Vectors for expressing T7 epitope- and His6 affinity-tagged fusion proteins in S. cerevisiae

BioTechniques

Volumn 24, Issue 5, 1998, Pages 782-788

  Enomoto, Shinichiro ^a   Chen, Guanghui ^a   Berman, Judith ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE; HISTIDINE; HYBRID PROTEIN; PLASMID DNA; RNA POLYMERASE;

ARTICLE; BACTERIOPHAGE T7; CENTROMERE; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; NONHUMAN; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0031920866     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98245st01     Document Type: Article

References (22)

1. Botting, C.H. and R.E. Randall. 1995. Reporter enzyme-nitrilotriacetic acid-nickel conjugates: reagents for detecting histidine-tagged proteins. BioTechniques 19:362-363.
2. Bubeck, P., M. Winkler and W. Bautsch. 1993. Rapid cloning by homologous recombination in vivo. Nucleic Acids Res. 21:3601-3602.
3. Carlson, D., R. Skopp and C. Price. 1997. DNA binding properties of the replication telomere protein. Biochemistry 36:15900-15908.
4. Elble, R. 1992. A simple and efficient procedure for transformation of yeasts. BioTechniques 13:18-20.
5. Enomoto, S., M.S. Longtine and J. Berman. 1994. Enhancement of telomere-plasmid segregation by the X-telomere associated sequence in Saccharomyces cerevisiae involves SIR2, SIR3, SIR4 and ABF1. Genetics 136:757-767.
6. Enomoto, S., P.D. McCune-Zierath, M. Gerami-Nejad and J. Berman. 1997. RLF2, a subunit of yeast chromatin assembly factor-I, is required for telomeric chromatin function in vivo. Genes and Dev. 11:358-370.
7. Feaver, W.J., J.Q. Svejstrup, L. Bardwell, A.J. Bardwell, S. Buratowski, K.D. Gulyas, T.F. Donahue, E.C. Friedberg and R.D. Kornberg. 1993. Dual roles of a multiprotein complex from S. cerevisiae in transcription and DNA repair. Cell 75:1379-1387.
8. Flick, J.S. and J. Thorner. 1993. Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevistae. Mol. Cell Biol. 13:5861-5876.
9. Gietz, R.D. and A. Sugino. 1988. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74:527-534.
10. Hochuli, E., H. Dobeli and A. Schacher. 1987. New metal chelate adsorbent selective for proteins and peptides containing neighboring histidine residues. J. Chromatogr. 411:177-184.
11. Inoue, H., H. Nojima and H. Okayama. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96:23-28.
12. Johnston, M. 1987. A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae. Microbiol. Rev. 51:458-476.
13. Johnston, M. and R. Davis. 1984. Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol. Cell. Biol. 4:1440-1448.
14. Johnston, M., J.S. Flick and T. Pexton. 1994. Multiple mechanisms provide rapid and stringent glucose repression of GAL gene ex pression in Saccharomyces cerevisiae. Mol. Cell. Biol. 14:3834-3841.
15. Kaufman, P.D., R. Kobayashi and B. Stillman. 1997. Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I. Genes and Dev. 11:345-357.
16. Kroll, D.J., H.A.-m. Abdel-Hafiz, T. Marcell, S. Simpson, C.-Y. Chen, A. Autierrez-Hartmann, J.W. Lustbader and J.P. Hoeffler. 1993. A multifunctional prokaryotic protein expression system: overproduction, affinity purification and selective detection. DNA Cell Biol. 12:441-453.
17. Kuriyama, R., M. Kofron, R. Essner, T. Kalo, S. Dragas-Granoic, C.K. Omoto and A. Knodjakov. 1995. Characterization of a minus end-directed kinesin-like motor protein from cultured mammalian cells. J. Cell Biol. 129:1049-1059.
18. Louvion, J.-F., B. Hauvaux-Copf and D. Picard. 1993. Fusion of GAL4-VP16 to a steroid-binding domain provides a tool for gratuitous induction of galactose-responsive genes in yeast. Gene 131:129-134.
19. Ma, H., S. Kunes, P.J. Schatz and D. Botstein. 1987. Plasmid construction by homologous recombination in yeast. Gene 55:201-216.
20. Robzyk, K. and Y. Kassir. 1992. A simple and highly efficient procedure for rescuing autonomous plasmids from yeast. Nucleic Acids Res. 20:3790.
21. Sikorski, R.S. and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19-27.
22. Studier, F.W., A.H. Rosenberg, J.J. Dunn and J.W. Dubendorff. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89.