Shaken helical track bioreactors: Providing oxygen to high-density cultures of mammalian cells at volumes up to 1000 L by surface aeration with air

New Biotechnology

Volumn 25, Issue 1, 2008, Pages 68-75

  Zhang, Xiaowei ^a   Stettler, Matthieu ^a   Reif, Oscar ^b   Kocourek, Andreas ^b   DeJesus, Maria ^c   Hacker, David L ^a   Wurm, Florian M ^a,c  

^a EPFL   (Switzerland)

^b SARTORIUS STEDIM BIOTECH GMBH   (Germany)

^c EXCELLGENE SA   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE; CELL GROWTH; MAMMALS; MASS TRANSFER; PHASE INTERFACES;

CELL GROWTH PROFILES; MAMMALIAN CELLS; SURFACE AERATION;

BIOREACTORS;

AERATION; ANIMAL CELL; BIOREACTOR; CELL CULTURE; CELL DENSITY; CELL GROWTH; CHO CELL; CONTROLLED STUDY; FEMALE; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; REVIEW; SHAKEN HELICAL TRACK BIOREACTOR; VOLUMETRY;

AIR; ANIMALS; BIOREACTORS; CELL COUNT; CELL PROLIFERATION; CELLS, CULTURED; CHO CELLS; CRICETINAE; CRICETULUS; OXYGEN; RHEOLOGY;

MAMMALIA;

EID: 44049095731     PISSN: None     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.nbt.2008.03.001     Document Type: Review

References (25)

1. Wurm F.M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 22 (2004) 1393-1397
2. Büchs J. Introduction to advantages and problems of shaken cultures. Biochem. Eng. J. 7 (2001) 91-98
3. Liu C.M., and Hong L.N. Development of a shaking bioreactor system for animal cell cultures. Biochem. Eng. J. 7 (2001) 121-125
4. DeJesus M.J., et al. Tubespin satellites: a fast track approach for process development with animal cells using shaking technology. Biochem. Eng. J. 17 (2004) 217-223
5. Muller N., et al. Orbital shaker technology for the cultivation of mammalian cells in suspension. Biotechnol. Bioeng. 89 (2005) 400-406
6. Micheletti M., et al. Fluid mixing in shaken bioreactors: implications for scale-up predictions from microliter-scale microbial and mammalian cell cultures. Chem. Eng. Sci. 61 (2006) 2939-2949
7. Muller N., et al. Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems. Biotechnol. Lett. 29 (2007) 703-711
8. Stettler M., et al. Novel orbital shake bioreactors for transient production of CHO derived IgGs. Biotechnol. Prog. 23 (2007) 1340-1346
9. Marks D.M. Equipment design considerations for large scale cell culture. Cytotechnology 42 (2003) 21-33
10. Garcia-Ocuoa F., and Gomez E. Prediction of gas-liquid mass transfer coefficient in sparged stirred tank bioreactors. Biotechnol. Bioeng. 92 (2005) 761-772
11. Kato Y., et al. Measurement of mass transfer rate from free surface in shaking vessel type bioreactor. J. Chem. Eng. Jap. 30 (1997) 362-365
12. Maier U., and Büchs J. Characterization of the gas-liquid mass transfer in shaking bioreacors. Biochem. Eng. J. 7 (2001) 99-106
13. Anderlei T., and Büchs J. Device for sterile online measurement of the oxygen transfer rate in shaking flasks. Biochem. Eng. J. 7 (2001) 157-162
14. Gupta A., and Rao G. A study of oxygen transfer in shake flasks using a non-invasive oxygen sensor. Biotechnol. Bioeng. 84 (2003) 351-358
15. Zhang H., et al. Computational-fluid-dynamics (CFD) analysis of mixing and gas-liquid mass transfer in shake flasks. Biotechnol. Appl. Biochem. 41 (2005) 1-8
16. Nienow A.W. Reactor engineering in large scale animal cell culture. Cytotechnology 50 (2006) 9-33
17. 2 incubator. Cytotechnology 14 (1994) 1-9
18. Suijdam J.C.V., et al. Model for oxygen transfer in a shake flask. Biotechnol. Bioeng. 20 (1978) 1695-1709
19. Kato Y., et al. Effects of liquid film formed on flask surface on oxygen transfer rate in shaking flask and development of baffled shaking vessel by optical method based on sulfite oxidation. J. Chem. Eng. Jap. 38 (2005) 873-877
20. Deshpande R.R., and Heinzle E. On-line oxygen uptake rate and culture viability measurement of animal cell culture using microplates with integrated oxygen sensors. Biotechnol. Lett. 26 (2004) 763-767
21. Varley J., and Birch J. Reactor design for large scale suspension animal cell culture. Cytotechnology 29 (1999) 177-205
22. Moreira J.L., et al. Influence of power input and aeration method on mass transfer in a laboratory animal cell culture vessel. J. Chem. Technol. Biotechnol. 62 (1995) 118-131
23. Büchs J., et al. Calculation liquid distribution in shake flasks on rotary shakers at waterlike viscosities. Biochem. Eng. J. 34 (2007) 200-208
24. Senger R.S., and Karim M.N. Effect of shear stress on intrinsic CHO culture state and glycosylation of recombinant tissue-type plasminogen activator protein. Biotechnol. Prog. 19 (2003) 1199-1209
25. Peter P.C., et al. Hydromechanical stress in shake flasks: correlation for the maximum local energy dissipation rate. Biotechnol. Bioeng. 93 (2006) 1164-1176