Validation and comparative analysis of a multiplexed assay for the simultaneous quantitative measurement of Th1/Th2 cytokines in human serum and human peripheral blood mononuclear cell culture supernatants

Journal of Immunological Methods

Volumn 291, Issue 1-2, 2004, Pages 27-38

  Prabhakar, Uma ^a   Eirikis, Edward ^a   Reddy, Manjula ^a   Silvestro, Eva ^a   Spitz, Susan ^a   Pendley II, Charles ^a   Davis, Hugh M ^a   Miller, Bruce E ^a  

^a CENTOCOR INC   (United States)

Author keywords

coefficient of variation; Culture supernatant; CV; IFN ; IL; interferon gamma; interleukin; limit of detection; limit of quantification; LOD; LOQ; Multiplexed; PBMCs; Serum; Th1 Th2 cytokines; TNF ; tumor necrosis factor alpha

Indexed keywords

CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 10; INTERLEUKIN 2; INTERLEUKIN 4; INTERLEUKIN 5; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; BLOOD ANALYSIS; CELL CULTURE; COMPARATIVE STUDY; CONTROLLED STUDY; DATA ANALYSIS; HUMAN; HUMAN CELL; NORMAL HUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; SUPERNATANT; TH1 CELL; TH2 CELL; VALIDATION PROCESS;

CELLS, CULTURED; CULTURE MEDIA, CONDITIONED; CYTOKINES; HUMANS; LEUKOCYTES, MONONUCLEAR; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TH1 CELLS; TH2 CELLS;

EID: 4444304469     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2004.04.018     Document Type: Article

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