Production of linolenic acid in yeast cells expressing an omega-3 desaturase from tung (Aleurites fordii)

JAOCS, Journal of the American Oil Chemists' Society

Volumn 81, Issue 7, 2004, Pages 647-651

  Dyer, John M ^a   Chapital, Dorselyn C ^a   Kuan, Jui Chang W ^a   Shepherd, Hurley S ^a   Tang, Fuqiang ^a,b   Pepperman, Armand B ^a  

^a SOUTHERN REGIONAL RESEARCH CENTER   (United States)

^b NATL CENTER FOR INFECTIOUS DISEASES   (United States)

Author keywords

Aleurites fordii; Linolenic acid; Omega 3 fatty acid desaturase; Saccharomyces cerevisiae; Tung oil

Indexed keywords

BIOSYNTHESIS; CELLS; CULTIVATION; ENZYMES; FUNCTIONS; OILS AND FATS;

ALEURITES FORDII; LINOLENIC ACID; OMEGA-3 FATTY ACID DESATURASE; SACCHAROMYCES CEREVISIAE; TUNG OIL;

YEAST;

ALEURITES; BRASSICA; BRASSICA NAPUS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; SINAPIS ARVENSIS; VERNICIA FORDII;

EID: 4444323885     PISSN: 0003021X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11746-004-956-x     Document Type: Article

References (18)

1. Sonntag, N.O.V., Composition and Characteristics of Individual Fats and Oils, in Bailey's Industrial Oil and Fat Products, 4th edn., edited by D. Swern, John Wiley & Sons, New York, 1979, Vol. 1, pp. 289-477.
2. Thelen, J.J., and J.B. Ohlrogge, Metabolic Engineering of Fatty Acid Biosynthesis in Plants, Metab. Eng. 4:12-21 (2002).
3. Ohlrogge, J., and J. Browse, Lipid Biosynthesis, Plant Cell 7:957-970 (1995).
4. Dyer, J.M., D.C. Chapital, J.C. Kuan, R.T. Mullen, C. Turner, T.A. McKeon, and A.B. Pepperman, Molecular Analysis of a Bifunctional Fatty Acid Conjugase/Desaturase from Tung. Implications for the Evolution of Plant Fatty Acid Diversity, Plant Physiol 750:2027-2038 (2002).
5. Shanklin, J., and E.B. Caboon, Desaturation and Related Modifications of Fatty Acids, Annu. Rev. Plant Physiol. Plant Mol. Biol. 49:611-641 (1998).
6. Tang, F., J.M. Dyer, A.R. Lax, D.S. Shin, D.C. Chapital, and A.B. Pepperman, Nucleotide Sequence of a cDNA Clone for Omega-3 Fatty Acid Desaturase (Accession No. AF061027) from Aleurites fordii Seeds (PGR99-009), Plant Physiol. 119:364 (1999).
7. Shepherd, H.S., J.M. Dyer, F. Tang, D.S. Shih, and A.B. Pepperman, Nucleotide Sequence of a cDNA Clone of a Plastid-Type Omega-3 Fatty Acid Desaturase (Accession No. AF200717) from Tung (Aleurites fordii) Seeds (PGR00-009), Ibid. 122:292 (2000).
8. Dyer, J.M., and R.T. Mullen, Immunocytological Localization of Two Plant Fatty Acid Desaturases in the Endoplasmic Reticulum, FEBS Lett. 494:44-41 (2001).
9. Gietz, R.D., and R.A. Woods, High-Efficiency Transformation in Yeast, in Molecular Genetics of Yeast: Practical Approaches, edited by J.A. Johnston, Oxford University Press, New York, 1994, pp. 121-134.
10. Bligh, E.G., and W.J. Dyer, A Rapid Method of Total Lipid Extraction and Purification, Can. J. Biochem. Physiol 37:911-917 (1959).
11. Wan, C.Y., and T.A. Wilkins, A Modified Hot Borate Method Significantly Enhances the Yield of High-Quality RNA from Cotton (Gossypium hirsutum L.), Anal. Biochem. 223:7-12 (1994).
12. Shanklin, J., E. Whittle, and B.G. Fox, Eight Histidine Residues Are Catalytically Essential in a Membrane-Associated Iron Enzyme, Stearoyl-CoA Desaturase, and Are Conserved in Alkane Hydroxylase and Xylene Monooxygenase, Biochemistry 35:12787-12794 (1994).
13. Jackson, M.R., T. Nilsson, and P.A. Peterson, Identification of a Consensus Motif for Retention of Transmembrane Proteins in the Endoplasmic Reticulum, EMBO J. 9:3153-3162 (1990).
14. Bossie, M.A., and C.E. Martin, Nutritional Regulation of Yeast Δ-9 Fatty Acid Desaturase Activity, J. Bacteriol. 171:6409-6413 (1989).
15. Reed, D.W., U.A. Schäfer, and P.S. Covello, Characterization of the Brassica napus Extraplastidial Linoleate Desaturase by Expression in Saccharomyces cerevisiae, Plant Physiol. 122:715-720 (2000).
16. Dyer, J.M., D.C. Chapital, J.W. Cary, and A.B. Pepperman, Chilling-Sensitive, Post-transcriptional Regulation of a Plant Fatty Acid Desaturase Expressed in Yeast, Biochem. Biophys. Res. Commun. 282:1019-1025 (2001).
17. Meesapyodsuk, D., D.W. Reed, C.K. Savile, P.H. Buist, S.J. Ambrose, and P.S. Covello, Characterization of the Regiochemistry and Cryptoregiochemistry of a Caenorhabditis elegans Fatty Acid Desaturase (Fat-1) Expressed in Saccharomyces cerevisiae, Biochemistry 39:11948-11954 (2000).
18. Dyer, J.M., D.C. Chapital, J.W. Kuan, R.T. Mullen, and A.B. Pepperman, Metabolic Engineering of Saccharomyces cerevisiae for Production of Novel Lipid Compounds, Appl. Microbiol. Biotechnol. 59:224-230 (2002).