Antigenic activity of three chimeric synthetic peptides of the transmembrane (Gp41) and the envelope (Gp120) glycoproteins of HIV-1 virus

Preparative Biochemistry and Biotechnology

Volumn 34, Issue 3, 2004, Pages 227-237

  Marin, Milenen Hernández ^a   Peña, Lilliam Pozo ^a   Tanty, Chryslaine Rodríguez ^b   Clarke, David Higginson ^b   Arenas, Martha Amat ^a   Noguerol, Kenia Ruenes ^a   León, Carlos Silva ^a  

^a Immunoassay Center Cuba   (Cuba)

^b NATL CENTER FOR SCIENTIFIC RESEARCH   (Cuba)

Author keywords

Chimeric synthetic peptides; gp120; gp41; HIV 1; UMELISA

Indexed keywords

GLYCOPROTEIN GP 120; GLYCOPROTEIN GP 41; HUMAN IMMUNODEFICIENCY VIRUS ANTIBODY; PEPTIDE;

ARTICLE; BLOOD; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IMMUNOLOGY;

ENZYME-LINKED IMMUNOSORBENT ASSAY; HIV ANTIBODIES; HIV ENVELOPE PROTEIN GP120; HIV ENVELOPE PROTEIN GP41; HIV INFECTIONS; HIV-1; HUMANS; PEPTIDES;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 2;

EID: 4444219655     PISSN: 10826068     EISSN: None     Source Type: Journal    
DOI: 10.1081/PB-200026804     Document Type: Article

References (20)

1. Sinoussi, F.B.; Chermann, J.C.; Rey, F.; Nugeyre, M.T.; Chamaret, S.; Gruest, J.; Dauguet, C.; Axler-Blin, C.; Vezinet-Brun, F.; Rouzioux, C.; Rezenbaum, W.; Montagnier, L. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immunodeficiency syndrome (AIDS). Science 1983, 220 (4599), 868-871.
2. Gottlieb, M.S.; Groopman, J.E.; Weinstein, W.M.; Fahey, J.L.; Detels, R. The acquired immunodeficiency syndrome. Ann. Intern. Med. 1983, 99 (2), 208-220.
3. Calarota, S.; Jansson, M.; Levi, M.; Broliden, K.; Libonatti, O.; Wigzell, H.; Wahren, B. Immunodominant glycoprotein 41 epitope identified by seroreactivity of HIV type-1 infected individuals. AIDS Res. Hum. Retrovir. 1996, 12 (8), 705-713.
4. Ferrer, M.; Sullivan, B.J.; Godbout, K.L.; Burke, E.; Stump, H.S.; Godoy, J.; Golden, A.; Profy, A.T.; van Schravendijk, M.R. Structural and functional characterization of an epitope in the conserved C-terminal region of HIV-1 gp120. J. Pept. Res. 1999, 54 (1), 32-42.
5. Narvanen, A.; Korkolainen, M.; Suni, J.; Korpela, J.; Kontio, S.; Partanen, P.; Vaheri, A.; Huhtala, M.L. Synthetic env gp41 peptide as a sensitive and specific diagnostic reagent in different stages of human immunodeficiency virus type 1 infection. J. Med. Virol. 1988, 26 (2), 111-118.
6. Shah, K.; Davis, C.; Wilson, J.; Parekh, B. Chimeric synthetic peptides as antigens for detection of antibodies to HIV-1 and HIV-2. East African Med. J. 1996, 73 (1), 63-66.
7. Gokulan, K.; Rao, D.N.; Tripathi, S.P. Advantage of dimeric peptide antigens in serodiagnosis of HIV-1 infection. Microbiol. Immunol. 1997, 41 (3), 215-220.
8. Hernández, M.; Selles, M.E.; Pozo, L.; Gómez, I.; Melchor, A. Antigenicity of Chimeric synthetic peptides based on HTLV-I antigens and impact of epitope orientation. Biochem. Biophys. Res. Commun. 2000, 276 (3), 1085-1088.
9. Hernández, M.; Pozo, L.; Gómez, I.; Melchor, A. Chimeric synthetic peptide as antigen for immunodiagnosis of HIV-1 infection. Biochem. Biophys. Res. Commun. 2000, 272 (1), 259-262.
10. Hernández, M.; Rodríguez, I.; Pozo, L.; Rivero, J. Chimeric synthetic peptides from the nucleocapsid p24 protein of human immunodeficiency virus type-1. Biochem. Biophys. Res. Commun. 2001, 282 (1), 1-3.
11. Hernández, M.; Castellanos, P.; Márquez, Y.; Pozo, L.; Díaz, Y.; González, L.J. Chimeric synthetic peptides containing two immunodominant epitopes from the envelope gp46 and the transmembrane gp21 glycoproteins of HTLV-I virus. Biochem. Biophys. Res. Commun. 2001, 289 (1), 1-6.
12. Hernández, M.; Castellanos, P.; Márquez, Y.; Pozo, L.; Díaz, Y.; González, L.J. Chimeric synthetic peptides from envelope (gp46) and transmembrane (gp21) glycoproteins for the detection of antibodies to Human T-cell Leukemia virus type II. Biochem. Biophys. Res. Commun. 2001, 289 (1), 7-12.
13. Hernández, M.; Márquez, Y.; Vázquez, R.; Rodríguez, Ch.; Higginson, D.; Pozo, P.; Silva, C. Use of a chimeric synthetic peptide from the core p19 protein and the envelope gp46 glycoprotein in the immunodiagnosis of HTLV-II virus infection. Prep. Biochem. & Biotechnol. 2003, 33 (1), 29-38.
14. Merrifield, R.B. J. Solid phase peptide synthesis: The synthesis of a tetrapeptide. Chem. Soc. 1963, 85, 2149-2154.
15. Sarin, V.K.; Tam, J.P.; Merrifield, R.B. Quantitative monitoring of solid-phase peptide synthesis by the ninhydrin reaction. Ann. Biochem. 1981, 117 (1), 147-157.
16. 2 mechanism for the desprotection of synthetic peptides by hydrogen fluoride. Studies to minimize the tyrosine alkylation side reaction. Int. J. Pept. Protein Res. 1983a, 21 (1), 57-60.
17. 2 deprotection of synthetic peptides with a low concentration of HF in dimethyl sulfide: evidence and application in peptide synthesis. J. Am. Chem. Soc. 1983b, 105, 6442-6455.
18. Burdick, D.J.; Stults, J.T. Analysis of peptides synthesis products by electrospray ionization mass spectrometry. Meth. Enzymol. 1997, 289, 499-519.
19. Hopp, T.P.; Woods, K.R. Prediction of protein antigenic determinants from amino acid sequences. Proc. Natl. Acad. Sci. USA. 1981, 78 (6), 3824-3828.
20. Stigler, R.D.; Ruker, F.; Katinger, D.; Elliot, G.; Hohne, W.; Henklein, P. Interaction between a Fab fragment against gp41 of human immunodeficiency virus 1 and its peptide epitope: characterization using a peptide epitope library and molecular modeling. Protein Eng. 1995, 8 (5), 471-479.