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Volumn 2, Issue 7-8, 2001, Pages 590-593

Electroactive monolayer substrates that selectively release adherent cells

Author keywords

Biosurfaces; Cell adhesion; Dynamic substrates; Electrochemistry; Monolayers

Indexed keywords

ARGINYL-GLYCYL-ASPARTIC ACID; ARGINYLGLYCYLASPARTIC ACID; MEMBRANE PROTEIN; OLIGOPEPTIDE; QUINONE DERIVATIVE; SUBSTRATE ADHESION MOLECULES;

EID: 0035800893     PISSN: 14394227     EISSN: None     Source Type: Journal    
DOI: 10.1002/1439-7633(20010803)2:7/8<590::aid-cbic590>3.3.co;2-4     Document Type: Article
Times cited : (80)

References (24)
  • 10
    • 0034600254 scopus 로고    scopus 로고
    • For an example of a model substrate that can switch off the selective association of proteins with immobilized ligands, see: C. D. Hodneland, M. Mrksich, J. Am. Chem. Soc. 2000, 122, 4235-4236.
    • (2000) J. Am. Chem. Soc. , vol.122 , pp. 4235-4236
    • Hodneland, C.D.1    Mrksich, M.2
  • 11
    • 0347800805 scopus 로고    scopus 로고
    • Chemistry and Biological Applications of Polyethylene Glycol
    • "Chemistry and Biological Applications of Polyethylene Glycol": M. Mrksich, G. M. Whitesides, ACS Symp. Ser. 1997, 680, 361-373.
    • (1997) ACS Symp. Ser. , vol.680 , pp. 361-373
    • Mrksich, M.1    Whitesides, G.M.2
  • 12
    • 21244496988 scopus 로고    scopus 로고
    • note
    • The pattern was formed by applying an ethanolic solution of alkanethiols (corresponding to those shown in Scheme 1 A) for 5 minutes to a gold-coated glass substrate, half of which was blocked with a polydimethylsiloxane mask. Following rinsing, the mask was removed, and the substrate was incubated in an ethanolic solution of the second alkanethiol mixture (corresponding to those shown in Scheme 1 B) for 6 hours.
  • 13
    • 85088887521 scopus 로고    scopus 로고
    • note
    • 1H NMR and mass spectra.
  • 16
    • 85088886666 scopus 로고    scopus 로고
    • note
    • 2 atmosphere.
  • 17
    • 21244483965 scopus 로고    scopus 로고
    • note
    • Electrochemistry was performed in a custom-designed electrochemical cell with the monolayer-coated gold as the working electrode, a Pt wire as the counter electrode, and an Ag wire pseudo-reference electrode. All studies were performed with DMEM cell media containing fetal bovine serum (pH 7.4) as the solvent and electrolyte. DMEM contains many components, but the major electrolyte is NaCl (109 mM).
  • 18
    • 0035342522 scopus 로고    scopus 로고
    • For characterization of the specificity in cell adhesion to monolayers of immobilized RGD, see: B. T. Houseman, M. Mrksich, Biomaterials 2001, 22, 943-955.
    • (2001) Biomaterials , vol.22 , pp. 943-955
    • Houseman, B.T.1    Mrksich, M.2
  • 19
    • 21244463558 scopus 로고    scopus 로고
    • note
    • Electrical potentials greater than -800mV can damage the monolayer and make the substrate susceptible to nonspecific adhesion, thereby preventing detachment of cells when soluble peptide is added.
  • 20
    • 21244500236 scopus 로고    scopus 로고
    • note
    • To show that the electrical potential did not affect normal cell behavior, a potential of -700 mV was applied for 4 minutes to cells adhered to the surface shown in Scheme 1 A. As expected, this short electrical pulse did not affect cell morphology, and cells continued to grow and divide normally.
  • 24
    • 0035857564 scopus 로고    scopus 로고
    • Angew. Chem. Int. Ed. 2001, 40, 1093-1096.
    • (2001) Angew. Chem. Int. Ed. , vol.40 , pp. 1093-1096


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.