Characterization and growth of polymorphic Rickettsia felis in a tick cell line

Applied and Environmental Microbiology

Volumn 74, Issue 10, 2008, Pages 3151-3158

  Sunyakumthorn, Piyanate ^a   Bourchookarn, Apichai ^a,b   Pornwiroon, Walairat ^a   David, Connie ^a   Barker, Steven A ^a   Macaluso, Kevin R ^a,c  

^a LOUISIANA STATE UNIVERSITY   (United States)

^b PRINCE OF SONGKLA UNIVERSITY   (Thailand)

^c LOUISIANA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; CELL GROWTH; DNA; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MICROBIOLOGY;

BACTERIAL VIRULENCE; MULTIPLICITY OF INFECTION (MOI); RICKETTSIAL POLYMORPHISM; TRANSMISSION POTENTIAL;

BACTERIA;

BACTERIUM; GROWTH RATE; IDENTIFICATION METHOD; MICROSCOPY; MORPHOLOGY; PARASITE TRANSMISSION; POLYMERASE CHAIN REACTION; PROTEOMICS; SURVIVORSHIP; TICK; ULTRASTRUCTURE; VIRULENCE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL TRANSMISSION; BACTERIUM CULTURE; CELL GROWTH; CELL LINE; COMPARATIVE STUDY; DNA SEQUENCE; ELECTRON MICROSCOPY; GENETIC POLYMORPHISM; IMMUNOFLUORESCENCE; INFECTION; LIQUID CHROMATOGRAPHY; MEMBRANE STRUCTURE; MORPHOLOGY; NONHUMAN; POLYMERASE CHAIN REACTION; RICKETTSIA FELIS; TANDEM MASS SPECTROMETRY; TICK; WESTERN BLOTTING; ANIMAL; BACTERIAL COUNT; CHEMISTRY; CYTOLOGY; FLUORESCENCE MICROSCOPY; GENETIC VARIABILITY; GENETICS; GROWTH, DEVELOPMENT AND AGING; MASS SPECTROMETRY; MICROBIOLOGY;

ARTHROPODA; BACTERIA (MICROORGANISMS); IXODIDA; RICKETTSIA FELIS; RICKETTSIACEAE;

BACTERIAL DNA; BACTERIAL PROTEIN; PROTEOME;

ANIMALS; BACTERIAL PROTEINS; BLOTTING, WESTERN; CELL LINE; CHROMATOGRAPHY, LIQUID; COLONY COUNT, MICROBIAL; DNA, BACTERIAL; MASS SPECTROMETRY; MICROSCOPY, ELECTRON; MICROSCOPY, FLUORESCENCE; POLYMERASE CHAIN REACTION; PROTEOME; RICKETTSIA FELIS; SEQUENCE ANALYSIS, DNA; TICKS; VARIATION (GENETICS);

EID: 44249117632     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.00025-08     Document Type: Article

References (43)

