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Volumn 98, Issue 3, 2008, Pages 271-279

Application of denaturing gradient gel electrophoresis for analysing the gut microflora of Lumbricus rubellus Hoffmeister under different feeding conditions

Author keywords

Band identification; Gut microbiota; Lumbricus rubellus Hoffmeister; Microbial community composition; Polymerase chain reaction denaturing gradient gel electrophoresis (PCR DGGE) analysis

Indexed keywords

BACTERIAL DNA; FUNGAL DNA; RIBOSOME DNA;

EID: 43749087060     PISSN: 00074853     EISSN: 14752670     Source Type: Journal    
DOI: 10.1017/S0007485308006056     Document Type: Conference Paper
Times cited : (24)

References (47)
  • 1
    • 0032401857 scopus 로고    scopus 로고
    • Linking above-ground and below-ground interactions: How plant responses to foliar herbivory influences soil organisms
    • Bardgett, R.D., Wardle, D.A. & Yea, G.W. (1998) Linking above-ground and below-ground interactions: How plant responses to foliar herbivory influences soil organisms. Soil Biology Biochemistry 30, 1867-1878.
    • (1998) Soil Biology Biochemistry , vol.30 , pp. 1867-1878
    • Bardgett, R.D.1    Wardle, D.A.2    Yea, G.W.3
  • 2
    • 84962929264 scopus 로고    scopus 로고
    • Functional interactions between earthworms, microorganisms, organic matter, and plants
    • Edwards, C.A, Ed, Boca Raton, FL, USA, CRC Press LLC
    • Brown, G.G. & Doube, B.M. (2004) Functional interactions between earthworms, microorganisms, organic matter, and plants. pp. 213-239 in Edwards, C.A. (Ed.) Earthworm Ecology. Boca Raton, FL, USA, CRC Press LLC.
    • (2004) Earthworm Ecology , pp. 213-239
    • Brown, G.G.1    Doube, B.M.2
  • 3
    • 0034490377 scopus 로고    scopus 로고
    • Regulation of soil organic matter dynamics and microbial activity in the drilosphere and the role of interactions with other edaphic functional domains
    • Brown, G.G., Barois, I. & Lavelle, P. (2000) Regulation of soil organic matter dynamics and microbial activity in the drilosphere and the role of interactions with other edaphic functional domains. European Journal of Soil Biology 36, 177-198.
    • (2000) European Journal of Soil Biology , vol.36 , pp. 177-198
    • Brown, G.G.1    Barois, I.2    Lavelle, P.3
  • 4
    • 0036223838 scopus 로고    scopus 로고
    • Fate of protozoa transiting the digestive tract of the earthworm Lumbricus terrestris L
    • Cai, H., Zarda, B., Mattison, G.R., Schönholzer, F. & Hahn, D. (2002) Fate of protozoa transiting the digestive tract of the earthworm Lumbricus terrestris L. Pedobiologia 46, 161-175.
    • (2002) Pedobiologia , vol.46 , pp. 161-175
    • Cai, H.1    Zarda, B.2    Mattison, G.R.3    Schönholzer, F.4    Hahn, D.5
  • 5
    • 33644859364 scopus 로고    scopus 로고
    • Plants actively control nitrogen cycling: Uncorking the microbial bottleneck
    • Chapman, S.K., Langley, J.A., Hart, S.C. & Koch, G.W. (2006) Plants actively control nitrogen cycling: uncorking the microbial bottleneck. New Phytologist 169, 27-34.
    • (2006) New Phytologist , vol.169 , pp. 27-34
    • Chapman, S.K.1    Langley, J.A.2    Hart, S.C.3    Koch, G.W.4
  • 6
    • 33845207685 scopus 로고    scopus 로고
    • The feeding ecology of earthworms - A review
    • Curry, J.P. & Schmidt, O. (2007) The feeding ecology of earthworms - A review. Pedobiologia 50, 463-477.
    • (2007) Pedobiologia , vol.50 , pp. 463-477
    • Curry, J.P.1    Schmidt, O.2
  • 8
    • 2142825652 scopus 로고    scopus 로고
    • Molecular profiling of 16S rRNA genes reveals diet-related differences of microbial communities in soil, gut, and casts of Lumbricus terrestris L. (Oligochaeta: Lumbricidae)
    • Egert, M., Marhan, S., Wagner, B., Scheu, S. & Friedrich, M.W. (2004) Molecular profiling of 16S rRNA genes reveals diet-related differences of microbial communities in soil, gut, and casts of Lumbricus terrestris L. (Oligochaeta: Lumbricidae). FEMS Microbiology Ecology 48, 187-197.
