Quantitative analysis of transforming growth factor β isoforms mRNA in the human corneal epithelium

Folia Biologica

Volumn 54, Issue 2, 2008, Pages 46-52

  Strzalka, Barbara ^a   Dorecka, M ^a   Stanik Walentek, A ^a   Kowalczyk, M ^a   Kapral, M ^a   Romaniuk, W ^a   Mazurek, U ^a   Swiatkowska, L ^a  

^a MEDICAL UNIVERSITY OF SILESIA   (Poland)

Author keywords

[No Author keywords available]

Indexed keywords

BETA ACTIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; MESSENGER RNA; POLYACRYLAMIDE GEL; TRANSFORMING GROWTH FACTOR BETA; TRANSFORMING GROWTH FACTOR BETA1; TRANSFORMING GROWTH FACTOR BETA2; TRANSFORMING GROWTH FACTOR BETA3;

ANALYSIS OF VARIANCE; ARTICLE; CONTROLLED STUDY; CORNEA EPITHELIUM; FEMALE; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMAN; HUMAN TISSUE; MALE; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TEMPERATURE;

EID: 44649112016     PISSN: 00155500     EISSN: 25337602     Source Type: Journal    
DOI: None     Document Type: Article

References (37)

1. Border, W. A., Noble, N. A. (1994) Transforming growth factor β in tissue fibrosis. N. Engl. J. Med. 10, 1286-1292.
2. Bustin, S. A. (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25, 169-193.
3. Bustin, S. A. (2002) Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J. Mol. Endocrinol. 29, 23-39.
4. Carrington, L. M., Albon, J., Anderson, I., Kamma, C., Boulton, M. (2006) Differential regulation of key stages in early corneal wound healing by TGF-β isoforms and their inhibitors. Invest. Ophthalmol. Vis. Sci. 47, 1886-1894.
5. Chakravarti, S. (2001) The cornea through the eyes of knockout mice. Exp. Eye Res. 73, 411-419.
6. Chakravarti, S., Wu, F., Vij, N., Roberts, L., Joyce, S. (2004) Microarray studies reveal macrophage-like function of stromal keratocytes in the cornea. Invest. Ophthalmol. Vis. Sci. 45, 3475-3484.
7. Chen, C., Michelini-Norris, B., Stevens, S., Rowsey, J. (2000) Measurement of mRNA for TGFβ and extracellular matrix proteins in corneas of rats after PRK. Invest. Ophthalmol. Vis. Sci. 41, 4108-4116.
8. Chomczynski, P., Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium-thiocyanate phenolchloroform extraction. Anal. Biochem. 162, 156-159.
9. Connor, T. B., Roberts, A. B., Sporn, M. B., Danielpour, D., Dart, L. L., Michels, R. G. (1989) Correlation of fibrosis and transforming growth factor-β type 2 levels in the eye. J. Clin. Invest. 83, 1661-1666.
10. Cordeiro, M. F. (2002) Beyond Mitomycin: TGF-β and wound healing. Prog. Retin. Eye Res. 21, 75-89.
11. Cordeiro, M. F. (2003) Role of transforming growth factor β in conjunctival scarring. Clin. Sci. (Lond.) 104, 181-187.
12. Derynck, R., Jarrett, J. A., Chen, E. Y., Eaton, D. H., Bell, J. R., Assoian, R. K., Roberts, A. B., Sporn, M. B., Goeddel, D. V. (1985) Human transforming growth factor-β complementary DNA sequence and expression in normal and transformed cells. Nature 316, 701-705.
13. Diehn, J. J., Diehn, M., Marmor, M. F., Brown, P. O. (2005) Differential gene expression in anatomical compartments of the human eye. Genome Biol. 6, R 74.
14. Elliott, R. L., Blobe, G. C. (2005) Role of transforming growth factor β in human cancer. J. Clin. Oncol. 23, 2078-2093.
15. Frank, S., Madlener, M., Werner, S. (1996) Transforming growth factors β1, β2, and β3 and their receptors are differentially regulated during normal and impaired wound healing. J. Biol. Chem. 271, 10188-10193.
16. Hales, A. M., Chamberlain, C. G., McAvoy, J. W. (1995) Cataract induction in lenses cultured with transforming growth factor-β. Invest. Ophthalmol. Vis. Sci. 36, 1709-1713.
17. Hayashida-Hibino, S., Watanabe, H. (2001) The effect of TGF-β1 on differential gene expression profiles in human corneal epithelium studied by cDNA expression array. Invest. Ophthalmol. Vis. Sci. 42, 1691-1697.
18. Honma, Y., Nishida, K., Sotozono, C., Kinoshita, S. (1997) Effect of transforming growth factor-β1 and β2 on in vitro rabbit corneal epithelial cell proliferation promoted by epidermal growth factor, keratinocyte growth factor, or hepatocyte growth factor. Exp. Eye Res. 65, 391-396.
