Overexpression, physicochemical characterization, and modeling of a hyperthermophilic Pyrococcus furiosus type 2 IPP isomerase

Proteins: Structure, Function and Genetics

Volumn 71, Issue 4, 2008, Pages 1699-1707

  Dutoit, Raphaël ^a   De Ruyck, Jérôme ^b   Durisotti, Virginie ^a   Legrain, Christianne ^a   Jacobs, Eric ^a   Wouters, Johan ^a,b  

^a Inst de Rech Microbiol JM Wiame   (Belgium)

^b UNIVERSITY OF NAMUR   (Belgium)

Author keywords

Flavoprotein; Hyperthermophiles; IDI2; IPP; Mevalonate pathway; Pyrococcus furiosus; Thermostability

Indexed keywords

ISOPENTENYL DIPHOSPHATE DELTA ISOMERASE; MONOMER;

ARTICLE; CONTROLLED STUDY; ENZYME ASSAY; ESCHERICHIA COLI; GENE OVEREXPRESSION; HYPERTHERMOPHILE; MESOPHILE; MOLECULAR CLONING; MOLECULAR MODEL; NONHUMAN; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN PURIFICATION; PYROCOCCUS FURIOSUS; RESIDUE ANALYSIS; STREPTOCOCCUS PNEUMONIAE; TEMPERATURE; THERMOSTABILITY;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CARBON-CARBON DOUBLE BOND ISOMERASES; CLONING, MOLECULAR; CONSERVED SEQUENCE; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; ESCHERICHIA COLI; HEAT; HYDROGEN BONDING; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN FOLDING; PYROCOCCUS FURIOSUS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

PYROCOCCUS FURIOSUS; STREPTOCOCCUS PNEUMONIAE;

EID: 44349175520     PISSN: 08873585     EISSN: 10970134     Source Type: Journal    
DOI: 10.1002/prot.21863     Document Type: Article

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