    • (2004) FEMS Microbiology Ecology , vol.48 , pp. 187-197
    • Egert, M.1    Marhan, S.2    Wagner, B.3    Scheu, S.4    Friedrich, M.W.5
  • 9
    • 0036195306 scopus 로고    scopus 로고
    • Molecular and culture-based analyses of prokaryotic communities from an agricultural soil and the burrows and casts of the earthworm Lumbricus rubellus
    • Furlong, M.A., Singleton, D.R., Coleman, D.C. & Whitman, W.B. (2002) Molecular and culture-based analyses of prokaryotic communities from an agricultural soil and the burrows and casts of the earthworm Lumbricus rubellus. Applied and Environmental Microbiology 68, 1265-1279.
    • (2002) Applied and Environmental Microbiology , vol.68 , pp. 1265-1279
    • Furlong, M.A.1    Singleton, D.R.2    Coleman, D.C.3    Whitman, W.B.4
  • 10
  • 11
    • 18044402193 scopus 로고    scopus 로고
    • Soil micropore structure and carbon mineralization in burrows and casts of an anecic earthworm (Lumbricus terrestris)
    • Görres, J.H., Savin, M.C. & Amador, J.A. (2001) Soil micropore structure and carbon mineralization in burrows and casts of an anecic earthworm (Lumbricus terrestris). Soil Biology and Biochemistry 33, 1881-1887.
    • (2001) Soil Biology and Biochemistry , vol.33 , pp. 1881-1887
    • Görres, J.H.1    Savin, M.C.2    Amador, J.A.3
  • 13
    • 0030739128 scopus 로고    scopus 로고
    • Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients
    • Heuer, H., Krsek, M., Baker, P., Smalla, K. & Wellington, E.M.H. (1997) Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients. Applied and Environmental Microbiology 63, 3233-3241.
    • (1997) Applied and Environmental Microbiology , vol.63 , pp. 3233-3241
    • Heuer, H.1    Krsek, M.2    Baker, P.3    Smalla, K.4    Wellington, E.M.H.5
  • 14
    • 0027329702 scopus 로고
    • Scanning electron-microscopy of the gut microflora of two earthworms - Lumbricus terrestris and Octolasion cyaneum
    • Jolly, J.M., Lappinscott, H.M., Anderson, J.M. & Clegg, C.D. (1993) Scanning electron-microscopy of the gut microflora of two earthworms - Lumbricus terrestris and Octolasion cyaneum. Microbial Ecology 26, 235-245.
    • (1993) Microbial Ecology , vol.26 , pp. 235-245
    • Jolly, J.M.1    Lappinscott, H.M.2    Anderson, J.M.3    Clegg, C.D.4
  • 15
    • 12944263588 scopus 로고    scopus 로고
    • Detecting predation and scavenging by DNA gut-content analysis: A case study using a soil insect predator-prey system
    • Juen, A. & Traugott, M. (2005) Detecting predation and scavenging by DNA gut-content analysis: a case study using a soil insect predator-prey system. Oecologia 142, 344-352.
    • (2005) Oecologia , vol.142 , pp. 344-352
    • Juen, A.1    Traugott, M.2
  • 16
    • 0028948028 scopus 로고
    • Comparative assessment of the aerobic and anaerobic microfloras of earthworm guts and forest soils
    • Karsten, G.R. & Drake, H.L. (1995) Comparative assessment of the aerobic and anaerobic microfloras of earthworm guts and forest soils. Applied and Environmental Microbiology 61, 1039-1044.
    • (1995) Applied and Environmental Microbiology , vol.61 , pp. 1039-1044
    • Karsten, G.R.1    Drake, H.L.2
  • 18
    • 0036136956 scopus 로고    scopus 로고
    • Phylogeny of culturable estuarine bacteria catabolizing riverine organic matter in the northern Baltic sea
    • Kisand, V., Cuadros, R. & Wikner, J. (2002) Phylogeny of culturable estuarine bacteria catabolizing riverine organic matter in the northern Baltic sea. Applied and Environmental Microbiology 68, 379-388.