19. Jakowlew, S. B., Dillard, P. J., Sporn, M. B., Roberts, A. B. (1988) Complementary deoxyribonucleic acid cloning of an mRNA encoding transforming growth factor-β4 from chicken embryo chondrocytes. Mol. Endocrinol. 2, 1186-1195.
20. Jester, J. V., Petroll, W. M., Cavanagh, H. D. (1999) Corneal stromal wound healing in refractive surgery: the role of myofibroblasts. Prog. Retin. Eye Res. 18, 311-356.
21. Kawakita, T., Espana, E. M., He, H., Hornia, A., Yeh, L. K., Ouyang, J., Liu, C. Y., Tseng, S. C. (2005) Keratocan expression of murine keratocytes is maintained on amniotic membrane by down-regulating transforming growth factor-β signaling. J. Biol. Chem. 280, 27085-27092.
22. Kon, C. H., Occleston, N. L., Aylward, G. W., Khaw, P. T. (1999) Expression of vitreous cytokines in proliferative vitreoretinopathy: a prospective study. Invest. Ophthalmol. Vis. Sci. 40, 705-712.
23. Li, D. Q., Lee, S. B., Tseng, S. C. (1999) Differential expression and regulation of TGF-β1, TGF-β2, TGF-β3, TGF-β RI, TGF-β RII and TGF-β RIII in cultured human corneal, limbal, and conjunctival fibroblasts. Curr. Eye Res. 19, 154-161.
24. Lyon, R. M., Moses, H. L. (1990) Transforming growth factors and regulation of cell proliferation. Eur. J. Biochem. 187, 467-473.
25. Millan, F. A., Denhez, F., Kondaiah, P., Akhurst, R. J. (1991) Embryonic gene expression patterns of TGF β 1, β 2 and β 3 suggest different developmental functions in vivo. Development 111, 131-143.
26. Mita, T., Yamashita, H., Kaji, Y., Obata, H., Yamada, H., Kato, M., Hanyu, A., Suzuki, M., Tobari, I. (1998) Effect of transforming growth factor β on corneal epithelial and stromal cell function in a rat wound healing model after excimer laser keratectomy. Graefes Arch. Clin. Exp. Ophthalmol. 236, 834-843.
27. Nishida, K., Sotozono, C., Adachi, W., Yamamoto, S., Yokoi, N., Kinoshita, S. (1995) Transforming growth factor-β1, β2 and β3 mRNA expression in human cornea. Curr. Eye Res. 14, 235-241.
28. Pasquale, L. R., Dorman-Pease, M. E., Lutty, G. A., Quigley, H. A., Jampel, H. D. (1993) Immunolocalization of TGF-β 1, TGF-β 2, and TGF-β 3 in the anterior segment of the human eye. Invest. Ophthalmol. Vis. Sci. 34, 23-30.
29. Saika, S., Saika, S., Liu, C. Y., Azhar, M., Sanford, L. P., Doetschman, T., Gendron, R. L., Kao, C. W., Kao, W. W. (2001) TGFβ2 in corneal morphogenesis during mouse embryonic development. Dev. Biol. 240, 419-432.
30. Song, Q. H., Klepeis, V. E., Nugent, M. A., Trinkaus-Randall, V. (2002) TGF-β1 regulates TGF-β1 and FGF-2 mRNA expression during fibroblast wound healing. Mol. Pathol. 55, 164-176.
31. Sporn, M. B., Roberts, A. B. (1992) Transforming growth factor-β: recent progress and new challenges. J. Cell. Biol. 119, 1017-1021.
32. Ten Dijke, P. T., Hansen, P., Iwata, K. K., Pieler, C., Foulkes, J. G. (1988) Identification of another member of the transforming growth factor type β gene family. Proc. Natl. Acad. Sci. USA 85, 4715-4719.
33. Thompson, H. G., Mih, J. D., Krasieva, T. B., Tromberg, B. J., George, S. C. (2006) Epithelial-derived TGF-β2 modulates basal and wound-healing subepithelial matrix homeostasis. Am. J. Physiol. Lung Cell Mol. Physiol. 291, L1277-1285.
34. Tricarico, C., Pinzani, P., Bianchi, S., Paglierani, M., Distante, V., Pazzagli, M., Bustin, S. A., Orlando, C (2002) Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal. Biochem. 309, 293-300.
35. Tuli, S. S., Liu, R., Chen, C., Blalock, T. D., Goldsein, M., Schultz, G. S. (2006) Immunohistochemical localization of EGF, TGF-α, TGF-β, and their receptors in rat corneas during healing of excimer laser ablation. Curr. Eye Res. 31, 709-719.
36. Ye, P., Nadkarni, M. A., Hunter, N. (2006) Regulation of E-cadherin and TGF-β3 expression by CD24 in cultured oral epithelial cells Biochem. Biophys. Res. Commun. 349, 229-235.
37. Zieske, J. D., Hutcheon, A. E., Guo, X., Chung, E. H., Joyce, N. C. (2001) TGF-β receptor types I and II are differentially expressed during corneal epithelial wound repair. Invest. Ophthalmol. Vis. Sci. 42, 1465-1471.