The small molecule Mek1/2 inhibitor U0126 disrupts the chordamesoderm to notochord transition in zebrafish

BMC Developmental Biology

Volumn 8, Issue , 2008, Pages

  Hawkins, Thomas A ^a   Cavodeassi, Florencia ^a   Erdélyi, Ferenc ^b   Szabó, Gábor ^b   Lele, Zsolt ^a,b  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

[No Author keywords available]

Indexed keywords

1,4 DIAMINO 1,4 BIS(2 AMINOPHENYLTHIO) 2,3 DICYANOBUTADIENE; FIBROBLAST GROWTH FACTOR; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE KINASE 2; 1,3 BUTADIENE DERIVATIVE; ENZYME INHIBITOR; NITRILE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DEVELOPMENTAL STAGE; ELECTRON MICROSCOPY; EMBRYO; ENZYME INHIBITION; GASTRULATION; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; IN VIVO STUDY; MESODERM; NICK END LABELING; NONHUMAN; NOTOCHORD; PHENOTYPE; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; SOMITE; ZEBRA FISH; ANIMAL; ANIMAL EMBRYO; APOPTOSIS; BASEMENT MEMBRANE; CYTOSKELETON; DOSE RESPONSE; DRUG ANTAGONISM; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; PRENATAL DEVELOPMENT;

ANIMALIA; DANIO RERIO; MUS;

ANIMALS; APOPTOSIS; BASEMENT MEMBRANE; BUTADIENES; CYTOSKELETON; DOSE-RESPONSE RELATIONSHIP, DRUG; EMBRYO, NONMAMMALIAN; ENZYME INHIBITORS; FIBROBLAST GROWTH FACTORS; GASTRULATION; GENE EXPRESSION REGULATION, DEVELOPMENTAL; MAP KINASE KINASE 1; MAP KINASE KINASE 2; MESODERM; NITRILES; NOTOCHORD; PHENOTYPE; ZEBRAFISH;

EID: 43249121573     PISSN: None     EISSN: 1471213X     Source Type: Journal    
DOI: 10.1186/1471-213X-8-42     Document Type: Article

References (80)

