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For an example of a model substrate that can switch off the selective association of proteins with immobilized ligands, see: C. D. Hodneland, M. Mrksich, J. Am. Chem. Soc. 2000, 122, 4235-4236.
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21244496988
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note
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The pattern was formed by applying an ethanolic solution of alkanethiols (corresponding to those shown in Scheme 1 A) for 5 minutes to a gold-coated glass substrate, half of which was blocked with a polydimethylsiloxane mask. Following rinsing, the mask was removed, and the substrate was incubated in an ethanolic solution of the second alkanethiol mixture (corresponding to those shown in Scheme 1 B) for 6 hours.
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-
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13
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85088887521
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note
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1H NMR and mass spectra.
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-
-
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14
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0032496959
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For the preparation of this monolayer, see: a) C. Roberts, C. S. Chen, M. Mrksich, V. Martichonok, D. E. Ingber, G. M. Whitesides, J. Am. Chem. Soc. 1998, 120, 6548-6555;
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Roberts, C.1
Chen, C.S.2
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Martichonok, V.4
Ingber, D.E.5
Whitesides, G.M.6
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16
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85088886666
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note
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2 atmosphere.
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-
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17
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21244483965
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note
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Electrochemistry was performed in a custom-designed electrochemical cell with the monolayer-coated gold as the working electrode, a Pt wire as the counter electrode, and an Ag wire pseudo-reference electrode. All studies were performed with DMEM cell media containing fetal bovine serum (pH 7.4) as the solvent and electrolyte. DMEM contains many components, but the major electrolyte is NaCl (109 mM).
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18
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0035342522
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For characterization of the specificity in cell adhesion to monolayers of immobilized RGD, see: B. T. Houseman, M. Mrksich, Biomaterials 2001, 22, 943-955.
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Houseman, B.T.1
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19
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21244463558
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note
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Electrical potentials greater than -800mV can damage the monolayer and make the substrate susceptible to nonspecific adhesion, thereby preventing detachment of cells when soluble peptide is added.
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20
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21244500236
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note
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To show that the electrical potential did not affect normal cell behavior, a potential of -700 mV was applied for 4 minutes to cells adhered to the surface shown in Scheme 1 A. As expected, this short electrical pulse did not affect cell morphology, and cells continued to grow and divide normally.
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21
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0029256680
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