Structural basis for tropomyosin overlap in thin (actin) filaments and the generation of a molecular swivel by troponin-T

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 20, 2008, Pages 7200-7205

  Murakami, Kenji ^a   Stewart, Murray ^b   Nozawa, Kayo ^a   Tomii, Kumiko ^a   Kudou, Norio ^c   Igarashi, Noriyuki ^c   Shirakihara, Yasuo ^d   Wakatsuki, Soichi ^c   Yasunaga, Takuo ^e   Wakabayashi, Takeyuki ^a  

^a TEIKYO UNIVERSITY   (Japan)

^b MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^c INSTITUTE OF MATERIALS STRUCTURE SCIENCE   (Japan)

^d NATIONAL INSTITUTE OF GENETICS   (Japan)

^e KYUSHU INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Calcium; Cardiomyopathy; Troponin

Indexed keywords

ACTIN; CALCIUM; COILED COIL MYOSIN; TROPOMYOSIN; TROPOMYOSIN C; TROPOMYOSIN N; TROPONIN T;

ACTIN FILAMENT; ACTIN MYOSIN INTERACTION; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CRYSTAL STRUCTURE; HEART MUSCLE; NONHUMAN; OPTICAL RESOLUTION; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN STRUCTURE; RABBIT; RECEPTOR AFFINITY; SKELETAL MUSCLE;

ACTINS; ANIMALS; CARDIOMYOPATHIES; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; MODELS, BIOLOGICAL; MODELS, MOLECULAR; MOLECULAR CONFORMATION; MUSCLE, STRIATED; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; RABBITS; TROPOMYOSIN; TROPONIN T; WATER;

EID: 44449160070     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0801950105     Document Type: Article

References (35)

1. Lees-Miller JP, Helfman DM (1991) The molecular basis for tropomyosin isoform diversity. Bioessays 13:429-437.
2. Pollard TD, Borisy GG (2003) Cellular motility driven by assembly and disassembly of actin filaments. Cell 112:453-465.
3. Gunning PW, Schevzov G, Kee AJ, Hardeman EC (2005) Tropomyosin isoforms: Divining rods for actin cytoskeleton function. Trends Cell Biol 15:333-341.
4. Toyoshima C, Wakabayashi T (1985) Three-dimensional image analysis of the complex of thin filaments and myosin molecules from skeletal muscle. V. Assignment of actin in the actin-tropomyosin subfragment-1 complex. J Biochem 97:245-263.
5. McKillop DE, Geeves MA (1993) Regulation of the interaction between actin and myosin subfragment 1: Evidence for three states of the thin filament. Biophys J 65:693-701.
6. 2+-induced switching of troponin and tropomyosin on actin filaments as revealed by electron cryo-microscopy. J Mol Biol 308:241-261.
7. 2+-regulated muscle relaxation at interaction sites of troponin with actin and tropomyosin. J Mol Biol 352:178-201.
8. Stewart M, McLachlan AD (1975) 14 actin binding sites on tropomyosin? Nature 257:331-333.
9. Crick FHC (1957) The packing of α-helices: Simple coiled-coils. Acta Crystallogr 6:689-697.
10. Stewart M (2001) Structural basis for bending tropomyosin around actin in muscle thin filaments. Proc Natl Acad Sci USA 98:8165-8166.
11. McLachlan AD, Stewart M (1976) The 14-fold periodicity in α-tropomyosin and interaction with actin. J Mol Biol 103:271-298.
12. Brown JH, et al (2001) Structure of the mid-region of tropomyosin: Bending and binding sites for actin. Proc Natl Acad Sci USA 98:8496-8501.
13. McLachlan AD, Stewart M (1975) Tropomyosin coiled-coil interactions. J Mol Biol 98:293-304.
14. Moraczewska J, Nicholson-Flynn K, Hitchcock-DeGregori SE (1999) The ends of tropomyosin are major determinants of actin affinity and myosin subfragment 1-induced binding to F-actin in the open state. Biochemistry 38:15885-15892.
15. Ebashi S, Wakabayashi T, Ebashi F (1971) Troponin and its components. J Biochem 69:441-445.
16. White SP, Cohen C, Phillips GN, Jr (1987) Structure of co-crystals of tropomyosin and troponin. Nature 325:826-828.
17. Hitchcock-DeGregori SE, Heald RW (1987) Altered actin and troponin binding of amino-terminal variants of chicken striated muscle alpha-tropomyosin expressed in Escherichia coli. J Biol Chem 262:9730-9735.
18. Monteiro PB, Lataro RC, Ferro JA, Reinach FC (1994) Functional alpha-tropomyosin produced in Escherichia coli. A dipeptide extension can substitute the amino-terminal acetyl group. J Biol Chem 269:10461-10466.
19. Li Y, et al. (2002) The crystal structure of the C-terminal fragment of striated-muscle alpha-tropomyosin reveals a key troponin T recognition site. Proc Natl Acad Sci USA 99:7378-7383.
20. Hinkle A, Tobacman LS (2003) Folding and function of the troponin tail domain. Effects of cardiomyopathic troponin T mutations. J Biol Chem 278:506-513.
21. Gernert KM, Surles MC, Labean TH, Richardson JS, Richardson DC (1995) The Alacoil: A very tight, antiparallel coiled-coil of helices. Protein Sci 4:2252-2260.
22. Pato MD, Mak AS, Smillie LB (1981) Fragments of rabbit striated muscle alphatropomyosin. I. Preparation and characterization. J Biol Chem 256:593-601.
23. Hammell RL, Hitchcock-DeGregori SE (1996) Mapping the functional domains within the carboxyl terminus of alpha-tropomyosin encoded by the alternatively spliced ninth exon. J Biol Chem 271:4236-4242.
24. Greenfield NJ, et al. (2006) Solution NMR structure of the junction between tropomyosin molecules: Implications for actin binding and regulation. J Mol Biol 364:80-96.
25. Nitanai Y, Minakata S, Maeda K, Oda N, Maeda Y (2007) Crystal structures of tropomyosin: Flexible coiled-coil. Adv Exp Med Biol 592:137-151.
26. Whitby FG, Phillips GN, Jr (2000) Crystal structure of tropomyosin at 7 Angstroms resolution. Proteins 38:49-59.
27. Milligan RA (1996) Protein-protein interactions in the rigor actomyosin complex. Proc Natl Acad Sci USA 93:21-26.
28. Maytum R, Geeves MA, Lehrer SS (2002) A modulatory role for the troponin T tail domain in thin filament regulation. J Biol Chem 277:29774-29780.
29. Brown JH, et al. (2005) Structure of the mid-region of tropomyosin: Bending and binding sites for actin. Proc Natl Acad Sci USA 102:18878-18883.
30. 2+-dependent regulation of striated muscle thin filaments. Biochemistry 45:9550-9558.
31. Storoni LC, McCoy AJ, Read RJ (2004) Likelihood enhanced fast rotation functions. Acta Crystallogr D 60:432-438.
32. Jones TA, Zou JY, Cowan SW, Kjeldgaard M (1991) Improved methods for building protein models in electron density maps and for locating errors in these models. Acta Crystallogr A 47:110-119.
33. Brunger AT, et al. (1998) Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr D 54:905-921.
34. Collaborative Computational Project, Number 4 (1994) The CCP4 suite: Programs for protein crystallography. Acta Crystallogr D 50:760-763.
35. Strelkov SV, Burkhard P (2002) Analysis of α-helical coiled coils with the program TWISTER reveals a structural mechanism for stutter compensation. J Struct Biol 137:54-64.