1. Adams, J. R., E. T. Schmidtmann, and A. F. Azad. 1990. Infection of colonized cat fleas, Ctenocephalides felis (Bouche), with a rickettsia-like microorganism. Am. J. Trop. Med. Hyg. 43:400-409.
2. Azad, A. F., S. Radulovic, J. A. Higgins, B. H. Noden, and J. M. Troyer. 1997. Flea-borne rickettsioses: ecologic considerations. Emerg. Infect. Dis. 3:319-327.
3. Baldridge, G. D., N. Y. Burkhardt, R. F. Felsheim, T. J. Kurtti, and U. G. Munderloh. 2008. Plasmids of the pRM/pRF family occur in diverse Rickettsia species. Appl. Environ. Microbiol. 74:645-652.
4. Baldridge, G. D., N. Y. Burkhardt, R. F. Felsheim, T. J. Kurtti, and U. G. Munderloh. 2007. Transposon insertion reveals pRM, a plasmid of Rickettsia monacensis. Appl. Environ. Microbiol. 73:4984-4995.
5. Blouin, E. F., and K. M. Kocan. 1998. Morphology and development of Anaplasma marginale (Rickettsiales: Anaplasmataceae) in cultured Ixodes scapularis (Acari: Ixodidae) cells. J. Med. Entomol. 35:788-797.
6. Boostrom, A., M. S. Beier, J. A. Macaluso, K. R. Macaluso, D. Sprenger, J. Hayes, S. Radulovic, and A. F. Azad. 2002. Geographic association of Rickettsia felis-infected opossums with human murine typhus, Texas. Emerg. Infect. Dis. 8:549-554.
7. Bouyer, D. H., J. Stenos, P. Crocquet-Valdes, C. G. Moron, V. L. Popov, J. E. Zavala-Velazquez, L. D. Foil, D. R. Stothard, A. F. Azad, and D. H. Walker. 2001. Rickettsia felis: molecular characterization of a new member of the spotted fever group. Int. J. Syst. Evol. Microbiol. 51:339-347.
8. Burgdorfer, W., S. F. Hayes, and A. J. Marvos. 1981. Nonpathogenic rickettsiae in Dermacentor andersoni: a limiting factor for the distribution of Rickettsia rickettsii, p. 585-594. In W. Burgdorfer and R. L. Anacker (ed.), Rickettsiae and rickettsial diseases. Academic Press, New York, NY.
9. Case, J. B., B. Chomel, W. Nicholson, and J. E. Foley. 2006. Serological survey of vector-borne zoonotic pathogens in pet cats and cats from animal shelters and feral colonies. J. Feline Med. Surg. 8:111-117.
10. Coleman, S. A., E. R. Fischer, D. Howe, D. J. Mead, and R. A. Heinzen. 2004. Temporal analysis of Coxiella burnetii morphological differentiation. J. Bacteriol. 186:7344-7352.
11. Gulevskaia, S. A., V. L. Popov, and V. F. Ignatovich. 1975. Current data on the polymorphism of Rickettsia prowazekii and burneti in cultured cells. Zh. Mikrobiol. Epidemiol. Immunobiol. 7:68-72. (In Russian.)
12. Heinzen, R. A., T. Hackstadt, and J. E. Samuel. 1999. Developmental biology of Coxiella burnettii. Trends Microbiol. 7:149-154.
13. Horta, M. C., M. B. Labruna, E. L. Durigon, and T. T. Schumaker. 2006. Isolation of Rickettsia felis in the mosquito cell line C6/36. Appl. Environ. Microbiol. 72:1705-1707.
14. Ito, S., and Y. Rikihisa. 1981. Techniques for electron microscopy of rickettsiae, p. 213-240. In W. Burgdorfer and R. L. Anacker (ed.), Rickettsiae and rickettsial diseases. Academic Press, Inc., New York, NY.
15. Kekcheeva, N. G., I. N. Kokorin, V. L. Popov, E. A. Chereshkova, N. S. Smirnova, O. A. Vovk, and E. M. Shirokova. 1992. Persistence of Rickettsia prowazekii in cotton rat macrophage cultures. Acta Virol. 36:103-110.
16. Kocan, K. M. 1986. Development of Anaplasma marginale Theiler in ixodid ticks: Coordinated development of a rickettsial organism and its tick host, p. 472-505. In J. R. Sauer and J. A. Hair (ed.), Morphology, physiology, and behavioral biology of ticks. John Wiley & Sons, New York, NY.
17. Kocan, K. M. 1992. Recent advances in the biology of Anaplasma spp. in Dermacentor andersoni ticks. Ann. N. Y. Acad. Sci. 653:26-32.
18. Kocan, K. M., S. A. Ewing, D. Holbert, and J. A. Hair. 1982. Morphologic characteristics of colonies of Anaplasma marginale Theiler in midgut epithelial cells of Dermacentor andersoni Stiles. Am. J. Vet. Res. 43:586-593.
19. Kocan, K. M., W. L. Goff, D. Stiller, P. L. Claypool, W. Edwards, S. A. Ewing, J. A. Hair, and S. J. Barron. 1992. Persistence of Anaplasma marginale (Rickettsiales: Anaplasmataceae) in male Dermacentor andersoni (Acari: Ixodidae) transferred successively from infected to susceptible calves. J. Med. Entomol. 29:657-668.
20. Kocan, K. M., D. Holbert, W. Edwards, S. A. Ewing, S. J. Barron, and J. A. Hair. 1986. Longevity of colonies of Anaplasma marginale in midgut epithelial cells of Dermacentor andersoni. Am. J. Vet. Res. 47:1657-1661.
21. Kurtti, T. J., J. A. Simser, G. D. Baldridge, A. T. Palmer, and U. G. Munderloh. 2005. Factors influencing in vitro infectivity and growth of Rickettsia peacockii (Rickettsiales: Rickettsiaceae), an endosymbiont of the Rocky Mountain wood tick, Dermacentor andersoni (Acari, Ixodidae). J. Invertebr. Pathol. 90:177-186.
