Analysis of Cry2A proteins encoded by genes cloned from indigenous isolates of Bacillus thuringiensis for toxicity against Helicoverpa armigera

Current Science

Volumn 86, Issue 4, 2004, Pages 566-570

  Kumar, Shantanu ^a   Udayasuriyan, V ^a   Sangeetha, P ^a   Bharathi, M ^a  

^a TAMIL NADU AGRICULTURAL UNIVERSITY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BACILLUS THURINGIENSIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HELICOVERPA; HELICOVERPA ARMIGERA; HEXAPODA; INSECTA; VISCUM; VISCUM ALBUM;

EID: 4444298278     PISSN: 00113891     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Hofte, H. and Whiteley, H. R., Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev., 1989, 53, 242-255.
2. English, L. and Slatin, S. L., Mode of action of delta-endotoxins from Bacillus thuringiensis: a comparison of other bacterial toxins. Insect Biochem. Mol. Biol., 1992, 22, 1-7.
3. Agrawal, N., Malhotra, P. and Bhatnagar, R. K., Interaction of gene-cloned and insect cell-expressed aminopeptidase N of Spodoptera litura with insecticidal crystal protein Cry1C. Appl. Environ. Microbiol., 2002, 68, 4583-4592.
4. Kranthi, K. R., Kranthi, S., Ali, S. and Banerjee, S. K., Resistance of Cry1Ac δ-endotoxin of Bacillus thuringiensis in a laboratory selected strain of Helicoverpa armigera (Hubner). Curr. Sci., 2000, 78, 1001-1004.
5. Akhurst, R., Games, B. and Bird, L., Proceedings of the 10th Australian Cotton Conference, Brisbane, 2000.
6. Morse, R. J., Yamamoto, T. and Stroud, R. M., Structure of Cry2Aa suggests an unexpected receptor binding epitope. Structure, 2001, 9, 409-417.
7. Perlak, F. J. et al., Development and commercial use of Bollgard® cotton in the USA - early promises versus today's reality. Plant J., 2001, 27, 489-501.
8. Chakrabarti, S. K., Mandaokar, A., Kumar, A. P. and Sharma, R. P., Efficacy of lepidopteran-specific delta-endotoxin of Bacillus thuringiensis against Helicoverpa armigera. J. Invertebr. Pathol., 1998, 72, 336-337.
9. Babu, B. G., Udayasuriyan, V., Asia Mariam, M., Sivakumar, N., Bharathi, M. and Balasubramanian, G., Comparative toxicity of Cry1Ac and Cry2Aa δ-endotoxins of Bacillus thuringiensis against Helicoverpa armigera (H.). Crop Protect., 2002, 21, 817-822.
10. Rajamohan, F., Hussain, S. R. A., Cotrill, J. A., Gould, F. and Dean, H. D., Mutation in domain II, loop 3 of Bacillus thuringiensis cry1Ab and cry1Ab delta endotoxin suggested loop 3 is involved in initial binding of lepidopteran midgets. J. Biol. Chem., 1996, 271, 25220-25225.
11. Udayasuriyan, V., Nakamura, A., Mori, A., Masaki, H. and Uozumi, T., Cloning of a new cry1A(a) gene from Bacillus thuringiensis strain FU-2-7 and analysis of chimeric cry1A(a) proteins of toxicity. Biosci. Biotechnol. Biochem., 1994, 58, 830-835.
12. Shantanu Kumar and Udayasuriyan, V., Cloning of cry2Aa and cry2Ab genes from new isolates of Bacillus thuringiensis and their expression in recombinant Bacillus thuringiensis and Escherichia coli strains. World J. Microbiol. Biotechnol., 2004, 20, 11-17.
13. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227, 680-685.
14. Patel, R. C., Patel, J. K., Patel, P. B. and Singh, R., Mass breeding of Heliothis armigera (H.). Indian J. Entomol., 1968, 30, 272-280.
15. Schien, C. H., Production of soluble recombinant proteins in bacteria. Bio/Technology, 1989, 7, 1141-1148.
16. Widner, W. R. and Whiteley, H. R., Two highly related crystal proteins of Bacillus thuringiensis. J. Bacteriol., 1989, 171, 965-974.
17. Misra, H. S., Khairnar, N. P., Mathur, M., Vijayalakshmi, N., Hire, R. S., Dongre, T. K. and Mahajan, S. K., Cloning and characterization of an insecticidal crystal protein gene from Bacillus thuringiensis subspecies Kenyae. J. Genet., 2002, 81, 5-11.
18. Moar, W. J., Trumble, J. H., Hice, R. H. and Backman, P. A., Insecticidal activity of the CryIIA protein from NRD-12 isolates of Bacillus thuringiensis subsp. kurstaki expressed in Escherichia coli and Bacillus thuringiensis and in a leaf colonizing strain of Bacillus cereus. Appl. Environ. Microbiol., 1994, 60, 896-902.
19. Rudolph, R. and Lilie, H., In vitro folding of inclusion body proteins. FASEB J., 1996, 10, 49-56.
20. Sudha, S. N., Jayakumar, R. and Sekar, V., Introduction and expression of the cry1Ac gene of Bacillus thuringiensis in a cereal-associated bacterium, Bacillus polymyxa. Curr. Microbiol., 1999, 38, 163-167.
21. Tuli, R., Saluja, J. and Notani, N. K., Cloning and expression in Escherichia coli of entomotoxic protein gene from Bacillus thuringiensis subspecies kurstaki. J. Genet., 1989, 68, 147-160.
22. Schnepf, H. E. and Whiteley, H. R., Cloning and expression of the Bacillus thuringiensis protein gene in Escherichia coli. Proc. Natl. Acad. Sci. USA, 1981, 78, 2989-2897.
23. Bosch, D., Visser, B. and Stiekema, W. J., Analysis of non-active engineered Bacillus thuringiensis crystal proteins. FEMS Microbiol. Lett., 1994, 118, 129-134.
24. Dankocsik, C., Donovan, W. P. and Jany, C. S., Activation of a cryptic protein gene of Bacillus thuringiensis subspecies kurstaki by gene fusion and determination of crystal protein insecticidal specificity. Mol. Microbiol., 1990, 4, 2087-2094.