Cloning vectors for expression-PCR products

BioTechniques

Volumn 37, Issue 3, 2004, Pages 346-347

  Betton, Jean Michel ^a,b  

^a INSTITUT PASTEUR   (France)

^b CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID DNA; RECOMBINANT PROTEIN;

ARTICLE; CLONING; CLONING VECTOR; DNA TEMPLATE; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN SYNTHESIS;

ESCHERICHIA COLI; UNIDENTIFIED CLONING VECTOR; VECTORS;

EID: 4444295162     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/04373bm01     Document Type: Article

References (12)

1. Betton, J.-M. 2003. Rapid Translation System (RTS): a promising alternative for recombinant protein production. Curr. Protein Pept. Sci. 4:73-80.
2. Kain, K.C., P.A. Orlandi, and D.E. Lanar. 1991. Universal promoter for gene expression without cloning: expression-PCR. BioTechniques 10:366-374.
3. Spirin, A.S., V.I. Baranov, L.A. Ryabova, S. Odovov, and Y.B. Alakhov. 1988. A continuous cell-free translation system capable of producing polypeptides in high yield. Science 242:1162-1164.
4. Bernard, P., P. Gabant, E.M. Bahassi, and M. Couturier. 1994. Positive-selection vectors using the F plasmid ccdB killer gene. Gene 148:71-74.
5. Liu, Z.-G. and L.M. Schwartz. 1992. An efficient method for blunt-end ligation of PCR products. BioTechniques 12:28-30.
6. Weiner, M.P. 1993. Directional cloning of blunt-ended PCR products. BioTechniques 15:502-505.
7. Simcox, T.G., S.J. Marsh, E.-A. Gross, W. Lernhardt, S. Davis, and M.-E.C. Simcox. 1991. SrfI, a new type-II restriction endonuclease that recognizes the octanucleotide sequence, 5′-GCCCGGGC-3′. Gene 109:121-123.
8. Loris, R., M.-H. Dao-Thi, E.M. Bahassi, L. Van Melderen, F. Poortmans, R. Liddington, M. Couturier, and L. Wyns. 1999. Crystal structure of CcdB, a topoisomerase poison from E. coli. J. Mol. Biol. 285:1667-1677.
9. Bartolomé, B., Y. Jubete, E. Martinez, and F. de la Cruz. 1991. Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives. Gene 102:75-78.
10. Grandori, R., K. Struck, K. Giovanielli, and J. Carey. 1997. A three-step PCR protocol for construction of chimeric proteins. Protein Eng. 10:1099-1100.
11. Costa, G.L., A. Grafsky, and M.P. Weiner. 1994. Cloning and analysis of PCR-generated DNA fragments. PCR Methods Appl. 3:338-345.
12. Studier, F.W., A.H. Rosenberg, J.J. Dunn, and J.W. Dubendorff. 1990. Use of T7 polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89.