Huntingtin triplet-repeat locus is stable under long-term Fen1 knockdown in human cells

Journal of Neuroscience Methods

Volumn 171, Issue 2, 2008, Pages 233-238

  Moe, Svein Erik ^a   Sorbo, Jan Gunnar ^a   Holen, Torgeir ^a  

^a UNIVERSITY OF OSLO   (Norway)

Author keywords

Fen1; Huntingtin; PCR; RNAi

Indexed keywords

FLAP ENDONUCLEASE; FLAP ENDONUCLEASE 1; HUNTINGTIN; PROTEIN MUTS;

ARTICLE; CELL LINE; CONTROLLED STUDY; DNA REPAIR; DROSOPHILA; GENE LOCUS; HUMAN; HUMAN CELL; MOUSE; MYOTONIC DYSTROPHY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA INTERFERENCE;

CELL LINE, TRANSFORMED; CLONING, MOLECULAR; COMPUTATIONAL BIOLOGY; FLAP ENDONUCLEASES; HUMANS; NERVE TISSUE PROTEINS; NUCLEAR PROTEINS; RNA INTERFERENCE; TIME FACTORS; TRINUCLEOTIDE REPEAT EXPANSION;

EID: 43449125903     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2008.03.012     Document Type: Article

References (29)

1. Bates G. Huntingtin aggregation and toxicity in Huntington's disease. Lancet 361 (2003) 1642-1644
2. Cha J.H. Transcriptional dysregulation in Huntington's disease. Trends Neurosci 23 (2000) 387-392
3. Freudenreich C.H., Kantrow S.M., and Zakian V.A. Expansion and length-dependent fragility of CTG repeats in yeast. Science 279 (1998) 853-856
4. Gordenin D.A., Kunkel T.A., and Resnick M.A. Repeat expansion-all in a flap?. Nat Genet 16 (1997) 116-118
5. Gusella J.F., and Macdonald M.E. Huntington's disease: seeing the pathogenic process through a genetic lens. Trends Biochem Sci 31 (2006) 533-540
6. Harrington J.J., and Lieber M.R. The characterization of a mammalian DNA structure-specific endonuclease. EMBO J 13 (1994) 1235-1246
7. Holen T. Efficient prediction of siRNAs with siRNArules 1.0: an open-source JAVA approach to siRNA algorithms. RNA 12 (2006) 1620-1625
8. Holen T., Amarzguioui M., Wiiger M.T., Babaie E., and Prydz H. Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor. Nucleic Acids Res 30 (2002) 1757-1766
9. Holen T., Moe S.E., Sorbo J.G., Meza T.J., Ottersen O.P., and Klungland A. Tolerated wobble mutations in siRNAs decrease specificity, but can enhance activity in vivo. Nucleic Acids Res 33 (2005) 4704-4710
10. Hunter J.M., Crouse A.B., Lesort M., Johnson G.V., and Detloff P.J. Verification of somatic CAG repeat expansion by pre-PCR fractionation. J Neurosci Methods 144 (2005) 11-17
11. Jackson S.M., Whitworth A.J., Greene J.C., Libby R.T., Baccam S.L., Pallanck L.J., et al. A SCA7 CAG/CTG repeat expansion is stable in Drosophila melanogaster despite modulation of genomic context and gene dosage. Gene 347 (2005) 35-41
12. Landles C., and Bates G.P. Huntingtin and the molecular pathogenesis of Huntington's disease. Fourth in molecular medicine review series. EMBO Rep 5 (2004) 958-963
13. Leeflang E.P., Zhang L., Tavare S., Hubert R., Srinidhi J., Macdonald M.E., et al. Single sperm analysis of the trinucleotide repeats in the Huntington's disease gene: quantification of the mutation frequency spectrum. Hum Mol Genet 4 (1995) 1519-1526
14. Matsukura S., Jones P.A., and Takai D. Establishment of conditional vectors for hairpin siRNA knockdowns. Nucleic Acids Res 31 (2003) e77
15. 2. Nucleic Acids Res 30 (2002) 3273-3277
16. Moore H., Greenwell P.W., Liu C.P., Arnheim N., and Petes T.D. Triplet repeats form secondary structures that escape DNA repair in yeast. Proc Natl Acad Sci USA 96 (1999) 1504-1509
17. Otto C.J., Almqvist E., Hayden M.R., and Andrew S.E. The "flap" endonuclease gene FEN1 is excluded as a candidate gene implicated in the CAG repeat expansion underlying Huntington disease. Clin Genet 59 (2001) 122-127
18. Parniewski P., and Staczek P. Molecular mechanisms of TRS instability. Adv Exp Med Biol 516 (2002) 1-25
19. Ravikumar B., Vacher C., Berger Z., Davies J.E., Luo S., Oroz L.G., et al. Inhibition of mTOR induces autophagy and reduces toxicity of polyglutamine expansions in fly and mouse models of Huntington disease. Nat Genet 36 (2004) 585-595
20. Schwarz D.S., Hutvagner G., Du T., Xu Z., Aronin N., and Zamore P.D. Asymmetry in the assembly of the RNAi enzyme complex. Cell 115 (2003) 199-208
21. Siolas D., Lerner C., Burchard J., Ge W., Linsley P.S., Paddison P.J., et al. Synthetic shRNAs as potent RNAi triggers. Nat Biotechnol 23 (2005) 227-231
22. Song J.J., Liu J., Tolia N.H., Schneiderman J., Smith S.K., Martienssen R.A., et al. The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes. Nat Struct Biol 10 (2003) 1026-1032
23. Spiro C., and McMurray C.T. Nuclease-deficient FEN-1 blocks Rad51/BRCA1-mediated repair and causes trinucleotide repeat instability. Mol Cell Biol 23 (2003) 6063-6074
24. ter Brake O., Konstantinova P., Ceylan M., and Berkhout B. Silencing of HIV-1 with RNA interference: a multiple shRNA approach. Mol Ther 14 (2006) 883-892
25. The Huntington's Disease Collaborative Research Group. A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes. Cell 72 (1993) 971-983
26. Tishkoff D.X., Filosi N., Gaida G.M., and Kolodner R.D. A novel mutation avoidance mechanism dependent on S. cerevisiae RAD27 is distinct from DNA mismatch repair. Cell 88 (1997) 253-263
27. van den Broek W.J., Nelen M.R., van der Heijden G.W., Wansink D.G., and Wieringa B. Fen1 does not control somatic hypermutability of the (CTG)(n)*(CAG)(n) repeat in a knock-in mouse model for DM1. FEBS Lett 580 (2006) 5208-5214
28. Vermeulen A., Behlen L., Reynolds A., Wolfson A., Marshall W.S., Karpilow J., et al. The contributions of dsRNA structure to Dicer specificity and efficiency. RNA 11 (2005) 674-682
29. Yang J., and Freudenreich C.H. Haploinsufficiency of yeast FEN1 causes instability of expanded CAG/CTG tracts in a length-dependent manner. Gene 393 (2007) 110-115