Dual-targeted tRNA-dependent amidotransferase ensures both mitochondrial and chloroplastic Gln-tRNAGln synthesis in plants

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 17, 2008, Pages 6481-6485

  Pujol, Claire ^a   Bailly, Marc ^b   Kern, Daniel ^b   Maréchal Drouard, Laurence ^a   Becker, Hubert ^b   Duchêne, Anne Marie ^a  

^a INST DE BIOL MOLEC DES PLANTES   (France)

^b CNRS   (France)

Author keywords

Amidation; Aminoacylation; GatCAB; Glutamyl tRNA synthetase; Protein trafficking

Indexed keywords

GLUTAMINE TRANSFER RNA; PROTEIN GAT; PROTEIN SUBUNIT; ZINC FINGER PROTEIN; AMINOACYL TRANSFER RNA; CELL EXTRACT; GLUTAMATE TRANSFER RNA LIGASE; GLUTAMINE; TRANSFERASE; TRNA, GLUTAMINE; TRNA, GLUTAMINE-;

AMIDATION; ARABIDOPSIS; ARTICLE; CELL ORGANELLE; CHLOROPLAST; CONTROLLED STUDY; CYTOSOL; GENE EXPRESSION; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; BIOSYNTHESIS; ENZYMOLOGY; METABOLISM; POTATO; PROTEIN TRANSPORT;

EUKARYOTA; FUNGI; PROTISTA;

ARABIDOPSIS; CELL EXTRACTS; CHLOROPLASTS; CYTOSOL; GLUTAMATE-TRNA LIGASE; GLUTAMINE; MITOCHONDRIA; NITROGENOUS GROUP TRANSFERASES; PROTEIN SUBUNITS; PROTEIN TRANSPORT; RNA, TRANSFER, AMINO ACYL; SOLANUM TUBEROSUM;

EID: 44049088184     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0712299105     Document Type: Article

References (22)

1. Ibba M, Becker HD, Stathopoulos C, Tumbula DL, Söll D (2000) The adaptor hypothesis revisited. Trends Biochem Sci 25:311-316.
2. Nabholz C, Hauser R, Schneider A (1997) Leishmania tarentolae contains distinct cytosolic and mitochondrial glutaminyl-tRNA synthetase activities. Proc Natl Acad Sci USA 94:7903-7908.
3. Rinehart J, Horn EK, Wei D, Söll D, Schneider A (2004) Non-canonical eukaryotic glutaminyl- and glutamyl-tRNA synthetases form mitochondrial aminoacyl-tRNA in Trypanosoma brucei. J Biol Chem 279:1161-1166.
4. Rusconi C, Cech T (1996) The anticodon is the signal sequence for mitochondrial import of glutamine tRNA in Tetrahymena. Genes Dev 10:2870-2880.
5. Rinehart J, Krett B, Rubio MA, Alfonzo JD, Söll D (2005) Saccharomyces cerevisiae imports the cytosolic pathway for Gln-tRNA synthesis into the mitochondrion. Genes Dev 19:583-592.
6. Schon A, Kannangara CG, Gough S, Söll D (1988) Protein biosynthesis in organelles requires misaminoacylation of tRNA. Nature 331:187-190.
7. Duchêne AM, Maréchal-Drouard L (2001) The chloroplast-derived trnW and trnM-e genes are not expressed in Arabidopsis mitochondria. Biochem Biophys Res Commun 285:1213-1216.
8. Duchêne AM, et al. (2005) Dual targeting is the rule for organellar aminoacyl-tRNA synthetases in Arabidopsis thaliana. Proc Natl Acad Sci USA 102:16484-16489.
9. Bailly M, et al. (2006) A single tRNA base pair mediates bacterial tRNA-dependent biosynthesis of asparagine. Nucleic Acids Res 34:6083-6094.
10. Sheppard K, Akochy PM, Salazar JC, Söll D (2007) The Helicobacter pylori amidotransferase GatCAB is equally efficient in glutamine-dependent transamidation of Asp-tRNAAsn and Glu-tRNAGln. J Biol Chem 282:11866-11873.
11. Pujol C, Maréchal-Drouard L, Duchêne AM (2007) How can organellar protein N-terminal sequences be dual targeting signals? In silico analysis and mutagenesis approach. J Mol Biol 369:356-367.
12. Peeters NM, et al (2000) Duplication and quadruplication of Arabidopsis thaliana cysteinyl- and asparaginyl-tRNA synthetase genes of organellar origin. J Mol Evol 50:413-423.
13. Brubacher-Kauffmann S, Maréchal-Drouard L, Cosset A, Dietrich A, Duchêne AM (1999) Differential import of nuclear-encoded tRNAGly isoacceptors into Solanum tuberosum mitochondria. Nucleic Acids Res 27:2037-2042.
14. Curnow AW, et al. (1997) Glu-tRNAGln amidotransferase: a novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation. Proc Natl Acad Sci USA 94:11819-11826.
15. Nakamura A, Yao M, Chimnaronk S, Sakai N, Tanaka I (2006) Ammonia channel couples glutaminase with transamidase reactions in GatCAB. Science 312:1954-1958.
16. Mulero JJ, Rosenthal JK, Fox TD (1994) PET112, a Saccharomyces cerevisiae nuclear gene required to maintain rho+ mitochondrial DNA. Curr Genet 25:299-304.
17. Hughes TR, et al. (2000) Functional discovery via a compendium of expression profiles. Cell 102:109-126.
18. Small I, et al. (1999) The strange evolutionary history of plant mitochondrial tRNAs and their aminoacyl-tRNA synthetases. J Hered 90:333-337.
19. Seki M, et al. (2002) Functional annotation of a full-length Arabidopsis cDNA collection. Science 296:141-145.
20. Asn. Methods 44:146-163.
21. Maréchal-Drouard L, Small I, Weil JH, Dietrich A (1995) Transfer RNA import into plant mitochondria. Methods Enzymol 260:310-327.
22. Bonnefond L, et al. (2005) Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS. Biochemistry 44:4805-4816.