Comparison of manual and automatic (MagNA Pure) isolation methods of total RNA from adipose tissue

Molecular Biotechnology

Volumn 38, Issue 3, 2008, Pages 195-201

  Lacinova, Zdenka ^a   Dolinkova, Marketa ^a   Haluzikova, Denisa ^a   Krajickova, Jana ^a   Haluzik, Martin ^a  

^a CHARLES UNIVERSITY   (Czech Republic)

Author keywords

Beta 2 microglobulin; Leptin; RNA extraction; RT PCR

Indexed keywords

BIOCHEMISTRY; MOLECULAR BIOLOGY; ORGANIC ACIDS; POLLUTION DETECTION; POWDERS; RNA; TISSUE;

ADIPOSE TISSUES; AND RECOVERY; BETA-2-MICROGLOBULIN; COMPACT SYSTEMS; GERMANY; LEPTIN; RNA EXTRACTION; RNA ISOLATION; RT-PCR; SURGICAL OPERATIONS;

NUCLEIC ACIDS;

RNA; COMPLEMENTARY DNA; LEPTIN;

ADIPOSE TISSUE; ADULT; ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN TISSUE; INSTRUMENT; INTERMETHOD COMPARISON; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; QUALITATIVE ANALYSIS; QUANTITATIVE ASSAY; RNA EXTRACTION; RNA ISOLATION; RNA PURIFICATION; SUBCUTANEOUS TISSUE; COMPARATIVE STUDY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR BIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ADIPOSE TISSUE; DNA, COMPLEMENTARY; HUMANS; LEPTIN; MOLECULAR BIOLOGY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA;

EID: 44449167753     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-007-9016-5     Document Type: Article

References (14)

1. H. Hauner 2004 The new concept of adipose tissue function Physiology & Behavior 83 653 658
2. A. S. Avram M. M. Avram W. D. James 2005 Subcutaneous fat in normal and diseased states. 1. Introduction Journal of the American Academy of Dermatology 53 663 670
3. A. S. Avram M. M. Avram W. D. James 2005 Subcutaneous fat in normal and diseased states. 2. Anatomy and physiology of white and brown adipose tissue Journal of the American Academy of Dermatology 53 671 683
4. B. B. Kahn J. S. Flier 2000 Obesity and insulin resistance The Journal of clinical investigation 106 473 481
5. V. Glisin R. Crkvenjakov C. Byus 1974 Ribonucleic acid isolated by cesium chloride centrifugation Biochemistry 13 2633 2637
6. P. Chomczynski N. Sacchi 1987 Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction Analytical biochemistry 162 156 159
7. J. Janke S. Engeli K. Gorzelniak A. M. Sharma 2001 Extraction of Total RNA from Adipocytes Hormone and Metabolic Research 33 213 215
8. T. Okamoto S. Okabe 2000 Ultraviolet absorbance at 260 and 280 nm in RNA measurement is dependent on measurement solution International Journal of Molecular Medicine 5 657 659
9. S. A. Bustin 2000 Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays Journal of Molecular Endocrinology 25 169 93
10. R. M. Murphy K. K. Watt D. Cameron-Smith C. J. Gibbons R. J. Snow 2003 Effects of creatine supplementation on housekeeping genes in human skeletal muscle using real-time RT-PCR. Physiol Genomics 15 163 74
11. Y. M. Liu M. Moldes J. P. Bastard E. Bruckert N. Viguerie B. Hainque A. Basdevant D. Langin J. Pairault K. Clement 2004 Adiponutrin: A new gene regulated by energy balance in human adipose tissue The Journal of Clinical Endocrinology and Metabolism 89 2684 2689
12. Y. W. Tang S. E. Sefers H. Li D. J. Kohn G. W. Procop 2005 Comparative evaluation of three commercial systems for nucleic acid extraction from urine specimens Journal of Clinical Microbiology 43 4830 4833
13. K. Tavangar A. R. Hoffman F. B. Kraemer 1990 A micromethod for the isolation of total RNA from adipose tissue Analytical biochemistry 186 60 63
14. I. Louveau S. Chaudhuri T. D. Etherton 1991 An improved method for isolating RNA from porcine adipose tissue Analytical biochemistry 196 309 310