Comparison of PCR methods and culture for the detection of Borrelia spp. in patients with erythema migrans

Clinical Microbiology and Infection

Volumn 14, Issue 7, 2008, Pages 653-658

  Cerar, Tjasa ^a   Ruzic Sabljic E ^a   Glinsek U ^a   Zore, A ^a   Strle, F ^b  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

^b UNIVERSITY MEDICAL CENTRE LJUBLJANA   (Slovenia)

Author keywords

Borrelia spp; Cultivation; Erytheme migrans; Identification; PCR; Skin biopsies

Indexed keywords

FLAGELLIN; SPACER DNA;

ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATION; BORRELIA; BORRELIA AFZELII; BORRELIA BURGDORFERI; BORRELIA GARINII; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; ERYTHEMA CHRONICUM MIGRANS; GENE AMPLIFICATION; GENE TARGETING; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; SENSITIVITY ANALYSIS; SKIN BIOPSY; SPECIES IDENTIFICATION;

BORRELIA; BORRELIA AFZELII; BORRELIA BURGDORFERI GROUP; BORRELIA GARINII;

EID: 45249119536     PISSN: 1198743X     EISSN: 14690691     Source Type: Journal    
DOI: 10.1111/j.1469-0691.2008.02013.x     Document Type: Article

References (30)

1. Wang G, van Dam AP, Schwartz I et al. Molecular typing of Borrelia burgdorferi sensu lato: Taxonomic, epidemiological and clinical implications. Clin Microbiol Rev 1999; 12: 633-653.
2. Steere AC. Lyme disease. N Engl J Med 2001; 345: 115-125.
3. Stanek G, Strle F. Lyme borreliosis. Lancet 2003; 362: 1639-1647.
4. Wilske B. Diagnosis of Lyme borreliosis in Europe. Vector Borne Zoonotic Dis 2003; 3: 215-227.
5. Aguero-Rosenfeld ME, Wang G, Schwartz I et al. Diagnosis of Lyme borreliosis. Clin Microbiol Rev 2005; 18: 484-509.
6. Strle F, Nadelman RB, Cimperman J et al. Comparison of culture-confirmed erythema migrans caused by Borrelia burgdorferi sensu stricto in New York State and by Borrelia afzelii in Slovenia. Ann Intern Med 1999; 130: 32-36.
7. Nowakowski J, Schwartz I, Liveris D et al. Laboratory diagnostic techniques for patients with early Lyme disease associated with erythema migrans: A comparison of different techniques. Clin Infect Dis 2001; 33: 2023-2027.
8. Logar M, Ružić-Sabljić E, Maraspin V et al. Comparison of erythema migrans caused by Borrelia afzelii and Borrelia garinii. Infection 2004; 32: 15-19.
9. Zore A, Ružić-Sabljić E, Maraspin V et al. Sensitivity of culture and polymerase chain reaction for the etiologic diagnosis of erythema migrans. Wien Klin Wochenschr 2002; 224: 606-609.
10. Centers for Disease Control. Lyme disease surveillance - United States, 1989-1990. MMMR 1990; 40: 417-421.
11. Jurca T, Ružić-Sabljić E, Lotrič Furlan S et.al. Comparison of peripheral and central biopsy sites for the isolation of Borrelia burgdorferi sensu lato from erythema migrans lesions. Clin Infect Dis 1998; 27: 636-638.
12. Ružić-Sabljić E, Maraspin V, Lotrič-Furlan S et.al. Characterization of Borrelia burgdorferi sensu lato strains isolated from human material in Slovenia. Wien Klin Wochenschr 2002; 114: 544-550.
13. Krüger WH, Pulz M. Detection of Borrelia burgdorferi in cerebrospinal fluid by the polymerase chain reaction. J Med Microbiol 1991; 35: 98-102.
14. Zore A, Petrovec M, Prosenc K et al. Infections of small mammals with Borrelia burgdorferi sensu lato in Slovenia as determined by polymerase chain reaction (PCR). Wien Klin Wochenschr 1999; 111: 997-999.
15. Postic D, Assous MV, Grimont PAD et al. Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. Int J Syst Bacteriol 1994; 44: 743-752.
16. Greer CE, Peterson SL, Kiviat NB et al. PCR amplification from paraffin-embedded tissues: Effects of fixative and fixation time. Am J Clin Pathol 1991; 95: 117-124.
17. Schmidt BL. PCR in laboratory diagnosis of human Borrelia burgdorferi infections. Clin Microb Rev 1997; 10: 185-201.
18. Belfaiza J, Postic D, Bellenger E, Baranton G, Girons IS. Genomic fingerprinting of Borrelia burgdorferi sensu lato by pulse-field gel electrophoresis. J Clin Microbiol 1993; 31: 2873-2877.
19. Busch U, Hizo-Teufel C, Boehmer R et al. Molecular characterisation of Borrelia burgdorferi sensu lato strains by pulse-field electrophoresis. Electrophoresis 1995; 16: 744-747.
20. Picken RN, Cheng Y, Strle F et al. Molecular characterization of Borrelia burgdorferi sensu lato from Slovenia revealing significant differences between tick and human isolates. Eur J Clin Microbiol Infect Dis 1996; 15: 313-323.
21. Ružić-Sabljić E, Strle F, Cimperman J et al. Characterisation of Borrelia burgdorferi sensu lato strains isolated from patients with skin manifestation of Lyme borreliosis residing in Slovenia. J Med Microbiol 2000; 49: 47-53.
22. Liveris D, Wang G, Girao G et al. Quantative detection of Borrelia burgdorferi in 2-mm skin samples of erythema migrans lesions: correlation of results with clinical and laboratory findings. J Clin Microbiol 2002; 40: 1249-1253.
23. Logar M, Lotrič-Furlam S, Maraspin V et al. Has the presence or absence of Borrelia burgdorferi sensu lato as detected by skin culture any influence on the course of erythema migrans? Wien Klin Wochenschr 1999; 111: 945-950.
24. Ružić-Sabljić E, Lotrič-Furlan S, Maraspin V et al. Comparison of isolation rate of Borrelia burgdorferi sensu lato in MKP and BSK-II medium. Int J Med Microbiol 2006; 296: 267-273.
25. Wormser GP, Bittker S, Cooper D et al. Comparison of yields of blood cultures using serum or plasma from patients with early Lyme disease. J Clin Microbiol 2000; 38: 1648-1650.
26. Picken MM, Picken RN, Han D et al. Single-tube nested polymerase chain reaction assay based on flagellin gene sequences for detection of Borrelia burgdorferi sensu lato. Eur J Clin Microbiol Infect Dis 1996; 6: 489-498.
27. Postic D, Merien F, Perolat P et al. The diagnosis of Lyme borreliosis by gene amplification (PCR). In: Lamassoure M-A, ed., Biological diagnosis of leptospirosis-Lyme borreliosis, 2nd edn. Paris: Institut Pasteur, 2000; 215-221.
28. Brettschneider S, Bruckbauer H, Klugbauer N et.al. Diagnostic value of PCR for detection of Borrelia burgdorferi in skin biopsy and urine samples from patients with skin borreliosis. J Clin Microbiol 1998; 36: 2658-2665.
29. Picken MM, Picken RN, Han D et.al. A two year prospective study to compare culture and polymerase chain reaction amplification for the detection and diagnosis of Lyme borreliosis. Mol Pathol 1997; 50: 186-193.
30. Ružić-Sabljić E, Strle F. Comparison of growth of Borrelia afzelii, B. garinii and B. burgdorferi sensu stricto in MKP and BSK-II medium. Int J Med Microbiol 2004; 294: 407-412.