Using in ovo electroporation to transfect cells in avian somites

Cold Spring Harbor Protocols

Volumn 3, Issue 1, 2008, Pages

  Chen, Ya Xiong ^a   Krull, Christopher E ^b  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID DNA;

ANIMAL CELL; ARTICLE; BONE CELL; CHICKEN; CONTROLLED STUDY; DNA TRANSFER; ELECTRIC CURRENT; ELECTROPORATION; EMBRYO; EMBRYONIC STEM CELL; GENETIC TRANSFECTION; IN OVO ELECTROPORATION; METHODOLOGY; MORTALITY; MUSCLE CELL; MYOTOME; NONHUMAN; OOCYTE; SOMITE;

AVES; SPONDIAS PURPUREA;

EID: 44949196142     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot4924     Document Type: Article

References (5)

1. Chen, Y., Gutmann, D., Haipek, C., Martinsen, B., Bronner-Fraser, M., and Krull, C.E. 2004. Characterization of chicken NF2/merlin suggests regulatory roles in cell proliferation and migration. Dev. Dynamics 229: 541-554.
2. Hamburger, V. and Hamilton, L. 1951. A series of normal stages in the development of the chick embryo. J. Morphol. 88: 49-92.
3. Kadison, S.R. and Krull, C.E. 2008. Transfecting neural crest cells in avian embryos using in ovo electroporation. CSH Protocols doi:10.1101/pdb.prot4925.
4. Linn, S.A. and Krull, C.E. 2008. Transfecting avian motor neurons and their axons using in ovo electroporation. CSH Protocols doi:10.1101/pdb.prot4926.
5. Sahin, M., Greer, P.L., Lin, M.Z., Poucher, H., Eberhart, J., Schmidt, S., Wright, T.M., Shamah, S.M., O'Connell, S., Cowan, C.W., et all 2005. Eph-dependent tyrosine phosphorylation of ephexin1 modulates growth cone collapse. Neuron 46: 191-204.