1. The zebrafish as a model system in developmental, toxicological and transgenic research. Z Lele PH Krone, Biotechnology Advances 1996 14 1 57 72 10.1016/0734-9750(96)00004-3 14536924
2. Hedgehog and Fgf signaling pathways regulate the development of tphR-expressing serotonergic raphe neurons in zebrafish embryos. H Teraoka C Russell J Regan A Chandrasekhar ML Concha R Yokoyama K Higashi M Take-Uchi W Dong T Hiraga N Holder SW Wilson, J Neurobiol 2004 60 3 275 288 10.1002/neu.20023 15281067
3. Analysis of the zebrafish smoothened mutant reveals conserved and divergent functions of hedgehog activity. W Chen S Burgess N Hopkins, Development 2001 128 12 2385 2396 11493557
4. Interplay between FGF, one-eyed pinhead, and T-box transcription factors during zebrafish posterior development. KJ Griffin D Kimelman, Dev Biol 2003 264 2 456 466 10.1016/j.ydbio.2003.09.008 14651930
5. Specification of dorsal telencephalic character by sequential Wnt and FGF signaling. L Gunhaga M Marklund M Sjodal JC Hsieh TM Jessell T Edlund, Nat Neurosci 2003 6 7 701 707 10.1038/nn1068 12766771
6. Time-dependent patterning of the mesoderm and endoderm by Nodal signals in zebrafish. EG Hagos ST Dougan, BMC Dev Biol 2007 7 22 17391517 10.1186/1471-213X-7-22
7. Biochemical suppression of small-molecule inhibitors: a strategy to identify inhibitor targets and signaling pathway components. JR Peterson AM Lebensohn HE Pelish MW Kirschner, Chem Biol 2006 13 4 443 452 16632257 10.1016/j.chembiol.2006.02.009
8. In vivo drug discovery in the zebrafish. LI Zon RT Peterson, Nature reviews 2005 4 1 35 44 10.1038/nrd1606 15688071
9. Identification of a novel inhibitor of mitogen-activated protein kinase kinase. MF Favata KY Horiuchi EJ Manos AJ Daulerio DA Stradley WS Feeser DE Van Dyk WJ Pitts RA Earl F Hobbs RA Copeland RL Magolda PA Scherle JM Trzaskos, J Biol Chem 1998 273 29 18623 18632 10.1074/jbc.273.29.18623 9660836
10. Organization and regulation of mitogen-activated protein kinase signaling pathways. TP Garrington GL Johnson, Curr Opin Cell Biol 1999 11 2 211 218 10.1016/S0955-0674(99)80028-3 10209154
11. p38 MAP kinases: beyond the stress response. AR Nebreda A Porras, Trends Biochem Sci 2000 25 6 257 260 10.1016/S0968-0004(00)01595-4 10838561
12. The JNK signal transduction pathway. CR Weston RJ Davis, Curr Opin Genet Dev 2002 12 1 14 21 10.1016/S0959-437X(01)00258-1 11790549
13. ERK and p38 MAPK-activated protein kinases: a family of protein kinases with diverse biological functions. PP Roux J Blenis, Microbiol Mol Biol Rev 2004 68 2 320 344 15187187 10.1128/MMBR.68.2.320-344.2004
14. Functions of the MAPK family in vertebrate-development. SF Krens HP Spaink BE Snaar-Jagalska, FEBS Lett 2006 580 21 4984 4990 10.1016/j.febslet. 2006.08.025 16949582
15. Distinct functions for ERKs? AC Lloyd, J Biol 2006 5 5 13 16879721 10.1186/jbiol46
16. MAPK signalling: ERK5 versus ERK1/2. S Nishimoto E Nishida, EMBO Rep 2006 7 8 782 786 16880823 10.1038/sj.embor.7400755
17. Characterization and expression patterns of the MAPK family in zebrafish. SF Krens S He HP Spaink BE Snaar-Jagalska, Gene Expr Patterns 2006 6 8 1019 1026 10.1016/j.modgep.2006.04.008 16774848
18. MEK inhibitors: the chemistry and biological activity of U0126, its analogs, and cyclization products. JV Duncia JB Santella 3rd CA Higley WJ Pitts J Wityak WE Frietze FW Rankin JH Sun RA Earl AC Tabaka CA Teleha KF Blom MF Favata EJ Manos AJ Daulerio DA Stradley K Horiuchi RA Copeland PA Scherle JM Trzaskos RL Magolda GL Trainor RR Wexler FW Hobbs RE Olson, Bioorg Med Chem Lett 1998 8 20 2839 2844 10.1016/S0960-894X(98)00522-8 9873633
19. Role of endothelial cell extracellular signal-regulated kinase1/2 in urokinase-type plasminogen activator upregulation and in vitro angiogenesis by fibroblast growth factor-2. R Giuliani M Bastaki D Coltrini M Presta, J Cell Sci 1999 112 ( Pt 15) 2597 2606 10393815
20. Angiotensin II induces endothelin-1 gene expression via extracellular signal-regulated kinase pathway in rat aortic smooth muscle cells. HJ Hong P Chan JC Liu SH Juan MT Huang JG Lin TH Cheng, Cardiovasc Res 2004 61 1 159 168 10.1016/j.cardiores.2003.10.019 14732213
21. ERK and p38 MAP kinase are required for rat renal development. M Hida S Omori M Awazu, Kidney Int 2002 61 4 1252 1262 10.1046/j.1523-1755.2002.00273.x 11918731
22. Activated extracellular signal-regulated kinases are necessary and sufficient to initiate tubulogenesis in renal tubular MDCK strain I cell cysts. NE Hellman AJ Greco KK Rogers C Kanchagar DF Balkovetz JH Lipschutz, Am J Physiol Renal Physiol 2005 289 4 F777 85 10.1152/ajprenal.00429.2004 15900019
23. Control of the segmentation process by graded MAPK/ERK activation in the chick embryo. MC Delfini J Dubrulle P Malapert J Chal O Pourquie, Proc Natl Acad Sci U S A 2005 102 32 11343 11348 16055560 10.1073/pnas.0502933102
