Regulated compartmentalization of the putative DEAD-box helicase MDDX28 within the mitochondria in COS-1 cells

Experimental Cell Research

Volumn 299, Issue 2, 2004, Pages 294-302

  Valgardsdottir, Rut ^a   Ottersen, Ole Petter ^a   Prydz, Hans ^a  

^a UNIVERSITY OF OSLO   (Norway)

Author keywords

Cox; cytochrome c oxidase; EGFP; enhanced green fluorescence protein; mitochondrial DNA; Mitochondrial inner membrane; mitochondrial targeting signal; mtDNA; MTS; paraformaldehyde; PBS; PFA; phosphate buffered saline; Punctate structures; Transcription inhibition

Indexed keywords

ETHIDIUM BROMIDE; HELICASE; PEPTIDE DERIVATIVE; POLYPEPTIDE; RNA BINDING PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL COMPARTMENTALIZATION; CELL NUCLEUS; CELL STRAIN COS1; CONTROLLED STUDY; CYTOCHEMISTRY; IMMUNOFLUORESCENCE; IMMUNOGOLD STAINING; LIVER MITOCHONDRION; MITOCHONDRIAL MEMBRANE; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; RAT; UPREGULATION;

EID: 4444341362     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.yexcr.2004.05.019     Document Type: Article

References (31)

