culture surfaces. Circ. Res. 90, e40, 2002.
9. Erstad, B.L. Implications of prion-induced diseases for animal-derived pharmaceutical products. Am. J. Health System Pharm. 59, 254, 2002.
10. Wenz, B., Oesch, B., and Horst, M. Analysis of the risk of transmitting bovine spongiform encephalopathy though bone grafts derived from bovine bone. Biomaterials 22, 1599, 2001.
11. Piershbacher, M.D., and Rouslahti, E. Variants of the cell recognition site of fibronectin that retain attachment-promoting activity. Proc. Natl. Acad. Sci. U.S.A. 81, 5985, 1984.
12. Hynes, R.O. Integrins: Versatility, modulation, and signaling in cell adhesion. Cell 69, 11, 1992.
13. Aoyagi, T., Ebara, M., Sakai, K., Sakurai, Y., and Okano, T. Novel bifunctional polymer with reactivity and temperature sensitivity. J. Biomater. Sci. Polym. Ed. 1, 101, 2000.
14. Ebara, M., Yamato, M., Hirose, M., Aoyagi, T., Sakai, K., and Okano T. Copolymerization of 2-carboxyisopropylacrylamide with N-isopropylacrylamide accelerates cell detachment from grafted surfaces by reducing temperature. Biomacromolecules 4, 344, 2003.
15. 3-mediated fibroblast spreading and 140 nm for focal contact and stress fiber formation. J. Cell Biol. 114, 1089, 1991.
16. Wang, H.-B., Dembo, M., and Wang, Y.-L. Substrate flexibility regulates growth and apoptosis of normal but not transformed cells. Am. J. Physiol. Cell. Physiol. 279, C1345, 2000.
17. Choquet, D., Felsenfeld, D.P., and Sheetz, M.P. Extracellular matrix rigidity causes strengthening of integrin-cytoskeleton linkages. Cell 88, 39, 1997.
18. Wong, J.Y., Velasco, A., Rajagopalan, P., and Pham, Q. Directed movement of vascular smooth muscle cells on gradient-compliant hydrogels. Langmuir 19, 1908, 2003.
19. Yeo, W.-S., Hodneland, C.D., and Mrksich, M. Electroactive monolayer substrates that selectively release adherent cells. Chembiochem 7/8, 590, 2001.