22. Labruna, M. B., R. C. Pacheco, L. J. Richtzenhain, and M. P. Szabo. 2007. Isolation of Rickettsia rhipicephali and Rickettsia bellii from Haemaphysalis juxtakochi ticks in the state of Sao Paulo, Brazil. Appl. Environ. Microbiol. 73:869-873.
23. Labruna, M. B., T. Whitworth, M. C. Horta, D. H. Bouyer, J. W. McBride, A. Pinter, V. Popov, S. M. Gennari, and D. H. Walker. 2004. Rickettsia species infecting Amblyomma cooperi ticks from an area in the state of Sao Paulo, Brazil, where Brazilian spotted fever is endemic. J. Clin. Microbiol. 42:90-98.
24. Macaluso, K. R., W. Pornwiroon, V. Popov, and L. D. Foil. 2008. Identification of Rickettsia felis in the salivary glands of cat fleas. Vector Borne Zoonotic Dis., in press.
25. Munderloh, U. G., S. D. Jauron, V. Fingerle, L. Leitritz, S. F. Hayes, J. M. Hautman, C. M. Nelson, B. W. Huberty, T. J. Kurtti, G. G. Ahlstrand, B. Greig, M. A. Mellencamp, and J. L. Goodman. 1999. Invasion and intracellular development of the human granulocytic ehrlichiosis agent in tick cell culture. J. Clin. Microbiol. 37:2518-2524.
26. Munderloh, U. G., and T. J. Kurtti. 1989. Formulation of medium for tick cell culture. Exp. Appl. Acarol. 7:219-229.
27. Munderloh, U. G., and T. J. Kurtti. 1995. Cellular and molecular interrelationships between ticks and prokaryotic tick-borne pathogens. Annu. Rev. Entomol. 40:221-243.
28. Ogata, H., P. Renesto, S. Audic, C. Robert, G. Blanc, P. E. Fournier, H. Parinello, J. M. Claverie, and D. Raoult. 2005. The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite. PLoS. Biol. 3:e248.
29. Pang, H., and H. H. Winkler. 1994. Analysis of the peptidoglycan of Rickettsia prowazekii. J. Bacteriol. 176:923-926.
30. Parola, P., B. Davoust, and D. Raoult. 2005. Tick- and flea-borne rickettsial emerging zoonoses. Vet. Res. 36:469-492.
31. Philip, R. N., and E. A. Casper. 1981. Serotypes of spotted fever group rickettsiae isolated from Dermacentor andersoni (Stiles) ticks in western Montana. Am. J. Trop. Med. Hyg. 30:230-238.
32. Philip, R. N., E. A. Casper, R. L. Anacker, J. Cory, S. F. Hayes, W. Burgdorfer, and C. E. Yunker. 1983. Rickettsia bellii sp. nov.: a tick-borne rickettsia, widely distributed in the United States, that is distinct from the spotted fever and typhus biogroups. Int. J. Syst. Bacteriol. 33:94-106.
33. Popov, V. L., V. C. Han, S. M. Chen, J. S. Dumler, H. M. Feng, T. G. Andreadis, R. B. Tesh, and D. H. Walker. 1998. Ultrastructural differentiation of the genogroups in the genus Ehrlichia. J. Med. Microbiol. 47:235-251.
34. Pornwiroon, W., M. T. Kearney, C. Husseneder, L. D. Foil, and K. R. Macaluso. 2007. Comparative microbiota of Rickettsia felis-uninfected and -infected colonized cat fleas, Ctenocephalides felis. ISME J. 1:394-402.
35. Pornwiroon, W., S. S. Pourciau, L. D. Foil, and K. R. Macaluso. 2006. Rickettsia felis from cat fleas: isolation and culture in a tick-derived cell line. Appl. Environ. Microbiol. 72:5589-5595.
36. Raoult, D., S. B. La, M. Enea, P. E. Fournier, V. Roux, F. Fenollar, M. A. Galvao, and X. L. de. 2001. A flea-associated Rickettsia pathogenic for humans. Emerg. Infect. Dis. 7:73-81.
37. Rikihisa, Y., and S. Ito. 1980. Localization of electron-dense tracers during entry of Rickettsia tsutsugamushi into polymorphonuclear leukocytes. Infect. Immun. 30:231-243.
38. Sakamoto, J. M., and A. F. Azad. 2007. Propagation of arthropod-borne Rickettsia spp. in two mosquito cell lines. Appl. Environ. Microbiol. 73:6637-6643.
39. Schriefer, M. E., J. B. Sacci, Jr., J. P. Taylor, J. A. Higgins, and A. F. Azad. 1994. Murine typhus: updated roles of multiple urban components and a second typhuslike rickettsia. J. Med. Entomol. 31:681-685.
40. Simser, J. A., A. T. Palmer, U. G. Munderloh, and T. J. Kurtti. 2001. Isolation of a spotted fever group Rickettsia, Rickettsia peacockii, in a Rocky Mountain wood tick, Dermacentor andersoni, cell line. Appl. Environ. Microbiol. 67:546-552.
41. Wedincamp, J., Jr., and L. D. Foil. 2002. Vertical transmission of Rickettsia felis in the cat flea (Ctenocephalides felis Bouche). J. Vector Ecol. 27:96-101.
42. Williams, S. G., J. B. Sacci, Jr., M. E. Schriefer, E. M. Andersen, K. K. Fujioka, F. J. Sorvillo, A. R. Barr, and A. F. Azad. 1992. Typhus and typhuslike rickettsiae associated with opossums and their fleas in Los Angeles County, California. J. Clin. Microbiol. 30:1758-1762.
43. Wisseman, C. L., Jr., and A. D. Waddell. 1975. In vitro studies on rickettsia-host cell interactions: intracellular growth cycle of virulent and attenuated Rickettsia prowazeki in chicken embryo cells in slide chamber cultures. Infect. Immun. 11:1391-1404.