    • (2002) Applied and Environmental Microbiology , vol.68 , pp. 379-388
    • Kisand, V.1    Cuadros, R.2    Wikner, J.3
  • 19
    • 0034775280 scopus 로고    scopus 로고
    • Ammonia-oxidizing bacteria: A model for molecular Microbial Ecology
    • Kowalchuk, G.A. & Stephen, J.R. (2001) Ammonia-oxidizing bacteria: A model for molecular Microbial Ecology. Annual Review of Microbiology 55, 485-529.
    • (2001) Annual Review of Microbiology , vol.55 , pp. 485-529
    • Kowalchuk, G.A.1    Stephen, J.R.2
  • 20
    • 0030901922 scopus 로고    scopus 로고
    • Analysis of ammonia-oxidizing bacteria of the β subdivision of the class Proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCR-amplified 16S ribosomal DNA fragments
    • Kowalchuk, G.A., Stephen, J.R., de Boer, W., Prosser, J.I., Embley, T.M. & Woldendorp, J.W. (1997) Analysis of ammonia-oxidizing bacteria of the β subdivision of the class Proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCR-amplified 16S ribosomal DNA fragments. Applied and Environmental Microbiology 63, 1489-1497.
    • (1997) Applied and Environmental Microbiology , vol.63 , pp. 1489-1497
    • Kowalchuk, G.A.1    Stephen, J.R.2    de Boer, W.3    Prosser, J.I.4    Embley, T.M.5    Woldendorp, J.W.6
  • 21
    • 0027333211 scopus 로고
    • Actinomycete communities in earthworm guts and surrounding soil
    • Krištůfek, V., Ravasz, K. & Pižl, V. (1993) Actinomycete communities in earthworm guts and surrounding soil. Pedobiologia 37, 379-384.
    • (1993) Pedobiologia , vol.37 , pp. 379-384
    • Krištůfek, V.1    Ravasz, K.2    Pižl, V.3
  • 23
    • 0030661563 scopus 로고    scopus 로고
    • Characterisation of microbial diversity by determining terminal restriction length polymorphisms of genes encoding 16S rDNA
    • Liu, W.-T., Marsh, T.L., Cheng, H. & Forney, L.J. (1997) Characterisation of microbial diversity by determining terminal restriction length polymorphisms of genes encoding 16S rDNA. Applied and Environmental Microbiology 63, 4516-4522.
    • (1997) Applied and Environmental Microbiology , vol.63 , pp. 4516-4522
    • Liu, W.-T.1    Marsh, T.L.2    Cheng, H.3    Forney, L.J.4
  • 24
    • 24344461752 scopus 로고    scopus 로고
    • Culture techniques for soil dwelling earthworms: A review
    • Lowe, C.N. & Butt, K.R. (2005) Culture techniques for soil dwelling earthworms: A review. Pedobiologia 49, 401-413.
    • (2005) Pedobiologia , vol.49 , pp. 401-413
    • Lowe, C.N.1    Butt, K.R.2
  • 26
    • 18944378710 scopus 로고    scopus 로고
    • The influence of mineral and organic fertilisers on the growth of the endogeic earthworm Octolasion tyrtaeum (Savigny)
    • Marhan, S. & Scheu, S. (2005) The influence of mineral and organic fertilisers on the growth of the endogeic earthworm Octolasion tyrtaeum (Savigny). Pedobiologia 49, 239-249.
    • (2005) Pedobiologia , vol.49 , pp. 239-249
    • Marhan, S.1    Scheu, S.2
  • 27
    • 0347930404 scopus 로고    scopus 로고
    • A microscopical view of the intestine of Onychochaeta borincana (Oligochaeta: Glossoscolecidae)
    • Mendez, R., Borges, S. & Betancourt, C. (2003) A microscopical view of the intestine of Onychochaeta borincana (Oligochaeta: Glossoscolecidae). Pedobiologia 47, 900-903.
    • (2003) Pedobiologia , vol.47 , pp. 900-903
    • Mendez, R.1    Borges, S.2    Betancourt, C.3
  • 28
    • 0036890004 scopus 로고    scopus 로고
    • New PCR primers for the selective amplification of 16S rDNA from different groups of actinomycetes
    • Monciardini, P., Sossio, M., Cavaletti, L., Chiocchini, C. & Donadio, S. (2002) New PCR primers for the selective amplification of 16S rDNA from different groups of actinomycetes. FEMS Microbiology Ecology 42, 419-429.
