A three-step PCR protocol for construction of chimeric proteins

Protein Engineering

Volumn 10, Issue 9, 1997, Pages 1099-1100

  Grandori, Rita ^a,b   Struck, Kara ^a   Giovanielli, Kira ^a   Carey, Jannette ^a  

^a PRINCETON UNIVERSITY   (United States)

^b UNIVERSITY OF SALZBURG   (Austria)

Author keywords

Chimeric proteins; PCR; Protein structure; Protein subdomains; WrbA

Indexed keywords

CHIMERIC PROTEIN;

ARTICLE; DNA TRANSPOSITION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TECHNIQUE;

EID: 0031450804     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/10.9.1099     Document Type: Article

References (11)

1. Grandori, R. and Carey, J. (1994) Protein Sci., 3, 2185-2193.
2. Higuchi, R., Krummel, B. and Saiki, R.K. (1988) Nucleic Acids Res., 16, 7351-7367.
3. Laemmli, U.K. (1970) Nature, 227, 680-685.
4. Levitt, M. and Chothia, C. (1976) Nature, 261, 552-558.
5. McClelland, M. (1992) Methods Enzymol., 216, 279-303.
6. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
7. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) Proc. Natl Acad. Sci. USA, 74, 5463-5467.
8. Stock, A.M., Mottonen, J.M., Stock, J.B. and Schutt, C.E. (1989a) Nature. 337, 745-749.
9. Stock, J.B., Ninfa, A.J. and Stock, A.M. (1989b) Microbiol. Rev., 53, 450-490.
10. Wu, L., Grandori, R. and Carey, J. (1994) Protein Sci., 3, 369-371.
11. Yang, W., Liuying, N. and Somerville.R.L. (1993) Proc. Natl Acad. Sci. USA, 90, 5796-5800.