Broad-host-range cloning and cassette vectors based on the R388 trimethoprim resistance gene

BioTechniques

Volumn 20, Issue 5, 1996, Pages 762-764

  DeShazer, David ^a   Woods, Donald E ^a  

^a Health Sciences Centre Calgary   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

TRIMETHOPRIM;

ANTIBIOTIC RESISTANCE; CONTROLLED STUDY; EXPRESSION VECTOR; MOLECULAR CLONING; NONHUMAN; PLASMID; SHORT SURVEY;

ANIMALS; BACTERIA; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA, BACTERIAL; GENETIC MARKERS; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; PLASMIDS; POLYMERASE CHAIN REACTION; TRIMETHOPRIM RESISTANCE;

EID: 0029899029     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96205bm05     Document Type: Article

References (6)

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2. Kovach, M.E., R.W. Phillips, P.H. Elzer, R.M. Roop II and K.M. Peterson. 1994. pBBRIMCS: a broad-host-range cloning vector. BioTechniques 16:801-802.
3. Levesque, C., S. Brassard, J. Lapointe and P.H. Roy. 1994. Diversity and relative strength of tandem promoters for the antibiotic-resistance genes of several integrons. Gene 142:49-54.
4. Mullis, K.B. and F.A. Faloona. 1987. Specific synthesis of DNA in vitro via a polymerasecatalyzed chain reaction. Methods Enzymol. 155:335-350.
5. Tsang, T., V. Copeland and G.T. Bowden. 1991. A set of cassette cloning vectors for rapid and versatile adaption of restriction fragments. BioTechniques 10:330.
6. Zolg, J.W. and U.J. Hanggi. 1981. Characterization of an R plasmid-associated, trimethoprim-resistant dihydrofolate reductase and determination of the nucleotide sequence of the reductase gene. Nucleic Acids Res. 9:697-710.