Expression of vascular endothelial growth factor gene in primary cultured rat hepatocytes

Hepatobiliary and Pancreatic Diseases International

Volumn 3, Issue 3, 2004, Pages 444-447

  Wang, Jin Lin ^a,b   Ming, Jun ^a   Zhou, Xiao Dong ^a   Cheng, Ya Jin ^a   Zhang, Lei ^a   Cheng, Jie Shen ^a  

^a SUN YAT SEN UNIVERSITY   (China)

^b DONGGUAN PEOPLE'S HOSPITAL   (China)

Author keywords

Cell; Cultured; Gene therapy; Hepatocyte transplantation; Transfection; Vascular endothelial growth factor; Yellow fluorescent protein

Indexed keywords

VASCULOTROPIN; YELLOW FLUORESCENT PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; GENE CONSTRUCT; GENE VECTOR; GENETIC TRANSFECTION; INTERNAL RIBOSOME ENTRY SITE; LIVER CELL CULTURE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

ANIMALS; BACTERIAL PROTEINS; BLOTTING, WESTERN; CELLS, CULTURED; GENE EXPRESSION; HEPATOCYTES; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; RATS; RATS, INBRED STRAINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSFECTION; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

EID: 4444343791     PISSN: 14993872     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Borel-Rinkes IH, Bijma AM, Kappers WA, Sinaasappel M, Hoek FJ, Jansen PL, et al. Evidence of metabolic activity of adult and fetal rat hepatocytes transplanted into solid supports. Transplantation 1992;54:210-214.
2. Gospodarowicx D, Abraham JA, Schilling J. Isolation and characterization of a vascular endotheliar stellate cells. Proc Natl Sci USA 1989;86:7311.
3. Esser S, Wolburg K, Wolburg H, Breier G, Kurzchalia T, Risau W. Vascular endothelial growth factor induces endothelial fenestrations in vitro. J cell Biol 1998;140:947-959.
4. Assy N, Spira G, Paizi M, Shenkar L, Kraizer Y, Cohen T, et al. Effect of vascular endothelial growth factor on hepatic regenerative activity following partial hepatectomy in rats. J Hep 1999;30:911-915.
5. Ajioka I, Akaike T, Watanabe Y. Expression of endothelial growth factor promotes colonization, vascularization, and growth of transplanted hepatic tissues in the mouse. Hepatology 1999;29:396-402.
6. Dorafshar AH, Angle N, Bryer-Ash M, Huang D, Farooq MM, Gelabert HA, et al. Vascular endothelial growth factor inhibits mitogen-induced vascular smooth muscle cell proliferation. J Surg Res 2003 114:179-186.
7. Gupta S, Rajvanshi P, Sokhi R, Slehria S, Yam A, Kerr A, et al. Entry and integration of transplanted hepatocytes in rat liver plates occur by disruption of hepatic sinusoidal endothelium. Hepatology 1999,29:509-519.
8. Wang JL, Ming J, Zhou XD, Cheng YJ, Cheng JS. Co-transfection and expression of enhanced yellow fluorescent protein and vascular endothelial growth factor double vector on hepatocyte. Acad J Sun Yat-Sen University Med Sci 2002;23: 254-256.
9. Zhou XD, Tokiwa T, Kano J, Mukaiyama T, Tokiwa T. Isolation and primary culture of adult pig hepatocyte. Methods Cell Sci 1998;19:277.
10. Watanabe Y, Nomoto H, Takezawa R, Miyoshi N, Akaike T. Highly efficient transfection into primary cultured mouse hepatocytes by use of cation-liposome: an application for immunization. J Bio Chem 1994;116:1220.
11. Tada H, Maron DJ, Choi EA, Barsoum J, Lei H, Xie Q, et al. Systemic IFN-beta gene therapy results in long-term survival in mice with established colorectal liver metastases. J Clin Invest 2001;108:83-95.
12. Claffey KP, Browm LF, del Aguilla LF, Tognazzi K, Yeo KT, Manseau EJ, et al. Expression of vascular permeability factor/vascular endothelial growth factor by melanoma cells increases tumor growth, angiogenesis and experimental metastasis. Cancer Res 1996;56:172-181.
13. Thomas KA. Vascular endothelial growth factor, a potent and selective angiogenic agent. J Biol Chem 1996;271:603-606.
14. Senger DR, Galli SJ, Dvorak AM, Perruzzi CA, Harvey VS, Dvorak HF. Tumor cells secrete a vascular permeability factor that promotes accumulation of ascites fluid. Science 1983;219: 983-985.
15. Conn G, Bayne ML, Soderman DD, Kwok PW, Sullivan KA, Palisi TM, et al. Amino acid and cDNA sequences of a vascular endothelial cell mitogen that is homologous to platelet-derived growth factor. Proc Natl Acad Sci USA 1990;87:2628-2632.
16. Wang JL, Zhou XD, Ming J, Zhang L, Xue GQ, Cheng YJ, et al. The effect of vascular endothelial growth factor gene transfection on hepatocyte transplantation. Zhong Hua Gan Zhang Bin Zha Zi 2001;9:175-177.
17. Mahato RI, Henry J, Narang AS, Sabek O, Fraga D, Kotb M, et al. Cationic lipid and polymer-based gene delivery to human pancreatic islets. Mol Ther 2003;7:89-100.
18. Wang HP, Chen XP, Ding L. Hepatitis B x protein activated vascular endothelial growth factor expression through hypoxia inducible factor-1 pathway. Zhong Hua Zhong Liu Za Zhi 2003;25:433-436.
19. Tseng W, Haselton FR, Giorgio TD. Transfection by cationic liposomes using simultaneous single cell measurements of plasmid delivery and transgene expression. J Biol Chem 1997;272: 5641-5647.