Amelioration of a Reverse Transcription Polymerase Chain Reaction (RT-PCR) for the detection of ASSVd, PBCVd and PLMVd viroids, and their presence in cultivated and wild pome and stone fruits in Greece

Acta Horticulturae

Volumn 781, Issue , 2008, Pages 519-527

  Boubourakas, I N ^a   Arambatzis, C ^a   Kyriakopoulou, P E ^a   Dovas, C ^b  

^a AGRICULTURAL UNIVERSITY OF ATHENS   (Greece)

^b INSTITUTE OF TECHNOLOGY OF AGRICULTURAL PRODUCTS   (Greece)

Author keywords

Betaine; DMSO; Hot start pcr; mFold; Tetramethylene sulfoxide

Indexed keywords

APPLE SCAR SKIN VIROID; CRATAEGUS; CYDONIA OBLONGA; MALUS X DOMESTICA; PEACH LATENT MOSAIC VIROID; PRUNUS ARMENIACA; PRUNUS PERSICA; PYRUS; VIROIDS;

EID: 44349124670     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/actahortic.2008.781.76     Document Type: Conference Paper

References (19)

1. Barba, M. 1998. Virus certification of fruit tree propagative material in Western Europe. p.288-293. In: A. Hadidi, R.K. Khaterpal and H. Koganezawa (eds.), Plant Virus Disease Control. APS Press, St.Paul, MN, USA.
2. Barba, M., Gumpf, D.J. and Hadidi, A. 2003. Quarantine of imported Germplasm. p.303-311. In: A. Hadidi, R. Flores, J.W. Randles and J.S. Semancik (eds.), Viroids. CSRO Publishing, Collingwood, VIC, Australia.
3. Chakrabarti, R. and Schutt, C.E. 2002. Novel sulfoxides facilitate GC-rich template amplification. BioTechniques 32:866-874.
4. Coffin, R.S. and Coutts R.H.A. 1992. Ds RNA cloning and diagnosis of Beet pseudoyellows virus, by PCR and nucleic acid hybridization. Intervirology 33:197-203.
5. DeStefano, J.J., Buiser, R.G., Mallaber, L.M., Myers, T.W., Bambara, R.A. and Fay, P.J. 1991. Polymerization and RNase-H activities of the reverse transcriptases from Avian-myeloblastosis, Human immunodeficiency, and Moloney murine leukemia viruses are functionally uncoupled. J. Biol. Chem. 266:7423-7431.
6. Hadidi, A., Flores, R., Randles, J.W., and J.S. Semancik.2003. Viroids. 370 pages. CSRO Publishing, Collingwood, VIC, Australia.
7. Hadidi, A. and Yang, X. 1990. Detection of pome fruit viroids by enzymatic cDNA amplification. J. Virol. Methods 30:261-270.
8. Hassen, I.F., Roussel, S., Kummert, J., Fakhfakh, H., Marrakchi, M. and Jijakli, H. 2006. Development of a rapid RT-PCR test for the detection of Peach latent mosaic viroid, Pear blister canker viroid, Hop stunt viroid and Apple scar scin viroid in fruit trees from Tunisia. J. Phytopath. 154(4):217-223.
9. Kyriakopoulou, P.E., Giunchedi, L. and Hadidi, A. 2001. Peach latent mosaic and pome fruit viroids in naturally infected cultivated pear Pyrus communis and wild pear P. amygdaliformis: Implications on possible origin of these viroids in the Mediterranean region. J. Plant Pathol. 83:51-62.
10. Kyriakopoulou, P.E. and Hadidi, A. 1998. Natural infection of wild and cultivated pears with apple scar skin in Greece. Acta Hort. 472:617-525.
11. Manganaris, G.A., Economou, A.S., Boubourakas, I.N. and Katis, N.I. 2003. Elimination of PPV and PNRSV through thermotherapy and meristem-tip culture in nectarine. Plant Cell Reports 22:195-200
12. McDowell, D.G., Burns, N.A. and Parkes, H.C. 1998. Localized sequence regions high melting temperatures prevent the amplification of a DNA mimic in competitive PCR. Nucl. Acid Res. 26:3340-3347.
13. Ragozzino, E. and Faggioli, F. 2002. Detection of pome fruit viroids by RT-PCR using a single primer pair. J. Plant Pathol. 84(2):125-128.
14. Ragozzino, E., Faggioli, F. and Barba, M. 2004. Development of a one tube-one stepRT-PCR protocol for the detection of seven viroids in four genera: Apscaviroid, Hostuviroid, Pelamoviroid and Pospoviroid. J. Virol. Meth.
15. Rott, M.E. and Jelkmann, W. 2001. Characterization and detection of several filamentous viruses of cherry: Adaptation of an alternative cloning method (DOP-PCR), and modification of an RNA extraction protocol. E. J. Plant Pathol. 107:411-420.
16. Roux, K.H. 1995. Optimization and troubleshooting in PCR. p.55-66. In: C.W. Dieffenbach and G.S. Dveksler (eds.), PCR primer - A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
17. Rowhani, A., Maningas, M.A., Lile, L.S., Daubert, S.D. and Golino, D.A. 1995. Development of a detection system for viruses of woody plants based on analysis of immobilized virions. Phytopathology 85:347-351.
18. Shamloul, A.M., Faggioli, F., Keith, J.M. and Hadidi, A. 2002. A novel multiplex RT-PCR probe capture hybridization (RT-PCR-ELISA) for simultaneous detection of six viroids in four genera: Apscaviroid, Hostuviroid, Pelamoviroid and Pospiviroid. J. Virol. Meth. 105:115-121.
19. Zuker, M. 2003. Mfold web server for nucleic acid folding and hybridization prediction. Nucl. Acid Res. 31:3406-3415.