Expression of Aminopeptidase N1 (APN1), the Main Receptor Protein for Bacillus thuringiensis Cry1A Toxin from Helicoverpa armigera Larval Midgut in Trichoplusia ni cells

Agricultural Sciences in China

Volumn 7, Issue 3, 2008, Pages 329-335

  CHANG, Hong lei ^a   LIANG, Ge mei ^a   WANG, Gui rong ^a   YU, Hong kun ^a   GUO, Yu yuan ^a   WU, Kong ming ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

APN1; baculovirus expression vector; expression; Trichoplusia ni

Indexed keywords

BACILLUS THURINGIENSIS; ESCHERICHIA COLI; EUKARYOTA; GOSSYPIUM HIRSUTUM; HELICOVERPA ARMIGERA; HEXAPODA; TRICHOPLUSIA NI;

EID: 44049090260     PISSN: 16712927     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1671-2927(08)60073-5     Document Type: Article

References (24)

1. Agrawal N., Malhotra P., and Bhatnagar R. Interaction of gene-cloned and insect cell-expressed aminopeptidase N of Spodoptera litura with insecticidal crystal protein Cry1C. Applied and Environmental Microbiology 68 (2002) 4583-4592
2. Banks D., Hua G., and Adang M. Cloning of a Heliothis virescens 110 kDa aminopeptidase N and expression in Drosophila S2 cells. Insect Biochemistry and Molecular Biology 33 (2003) 499-508
3. Bravo A., Gomez I., Conde J., Garay C., Sanchez J., Miranda R., Zhuang M., Gill S., and Soberon M. Oligomerization triggers binding of a Bacillus thuringiensis Cry1Ab poreforming toxin to aminopeptidase N receptor leading to insertion into membrane microdomains. Biochimical et Biophysica Acta 1667 (2004) 38-46
4. Chang H., Liang G., Yu H., Wang G., Wu K., and Guo Y. Relationships between aminopeptidase N - the receptor for Bt toxin in Helicoverpa armigera and Bt resistance. Plant Protection 33 (2007) 1-5 (in Chinese)
5. Emmerling M., Chandler D., and Sandeman M. Molecular cloning of three cDNAs encoding aminopeptidases from the midgut of Helicoverpa punctigera, the Australian native budworm. Insect Biochemistry and Molecular Biology 31 (2001) 899-907
6. Gahan L., Gould F., and Heckel D. Identification of a gene associated with Bt resistance in Heliothis virescens. Science 293 (2001) 857-860
7. Gill M., and Ellar D. Transgenic Drosophila reveals a functional in vivo receptor for Bacillus thuringiensis toxin Cry 1Ac. Insect Molecular Biology 11 (2002) 619-625
8. Gilliland A., Chambers C., Bone E., and Ellar D. Role of Bacillus thuringiensis Cry 1δ-endotoxin binding in determining potency during lepidopteran larval development. Applied and Environmental Microbiology 68 (2002) 1509-1515
9. Griffitts J., Haslam S., Yang T., Garczynski S., Mulloy B., Morris H., Cremer P., Dell A., Adang M., and Aroian R. Glycolipids as receptors for Bacillus thuringiensis crystal toxin. Science 307 (2005) 922-925
10. Jurat-Fuentes J., and Adang M. Characterization of a Cry1Ac-receptor alkaline phosphatase in susceptible and resistant Heliothis virescens larvae. European Journal Biochemistry 271 (2004) 3127-3135
11. Knight P., Carroll J., and Ellar D. Analysis of glycan structures on the 120 kDa aminopeptidase N of Manduca sexta and their interactions with Bacillus thuringiensis Cry1Ac toxin. Insect Biochemistry and Molecular Biology 34 (2003) 101-112
12. Kost T., Condreay J., and Jarvis D. Baculovirus as versatile vectors for protein express in insect and mammalian cells. Nature Biotechnology 23 (2005) 567-575
13. Krishnamoorthy M., Jurat-Fuentes J., McNall R., Andacht T., and Adang M. Identification of novel Cry1Ac binding proteins in midgut membranes from Heliothis virescens using proteomic analyses. Insect Biochemistry and Molecular Biology 37 (2007) 189-201
14. Liang G., Tan W., and Guo Y. Studies on the resistance screening and cross-resistance of cotton boilworm to Bacillus thuringiensis (Berliner). Scientia Agricultura Sinica 33 (2000) 46-53 (in Chinese)
15. Liang G., Wang G., Xu G., Wu K., and Guo Y. Purrification of aminopeptidase N protein and differences in cDNAs encoding APN1 between susceptible and resistant Helicoverpa armigera strains to Bacillus thuringiensis toxins. Agricultural Sciences in China 3 (2004) 456-467
16. Luo S., Wang G., Liang G., Wu K., Bai L., Ren X., and Guo Y. Binding of three Cry1A toxins in resistant and susceptible strains of cotton bollworm (Helicoverpa armigera). Pesticide Biochemistry and Physiology 85 (2006) 104-109
17. McNall R., and Adang M. Identification of novel Bacillus thuringiensis Cry1Ac binding proteins in Manduca Sexta midgut through proteomic analysis. Insect Biochemistry and Molecular Biology 33 (2003) 999-1010
18. Rajagopal R., Agrawal N., Selvapandiyan A., Sivakumar S., Ahmad S., and Bhatnagar R. Recombinantly expressed isoenzymic aminopeptidases from Helicoverpa armigera (American cotton bollworm) midgut display differential interaction with closely related Bacillus thuringiensis insecticidal proteins. Biochemical Journal 370 (2003) 971-978
19. Rajagopal R., Sivakumar S., Agrawal N., Malhotra P., and Bhatnagar R. Silencing of midgut Aminopeptidase N of Spodoptera litura by double-stranded RNA establishes its role as Bacillus thuringiensis toxin receptor. The Journal of Biological Chemistry 277 (2002) 46849-46851
20. Simpson R., and Newcomb R. Binding of Bacillus thuringiensis δ-endotoxin Cry1Ac and Cry1Ba to 120 kDa aminopeptidase N of Epiphyas postvittana purified from both brush border membrane vesicles and baculovirus infected Sf9 cells. Insect Biochemistry and Molecular Biology 30 (2000) 1096-1178
21. Wang G., Liang G., Wu K., and Guo Y. Cloning and sequencing of a gene encoding Aminopeptidase N in the midgut of Helicoverpa armigera (Hübner). Agricultural Sciences in China 2 (2003) 760-767
22. Whalon M., and Wingerd B. Bt: Mode of action and use. Archives of Insect Biochemistry Physiology 54 (2003) 200-211
23. Yaoi K., Nakanishi K., Kadotani T., Imamura M., Koizumi N., Iwahana H., and Sato R. cDNA cloning and expression of Bacillus thuringiensis Cry1Aa toxin binding 120 kDa aminopeptidase N from Bombyx mori. Biochemica et Biophysica Acta 1444 (1998) 131-137
24. Zhu Y., Kramer K., Oppert B., and Dowdy A. cDNAs of aminopeptidase-like protein genes from Plodia interpunctlla strains with different susceptibilities to Bacillus thuringiensis toxin. Insect Biochemistry and Molecular Biology 30 (2000) 215-224