Multiplexed real-time PCR using universal reporters

Clinical Chemistry

Volumn 50, Issue 9, 2004, Pages 1680-1683

  Rickert, Andreas M ^a   Lehrach, Hans ^a   Sperling, Silke ^a  

^a MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; COST BENEFIT ANALYSIS; DNA HYBRIDIZATION; GENE AMPLIFICATION; GENE EXPRESSION; GENE LOCUS; GENE SEQUENCE; GENE TARGETING; INTERMETHOD COMPARISON; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; UPREGULATION;

DNA PRIMERS; GENES, REPORTER; HUMANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 4344665679     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2004.034512     Document Type: Article

References (20)

1. Giulietti A, Overbergh L, Valckx D, Decallonne B, Bouillon R, Mathieu C. An overview of real-time quantitative PCR: applications to quantify cytokine gene expression. Methods 2001;25:386-401.
2. Higuchi R, Dollinger G, Walsh PS, Griffith R. Simultaneous amplification and detection of specific DNA sequences. Biotechnology 1992;10:413-7.
3. Nielsen PE. Sequence-selective DNA recognition by synthetic ligands. Bioconj Chem 1991;2:1-12.
4. Morrison TB, Weis JJ, Wittwer CT. Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification. Biotechniques 1998;24:954-8, 960, 962.
5. Tyagi S, Bratu DP, Kramer FR. Multicolor molecular beacons for allele discrimination. Nat Biotechnol 1998;16:49-53.
6. Nazarenko IA, Bhatnagar SK, Hohman RJ. A closed tube format for amplification and detection of DNA based on energy transfer. Nucleic Acids Res 1997;25:2516-21.
7. Whitcombe D, Theaker J, Guy SP, Brown T, Little S. Detection of PCR products using self-probing amplicons and fluorescence. Nat Biotechnol 1999;17:804-7.
8. Holland PM, Abramson RD, Watson R, Gelfand DH. Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci U S A 1991;88:7276-80.
9. Livak KJ, Flood SJ, Marmaro J, Giusti W, Deetz K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl 1995;4:357-62.
10. Moser MJ, Marshall DJ, Grennier JK, Kiefer CD, Killeen AA, Ptacin JL, et al. Exploiting the enzymatic recognition of an unnatural base pair to develop a universal genetic analysis system. Clin Chem 2003;49:407-14.
11. Nazarenko I, Lowe B, Darfler M, Ikonomi P, Schuster D, Rashtchian A. Multiplexed quantitative PCR using self-quenched primers labeled with single fluorophore. Nucleic Acid Res 2002;e37.
12. Crockett AO, Wittwer CT. Fluorescein-labeled oligonucleotides for real-time PCR: using the inherent quenching of deoxyguanosine nucleotides. Anal Biochem 2001;290:89-97.
13. Svanvik N, Stahlberg A, Sehlstedt U, Sjöback R, Kubista M. Detection of PCR products in real time using light-up probes. Anal Biochem 2000;287:179-82.
14. Myakishev MV, Khripin Y, Hu S, Hamer DH. High-throughput SNP genotyping by allele-specific PCR with universal energy-transfer-labeled primers. Genome Res 2001;11:163-9.
15. Whitcombe D, Brownie J, Gillard HL, McKechnie D, Theaker J, Newton CR, et al. A homogeneous fluorescence assay for PCR amplicons: its application to real-time, single-tube genotyping. Clin. Chem 1998;44:918-23.
16. Nuovo GJ, Hohman RJ, Nardone GA, Nazarenko IA. In situ amplification using universal energy transfer-labeled primers. J Histochem Cytochem 1999;47:273-80.
17. Kaynak B, von Heydebreck A, Mebus S, Seelow D, Hennig S, Vogel J, et al. Genome-wide array analysis of normal and malformed human hearts. Circulation 2003;107:2467-74.
18. Borodina TA, Lehrach H, Soldatov AV. Ligation-based synthesis of oligonucleotides with block structure. Anal Biochem 2003;318:309-13.
19. Mikula M, Dzwonek A, Jagusztyn-Krynicka K, Ostrowski J. Quantitative detection for low levels of Helicobacter pylori infection in experimentally infected mice by real-time PCR. J Microbiol Methods 2003;55:351-9.
20. Ponchel F, Toomes C, Bransfield K, Leong FT, Douglas SH, Field SL, et al. Real-time PCR based on SYBR-Green I fluorescence: an alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions. BMC Biotechnol 2003;3:18.