High-yield production of secreted active proteins by the Pseudomonas aeruginosa type III secretion system

Applied and Environmental Microbiology

Volumn 74, Issue 11, 2008, Pages 3601-3604

  Derouazi, M ^a   Toussaint, B ^a   Quenee L ^a   Epaulard, O ^a   Guillaume, M ^a   Marlu, R ^a   Polack, B ^a  

^a LABORATOIRE TIMC IMAG   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BIOMOLECULES; COMPUTER NETWORKS; ESCHERICHIA COLI;

ACTIVE PROTEINS; AMERICAN SOCIETY; INCLUSION BODIES; PROTEIN YIELD; PSEUDOMONAS AERUGINOSA (APRIN); RECOMBINANT PROTEIN PRODUCTIONS; SECRETED PROTEINS; TYPE III SECRETION (T3S); YIELD PRODUCTION;

PROTEINS;

BACTERIAL PROTEIN; RECOMBINANT PROTEIN;

BACTERIUM; PROTEIN; RECOMBINATION; SECRETION;

ARTICLE; BACTERIAL GROWTH; BACTERIAL METABOLISM; BIOACCUMULATION; CELL INCLUSION; CULTURE MEDIUM; ESCHERICHIA COLI; NONHUMAN; PROTEIN SECRETION; PROTEIN SYNTHESIS; PSEUDOMONAS AERUGINOSA; QUANTUM YIELD;

BACTERIAL PROTEINS; MEMBRANE TRANSPORT PROTEINS; PROTEIN TRANSPORT; PSEUDOMONAS AERUGINOSA;

ESCHERICHIA COLI; PSEUDOMONAS AERUGINOSA;

EID: 44949263170     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02576-07     Document Type: Article

References (20)

1. Cornelis, G. R., and F. Van Gijsegem. 2000. Assembly and function of type III secretory systems. Annu. Rev. Microbiol. 54:735-774.
2. Cornelis, G. R. 2006. The type III secretion injectisome. Nat. Rev. Microbiol. 4:811-825.
3. Enderle, P. J., and M. A. Farwell. 1998. Electroporation of freshly plated Escherichia coli and Pseudomonas aeruginosa cells. BioTechniques 25:954-956.
4. Epaulard, O., M. Derouazi, C. Margerit, R. Marlu, D. Filopon, B. Polack, and B. Toussaint. 2008. Optimization of a type three secretion system-based P. aeruginosa live vector for antigen delivery. Clin. Vaccine Immunol. 15:308-313.
5. Epaulard, O., B. Toussaint, L. Quénée, M. Derouazi, N. Bosco, C. Villiers, R. Leberre, B. Guery, D. Filopon, L. Crombez, P. N. Marche, and B. Polack. 2006. Anti-tumor immunotherapy via antigen delivery from a live attenuated genetically engineered Pseudomonas aeruginosa type III secretion system-based vector. Mol. Ther. 14:656-661.
6. Frank, D. W. 1997. The exoenzyme S regulon of Pseudomonas aeruginosa. Mol. Microbiol. 26:621-629.
7. Galan, J. E., and H. Wolf-Watz. 2006. Protein delivery into eukaryotic cells by type III secretion machines. Nature 444:567-573.
8. Jurado, P., V. de Lorenzo, and L. A. Fernandez. 2006. Thioredoxin fusions increase folding of single chain Fv antibodies in the cytoplasm of Escherichia coli: evidence that chaperone activity is the prime effect of thioredoxin. J. Mol. Biol. 357:49-61.
9. Kondo, A., J. Kohda, Y. Endo, T. Shiromizu, Y. Kurokawa, K. Nishihara, H. Yanagi, T. Yura, and H. Fukuda. 2000. Improvement of productivity of active horseradish peroxidase in Escherichia coli by coexpression of Dsb proteins. J. Biosci. Bioeng. 90:600-606.
10. Majander, K., L. Anton, J. Antikainen, H. Lng, M. Brummer, T. K. Korhonen, and B. Westerlund-Wikstrm. 2005. Extracellular secretion of polypeptides using a modified Escherichia coli flagellar secretion apparatus. Nat. Biotechnol. 23:475-481.
11. Mergulhão, F. J., D. K. Summers, and G. A. Monteiro. 2005. Recombinant protein secretion in Escherichia coli. Biotechnol. Adv. 23:177-202.
12. Monchois, V., C. Abergel, J. Sturgis, S. Jeudy, and J. M. Claverie. 2001. Escherichia coli ykfE ORFan gene encodes a potent inhibitor of C-type lysozyme. J. Biol. Chem. 276:18437-18441.
13. phox deficient chronic granulomatous disease. Biochem. Biophys. Res. Commun. 275:854-858.
14. Quénée, L., D. Lamotte, and B. Polack. 2005. Combined sacB-based negative selection and cre-lox antibiotic marker recycling for efficient gene deletion in Pseudomonas aeruginosa. BioTechniques 38:63-67.
15. Shen, D. K., L. Quénée, M. Bonnet, L. Kuhn, M. Derouazi, D. Lamotte, B. Toussaint, and B. Polack. Orf1/SpcS chaperones ExoS for the type three secretion by Pseudomonas aeruginosa. Biomed. Environ. Sci., in press.
16. Stark, M. J. R. 1987. Multicopy expression vectors carrying the lac repressor gene for regulated high-level expression of genes in Escherichia coli. Gene 51:255-267.
17. Sugamata, Y., and T. Shiba. 2005. Improved secretory production of recombinant proteins by random mutagenesis of hlyB, an alpha-hemolysin transporter from Escherichia coli. Appl. Environ. Microbiol. 71:656-662.
18. West, S. E. H., H. P. Schweizer, C. Dall, A. K. Sample, and L. J. Runyen-Janecky. 1994. Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa. Gene 148:81-86.
19. Wilharm, G., S. Dittmann, A. Schmid, and J. Heesemann. 2007. On the role of specific chaperones, the specific ATPase, and the proton motive force in type III secretion. Int. J. Med. Microbiol. 297:27-36.
20. Yasukawa, T., C. Kanei-Ishii, T. Maekawa, J. Fujimoto, T. Yamamoto, and S. Ishii. 1995. Increase of solubility of foreign proteins in Escherichia coli by coproduction of the bacterial thioredoxin. J. Biol. Chem. 270:25328-25331.