    • (2002) FEMS Microbiology Ecology , vol.42 , pp. 419-429
    • Monciardini, P.1    Sossio, M.2    Cavaletti, L.3    Chiocchini, C.4    Donadio, S.5
  • 29
    • 0031959006 scopus 로고    scopus 로고
    • Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology
    • Muyzer, G. & Smalla, K. (1998) Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie van Leeuwenhoek 73, 127-141.
    • (1998) Antonie van Leeuwenhoek , vol.73 , pp. 127-141
    • Muyzer, G.1    Smalla, K.2
  • 30
    • 0027460328 scopus 로고
    • Profiling of complex microbial populations by denaturing gradient gel electrophoresis analyses of polymerase chain reaction-amplified genes for 16S rRNA
    • Muyzer, G., de Waal, E.C. & Uitterlinden, A.G. (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analyses of polymerase chain reaction-amplified genes for 16S rRNA. Applied and Environmental Microbiology 59, 695-700.
    • (1993) Applied and Environmental Microbiology , vol.59 , pp. 695-700
    • Muyzer, G.1    de Waal, E.C.2    Uitterlinden, A.G.3
  • 31
    • 0027829996 scopus 로고
    • Effect of passage through the intestinal-tract of detritivore earthworms (Lumbricus spp.) on the number of selected Gram-negative and total bacteria
    • Pedersen, J.C. & Hendriksen, N.B. (1993) Effect of passage through the intestinal-tract of detritivore earthworms (Lumbricus spp.) on the number of selected Gram-negative and total bacteria. Biology and Fertility of Soils 16, 227-232.
    • (1993) Biology and Fertility of Soils , vol.16 , pp. 227-232
    • Pedersen, J.C.1    Hendriksen, N.B.2
  • 33
    • 0037319936 scopus 로고    scopus 로고
    • Application of 16S rDNA-DGGE to examine the microbial ecology associated with a social wasp Vespula germanica
    • Reeson, A.F., Jankovic, T., Kasper, M.L., Rogers, S. & Austin, A.D. (2003) Application of 16S rDNA-DGGE to examine the microbial ecology associated with a social wasp Vespula germanica. Insect Molecular Biology 12, 85-91.
    • (2003) Insect Molecular Biology , vol.12 , pp. 85-91
    • Reeson, A.F.1    Jankovic, T.2    Kasper, M.L.3    Rogers, S.4    Austin, A.D.5
  • 34
    • 33750824832 scopus 로고    scopus 로고
    • Changes in the fatty acid profiles through the digestive tract of the earthworm Lumbricus terrestris L
    • Sampedro, L. & Whalen, J.K. (2007) Changes in the fatty acid profiles through the digestive tract of the earthworm Lumbricus terrestris L. Applied Soil Ecology 35, 226-236.
    • (2007) Applied Soil Ecology , vol.35 , pp. 226-236
    • Sampedro, L.1    Whalen, J.K.2
  • 35
    • 0028116158 scopus 로고
    • Rapid silver staining and recovery for PCR products separated on polyacrylamide gels
    • Sanguinetti, C.J., Net, E.D. & Simpson, A.J.G. (1994) Rapid silver staining and recovery for PCR products separated on polyacrylamide gels. BioTechniques 17, 915-919.
    • (1994) BioTechniques , vol.17 , pp. 915-919
    • Sanguinetti, C.J.1    Net, E.D.2    Simpson, A.J.G.3
  • 37
    • 0036132828 scopus 로고    scopus 로고
    • Automated image analysis and in situ hybridization as tools to study bacterial populations in food resources, gut and cast of Lumbricus terrestris L
    • Schönholzer, F., Hahn, D., Zarda, B. & Zeyer, J. (2002) Automated image analysis and in situ hybridization as tools to study bacterial populations in food resources, gut and cast of Lumbricus terrestris L. Journal of Microbiological Methods 48, 53-68.
    • (2002) Journal of Microbiological Methods , vol.48 , pp. 53-68
    • Schönholzer, F.1    Hahn, D.2    Zarda, B.3    Zeyer, J.4
  • 38
    • 0031764376 scopus 로고    scopus 로고
    • A new approach to utilize PCR-single-strand conformation polymorphism for 16S rRNA gene-based microbial community analysis
    • Schwieger, F. & Tebbe, C.C. (1998) A new approach to utilize PCR-single-strand conformation polymorphism for 16S rRNA gene-based microbial community analysis. Applied and Environmental Microbiology 64, 4870-4876.