1. Fujiki Y., Fowler S., Shio H., Hubbard A.L., Lazarow P.B. Polypeptide and phospholipid composition of the membrane of rat liver peroxisomes: comparison with endoplasmic reticulum and mitochondrial membranes. J. Cell Biol. 93:1982;103-110
2. Barrientos A., Barros M.H., Valnot I., Rötig A., Rustin P., Tzagoloff A. Cytochrome oxidase in health and disease. Gene. 286:2002;53-63
3. Slusher L.B., Gillman E.C., Martin N.C., Hopper A.K. mRNA leader length and initiation codon context determine alternative AUG selection for the yeast gene MOD5. Proc. Natl. Acad. Sci. U. S. A. 88:1991;9789-9793
4. Otterlei M., Haug T., Nagelhus T.A., Slupphaug G., Lindmo T., Krokan H. Nuclear and mitochondrial splice forms of human uracil-DNA glycosylase contain a complex nuclear localization signal and a strong classical mitochondrial signal, respectively. Nucleic Acids Res. 26:1998;4611-4617
5. Willer M., Rainey M., Pullen T., Stirling C.J. The yeast CDC9 gene encodes both a nuclear and a mitochondrial form of DNA ligase I. Curr. Biol. 9:1999;1085-1094
6. Lakshimpathy U., Campbell C. The human DNA ligase III gene encodes nuclear and mitochondrial proteins. Mol. Cell. Biol. 19:1999;3869-3876
7. Brokstad K.A., Kalland K.H., Russel W.C., Matthews D.A. Mitochondria protein p32 can accumulate in the nucleus. Biochem. Biophys. Res. Commun. 281:2001;1161-1169
8. Wang Y., Lyu Y.L., Wang J.C. Dual localization of human topoisomerase IIIα to mitochondria and nucleus. Proc. Natl. Acad. Sci. U. S. A. 99:2002;12114-12119
9. Valgardsdottir R., Brede G., Eide L.G., Frengen E., Prydz H. Cloning and characterization of MDDX28, a putative dead-box helicase with mitochondrial and nuclear localization. J. Biol. Chem. 276:2001;32056-32063
10. Valgardsdottir R., Prydz H. Transport signals and transcription inhibition dependent nuclear localization of the putative DEAD-box helicase MDDX28. J. Biol. Chem. 278:2003;21146-21154
11. Carmo-Fonseca M. The contribution of nuclear compartmentalization to gene regulation. Cell. 108:2002;513-521
12. Partikian A., Olveczky B., Swaminathan R., Li Y., Verkman A.S. Rapid diffusion of green fluorescent protein in the mitochondrial matrix. J. Cell Biol. 140:1998;821-829
13. Williamson D.H., Fennel D.J. Visualization of yeast mitochondrial DNA with the fluorescent stain 'DAPI'. Methods Enzymol. 56:1979;728-733
14. Miyakawa I., Sando N., Kawano S., Nakamura S., Kuroiwa T. Isolation of morphologically intact mitochondrial nucleoids from the yeast, Saccharomyces cerevisiae. J. Cell Sci. 88:1987;431-439
15. Kaufman B.A., Newman S.M., Hallberg R.L., Slaughter C.A., Perlman P.S., Butow R.A. In organello formaldehyde crosslinking of proteins to mtDNA: identification of bifunctional proteins. Proc. Natl. Acad. Sci. U. S. A. 97:2000;7772-7777
16. Capaldi R.A., Aggeler R., Gilkerson R., Hanson G., Knowles M., Marcus A., Margineantu D., Marusich M., Murray J., Oglesbee D., et al. A replicating module as the unit of mitochondrial structure and functioning. Biochim. Biophys. Acta. 1555:2002;192-195
17. Hobbs A.E., Srinivasan M., McCaffery J.M., Jensen R.E. Mmm1p, a mitochondrial outer membrane protein, is connected to mitochondrial DNA (mtDNA) nucleoids and required for mtDNA stability. J. Cell Biol. 152:2001;401-410
18. Van Lookeren Campagne M., Oestreicher A.B., van der Krift T.P., Gispen W.H., Verkleij A.J. Freeze-substitution and Lowicryl HM20 embedding of fixed rat brain: suitability for immunogold ultrastructural localization of neural antigens. J. Histochem. Cytochem. 39:1991;1267-1279
19. Takumi Y., Ramirez-Leon V., Rinvik E., Ottersen O.P. Quantitative immunogold analysis suggests different modes of expression of AMPA and NMDA receptors in hippocampal synapses. Nat. Neurosci. 2:1999;618-624
20. Greenawalt J.W. The isolation of outer and inner mitochondrial membranes. Methods Enzymol. 31:1974;310-323
21. Liu M., Spremulli L. Interaction of mammalian mitochondrial ribosomes with the inner membrane. J. Biol. Chem. 275:2000;29400-29406
22. Brown D.A., London E. Function of lipid rafts in biological membranes. Annu. Rev. Cell Dev. Biol. 14:1998;111-136
23. Fujiki Y., Hubbard A.L., Fowler S., Lazarow P.B. Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum. J. Cell Biol. 93:1982;97-102
24. Rivera-Leon R., Gerbi S.A. Small nucleolar RNAs and rRNA processing. Delocalization of some small nucleolar RNPs after actinomycin D treatment to deplete early pre-rRNAs. Chromosoma. 105:1997;506-514
25. Huang S., Deerinck T.J., Ellisman M.H., Spector D.L. The perinucleolar compartment and transcription. J. Cell Biol. 143:1998;35-47
26. Le Panse S., Masson C., Heliot L., Chassery J.M., Junera H.R., Hernandez-Verdun D. 3-D organization of ribosomal transcription units after DRB inhibition of RNA polymerase II transcription. J. Cell Sci. 112:1999;2145-2154
27. Andersen J.S., Lyon C.E., Fox A.H., Leung A.K.L., Lam Y.W., Steen H., Mann M., Lamond A.I. Direct proteomic analysis of the human nucleolus. Curr. Biol. 12:2002;1-11
28. Caceres J.F., Screaton G.R., Krainer A.R. A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm. Genes Dev. 12:1998;55-66
29. Pinol-Roma S., Dreyfuss G. Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm. Nature. 355:1992;730-732
30. Brede G., Solheim J., Prydz H. PSKH1, a novel splice factor compartment-associated serine kinase. Nucleic Acids Res. 30:2002;5301-5309
31. Aspegren A., Bridge E. Release of snRNP and RNA from transcription sites in adenovirus-infected cells. Exp. Cell Res. 276:2002;273-283