Chemoenzymatic synthesis of 4-diphosphocytidyl-2-C-methyl-d-erythritol: a substrate for IspE

Tetrahedron Letters

Volumn 49, Issue 29-30, 2008, Pages 4461-4463

  Narayanasamy, Prabagaran ^a   Eoh, Hyungjin ^a   Crick, Dean C ^a  

^a Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

1,2 O ISOPROPYLIDENE ALPHA DEXTRO XYLOFURANOSE; 2 C METHYL DEXTRO ERYTHRITOL 4 PHOSPHATE; 4 DIPHOSPHOCYTIDYL 2 C METHYL DEXTRO ERYTHRITOL; 4 DIPHOSPHOCYTIDYL 2 C METHYL DEXTRO ERYTHRITOL SYNTHASE; ISOPENTENYL DIPHOSPHATE;

ARTICLE; BENZYLATION; ENANTIOMER; ENZYME SYNTHESIS; SCREENING;

EID: 44749086415     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tetlet.2008.05.074     Document Type: Article

References (22)

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21. Reaction mixtures contained 50 mM Tris-HCl (pH 7.4), 200 μM 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG), 1 mM DTT, 100 μM 2-C-methyl-d-erythritol 4-phosphate (MEP), 100 μM CTP, 0.03 U inorganic pyrophosphatase, 1U purine nucleoside phosphorylase and 11.6 pmols of purified M. tuberculosis IspD per 50 μl of reaction volume. Reaction mixtures were incubated at room temperature for 30 min after which their endpoint absorbance at 360 nm was determined. Chemically produced 1 was compared with authentic 1. Synthesized 2 was found to generate spectra identical to reported data.
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