    • (1998) Applied and Environmental Microbiology , vol.64 , pp. 4870-4876
    • Schwieger, F.1    Tebbe, C.C.2
  • 39
    • 0032925262 scopus 로고    scopus 로고
    • Application of denaturant gradient gel electrophoresis for the analysis of the porcine gastrointestinal microbiota
    • Simpson, J.M., McCracken, V.J., White, B.A., Gaskins, H.R. & Mackie, R.I. (1999) Application of denaturant gradient gel electrophoresis for the analysis of the porcine gastrointestinal microbiota. Journal of Microbiological Methods 36, 167-179.
    • (1999) Journal of Microbiological Methods , vol.36 , pp. 167-179
    • Simpson, J.M.1    McCracken, V.J.2    White, B.A.3    Gaskins, H.R.4    Mackie, R.I.5
  • 40
    • 0242659175 scopus 로고    scopus 로고
    • Identification of uncultured bacteria tightly associated with the intestine of the earthworm Lumbricus rubellus (Lumbricidae, Oligochaeta)
    • Singleton, D.R., Hendrix, P.F., Coleman, D.C. & Whitman, W.B. (2003) Identification of uncultured bacteria tightly associated with the intestine of the earthworm Lumbricus rubellus (Lumbricidae, Oligochaeta). Soil Biology and Biochemistry 35, 1547-1555.
    • (2003) Soil Biology and Biochemistry , vol.35 , pp. 1547-1555
    • Singleton, D.R.1    Hendrix, P.F.2    Coleman, D.C.3    Whitman, W.B.4
  • 41
    • 0030067991 scopus 로고    scopus 로고
    • Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR
    • Suzuki, M. & Giovannoni, S. (1996) Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR. Applied and Environmental Microbiology 62, 625-630.
    • (1996) Applied and Environmental Microbiology , vol.62 , pp. 625-630
    • Suzuki, M.1    Giovannoni, S.2
  • 43
    • 0345195995 scopus 로고    scopus 로고
    • V. Wintzingerode, F., Göbel, U.B. & Stackebrandt, E. (1997) Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis. FEMS Microbiology Reviews 21, 213-229.
    • V. Wintzingerode, F., Göbel, U.B. & Stackebrandt, E. (1997) Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis. FEMS Microbiology Reviews 21, 213-229.
  • 44
    • 0033824163 scopus 로고    scopus 로고
    • Direct analysis of wood-inhabiting fungi using denaturing gradient gel electrophoresis of amplified ribosomal DNA
    • Vainio, E.J. & Hantula, J. (2000) Direct analysis of wood-inhabiting fungi using denaturing gradient gel electrophoresis of amplified ribosomal DNA. Mycological Research 104, 927-936.
    • (2000) Mycological Research , vol.104 , pp. 927-936
    • Vainio, E.J.1    Hantula, J.2
  • 45
    • 2442616728 scopus 로고    scopus 로고
    • Temporal stability analysis of the microbiota in human faeces by denaturing gradient gel electrophoresis using universal and group-specific 16S rRNA gene primers
    • Vanhoutte, T., Huys, G., De Brandt, E. & Swings, J. (2004) Temporal stability analysis of the microbiota in human faeces by denaturing gradient gel electrophoresis using universal and group-specific 16S rRNA gene primers. FEMS Microbiology Ecology 48, 437-446.
    • (2004) FEMS Microbiology Ecology , vol.48 , pp. 437-446
    • Vanhoutte, T.1    Huys, G.2    De Brandt, E.3    Swings, J.4
  • 46
    • 84944178665 scopus 로고
    • Hierarchical grouping to optimize an objective function
    • Ward, J.H. (1963) Hierarchical grouping to optimize an objective function. Journal of the American Statistical Association 58, 236-244.
    • (1963) Journal of the American Statistical Association , vol.58 , pp. 236-244
    • Ward, J.H.1
  • 47
    • 0034450921 scopus 로고    scopus 로고
    • Molecular detection of bacterial and streptomycete chitinases in the environment
    • Williamson, N., Brian, P. & Wellington, E.M.H. (2000) Molecular detection of bacterial and streptomycete chitinases in the environment. Antonie van Leeuwenhoek 78, 315-321.
    • (2000) Antonie van Leeuwenhoek , vol.78 , pp. 315-321
    • Williamson, N.1    Brian, P.2    Wellington, E.M.H.3


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