A study of the regulating gene of femA from methicillin-resistant Staphylococcus aureus clinical isolates

Journal of International Medical Research

Volumn 36, Issue 3, 2008, Pages 420-433

  Li, X ^a   Xiong, Y ^a   Fan, Xinjian ^a   Zhong, Z ^a   Feng, P ^a   Tang, H ^a   Zhou, T ^a  

^a SICHUAN UNIVERSITY   (China)

Author keywords

Electrophoretic mobility shift assay (EMSA); femA; Methicillin resistant Staphylococcus aureus (MRSA); Methicillin sensitive Staphylococcus aureus (MSSA); Real time fluorescent quantitative polymerase chain reaction (RT qPCR); Regulating gene

Indexed keywords

BETA LACTAMASE; BIOTIN; FEMA PROTEIN; METICILLIN; NITROCEFIN; OXACILLIN; PENICILLIN BINDING PROTEIN 2A; PSORALEN; REGULATOR PROTEIN;

AGAR DIFFUSION; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM ISOLATE; BASE PAIRING; CONTROLLED STUDY; ENZYME ANALYSIS; GEL MOBILITY SHIFT ASSAY; GENE AMPLIFICATION; GENE LOCATION; GENE SEQUENCE; GENETIC ANALYSIS; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; STAPHYLOCOCCUS AUREUS;

EID: 45149106620     PISSN: 03000605     EISSN: None     Source Type: Journal    
DOI: 10.1177/147323000803600306     Document Type: Article

References (24)

1. Georgopapadakou NH, Smith SA, Bonner DP: Penicillin-binding proteins in a Staphylococcus aureus strain resistant to specific beta-lactam antibiotics. Antimicrob Agents Chemother 1982; 22: 172-175.
2. Berger-Bächi B, Barberis-Maino L, Strässle A, et al: FemA, a host-mediated factor essential for methicillin resistance in Staphylococcus aureus: molecular cloning and characterization. Mol Gen Genet 1989; 219: 263-269.
3. Ton-That H, Labischinski H, Berger-Bächi B, et al: Anchor structure of staphylococcal surface proteins. III. Role of the femA, femB, and femX factors in anchoring surface proteins to the bacterial cell wall. J Biol Chem 1998, 273: 29143-29149.
4. Chen Z, Fan X, Lü X: Combination PCR of mecA, femA genes for detection of MRSA. Sichuan Do Xue Xue Boo Yi Xue Ban 2003; 34: 663-666 [in Chinese, English abstract].
5. Krishnan PU, Miles K, Shetty N: Detection of methicillin and mupirocin resistance in Staphylococcus aureus isolates using conventional and molecular methods: a descriptive study from a burns unit with high prevalence of MRSA. J Clin Pathol 2002, 55: 745-748.
6. Hackbarth CJ, Chambers HF: blaI and blaR1 regulate β-lactamase and PBP 2a production in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 1993; 37: 1144-1149.
7. Hegde SS, Shrader TE: FemABX family members are novel nonribosomal peptidyltransferases and important pathogen-specific drugs targets. J Biol Chem 2001; 276: 6998-7003.
8. National Committee for Clinical Laboratory Standards: MIC Interpretive Standards for Staphylococcus. Washington: National Committee for Clinical Laboratory Standards, MIC testing Supplemental Tables, January 2004; pp 20-24.
9. Pitkälä A, Salmikivi L, Bredbacka P, et al: Comparison of tests for detection of β-lactamase-producing staphylococci. J Clin Microbiol 2007; 45: 2031-2033.
10. Watson JD: Handbook for DNA Isolation and Purification, part 2.04. Shanghai: Watson Biotechnologies.
11. Goerke C, Campana S, Buyer MG, et al: Direct quantitative transcript analysis of the agr regulation of Staphylococcus aureus during human infection in comparison to the expression profile in vitro. Infect Immun 2000; 68: 1304-1311.
12. Rosato AE, Craig WA, Archer GL: Quantitation of mecA transcription in oxacillin-resistant Staphylococcus aureus clinical isolates. J Bacteriol 2003; 185: 3446-3452.
13. Murakami K, Tomasz A: Involvement of multiple genetic determinants in high-level methicillin resistance in Staphylococcus aureus. J Bacteriol 1989; 171: 874-879.
14. De Lencastre H, Wu SW, Pinho MG, et al: Antibiotic resistance as a stress response: complete sequencing of a large number of chromosomal loci in Staphylococcus aureus strain COL that impact on the expression of resistance to methicillin. Microb Drug Resist 1999; 5: 163-175.
15. Maidhof H, Reinicke B, Blümel P, et al: femA, which encodes a factor essential for expression of methicillin resistance, affects glycine content of peptidoglycan in methicillin-resistant and methicillin-susceptible Staphylococcus aureus strains. J Bacteriol 1991; 173: 3507-3513.
16. Hürlimann-Dalel RL, Ryffel C, Kayser FH, et al: Survey of the methicillin resistance-associated genes mecA, mecR1-mecI, and femA-femB in clinical isolates of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 1992; 36: 2617-2621.
17. Alborn WE Jr, Hoskins J, Flokowitsch JE: Cloning and characterization of femA and femB from Staphylococcus epidermidis and Staphylococcus haemolytica. Program and Abstracts of the 34th Interscience Conference on Antimicrobial Agents and Chemotherapy. Washington DC: American Society for Microbiology, 1994; C59.
18. Strandén AM, Ehlert K, Labischinski H, et al: Cell wall monoglycine cross-bridges and methicillin hypersusceptibility in a femAB null mutant of methicillin-resistant Staphylococcus aureus. J Bacteriol 1997; 179: 9-16.
19. Tschierske M, Mori C, Rohrer S, et al: Identification of three additional femAB-like open reading frames in Staphylococcus aureus. FEMS Microbiol Lett 1999, 171: 97-102.
20. Kobayshi N, Wu H, Kojima K, et al: Detection of mecA, femA, and femB genes in clinical strains of staphylococci using polymerase chain reaction. Epidemiol Infect 1994; 113: 259-266.
21. Alborn WE, Hoskins J, Unal S, et al: Cloning and characterization of femA and femB from Staphylococcus epidermidis. Gene 1996; 180: 177-181.
22. Berger-Bächi B, Kohler M: A novel site on the chromosome of Staphylococcus aureus influencing the level of methicillin resistance. genetic mapping. FEMS Microbiol Lett 1983; 20: 305-309.
23. Heinrich M, Bernhard R, Peter B, et al: femA, which encodes a factor essential for expression of methicillin resistance, affects glycine content of peptidoglycan in methicillin-resistant and methicillin-susceptible Staphylococcus aureus strains. J Bacteriol 1991; 173: 3507-3513.
24. Vannuffel P, Heusterspreute M, Bouyer M, et al: Molecular characterization of femA from Staphylococcus hominis and Staphylococcus saprophyticus, and femA-based discrimination of Staphylococcal species. Res Microbiol 1999; 150: 129-141.