24. Studies of the mitogen-activated protein kinases and phosphatidylinositol-3 kinase in the lens. 1. The mitogenic and stress responses. SD Zatechka Jr. MF Lou, Exp Eye Res 2002 74 6 703 717 10.1006/exer.2002.1168 12126944
25. Multiple signaling pathways regulate FGF-2-induced retinal ganglion cell neurite extension and growth cone guidance. CA Webber YY Chen CL Hehr J Johnston S McFarlane, Mol Cell Neurosci 2005 30 1 37 47 10.1016/j.mcn.2005.05.005 15996482
26. ERK signaling is required for eye-specific retino-geniculate segregation. S Naska MC Cenni E Menna L Maffei, Development 2004 131 15 3559 3570 10.1242/dev.01212 15215205
27. A conserved role for the MEK signalling pathway in neural tissue specification and posteriorisation in the invertebrate chordate, the ascidian Ciona intestinalis. C Hudson S Darras D Caillol H Yasuo P Lemaire, Development 2003 130 1 147 159 10.1242/dev.00200 12441299
28. Role of the FGF and MEK signaling pathway in the ascidian embryo. GJ Kim H Nishida, Dev Growth Differ 2001 43 5 521 533 10.1046/j.1440-169X.2001.00594.x 11576169
29. Binary specification of nerve cord and notochord cell fates in ascidian embryos. T Minokawa K Yagi KW Makabe H Nishida, Development 2001 128 11 2007 2017 11493523
30. Structure and function of the notochord: an essential organ for chordate development. DL Stemple, Development 2005 132 11 2503 2512 10.1242/dev.01812 15890825
31. Up-regulation of the chondrogenic Sox9 gene by fibroblast growth factors is mediated by the mitogen-activated protein kinase pathway 10.1073/pnas.97.3. 1113. S Murakami M Kan WL McKeehan B de Crombrugghe, Proceedings of the National Academy of Sciences 2000 97 3 1113 1118 10.1073/pnas.97.3.1113 10655493
32. Topical Treatment with Inhibitors of the Phosphatidylinositol 3'-Kinase/Akt and Raf/Mitogen-Activated Protein Kinase Kinase/Extracellular Signal-Regulated Kinase Pathways Reduces Melanoma Development in Severe Combined Immunodeficient Mice 10.1158/0008-5472.CAN-03-3327. B Bedogni MS O'Neill SM Welford DM Bouley AJ Giaccia NC Denko MB Powell, Cancer Res 2004 64 7 2552 2560 10.1158/0008-5472.CAN-03-3327 15059911
33. Artery/vein specification is governed by opposing phosphatidylinositol-3 kinase and MAP kinase/ERK signaling. CC Hong QP Peterson JY Hong RT Peterson, Curr Biol 2006 16 13 1366 1372 16824925 10.1016/j.cub.2006.05.046
34. A Synthetic Inhibitor of the Mitogen-Activated Protein Kinase Cascade 10.1073/pnas.92.17.7686. DT Dudley L Pang SJ Decker AJ Bridges AR Saltiel, Proceedings of the National Academy of Sciences 1995 92 17 7686 7689 10.1073/pnas.92.17.7686 7644477
35. Regulation of bidirectional melanosome transport by organelle bound MAP kinase. SW Deacon A Nascimento AS Serpinskaya VI Gelfand, Curr Biol 2005 15 5 459 463 10.1016/j.cub.2004.12.074 15753041
36. A role for FGF-8 in the dorsoventral patterning of the zebrafish gastrula. M Furthauer C Thisse B Thisse, Development 1997 124 21 4253 4264 9334274
37. sprouty4 acts in vivo as a feedback-induced antagonist of FGF signaling in zebrafish. M Furthauer F Reifers M Brand B Thisse C Thisse, Development 2001 128 12 2175 2186 11493538
38. Sef is a feedback-induced antagonist of Ras/MAPK-mediated FGF signalling. M Furthauer W Lin SL Ang B Thisse C Thisse, Nat Cell Biol 2002 4 2 170 174 10.1038/ncb750 11802165
39. Fgf signalling controls the dorsoventral patterning of the zebrafish embryo. M Furthauer J Van Celst C Thisse B Thisse, Development 2004 131 12 2853 2864 10.1242/dev.01156 15151985
40. Genetic analysis of dorsoventral pattern formation in the zebrafish: requirement of a BMP-like ventralizing activity and its dorsal repressor. M Hammerschmidt GN Serbedzija AP McMahon, Genes Dev 1996 10 19 2452 2461 10.1101/gad.10.19.2452 8843197
41. Genes establishing dorsoventral pattern formation in the zebrafish embryo: the ventral specifying genes. MC Mullins M Hammerschmidt DA Kane J Odenthal M Brand FJ van Eeden M Furutani-Seiki M Granato P Haffter CP Heisenberg YJ Jiang RN Kelsh C Nusslein-Volhard, Development 1996 123 81 93 9007231
42. Mutations affecting cell fates and cellular rearrangements during gastrulation in zebrafish. L Solnica-Krezel DL Stemple E Mountcastle-Shah Z Rangini SC Neuhauss J Malicki AF Schier DY Stainier F Zwartkruis S Abdelilah W Driever, Development 1996 123 67 80 9007230
43. Functional interactions of genes mediating convergent extension, knypek and trilobite, during the partitioning of the eye primordium in zebrafish. F Marlow F Zwartkruis J Malicki SC Neuhauss L Abbas M Weaver W Driever L Solnica-Krezel, Dev Biol 1998 203 2 382 399 10.1006/dbio.1998.9032 9808788
44. The zebrafish glypican knypek controls cell polarity during gastrulation movements of convergent extension. J Topczewski DS Sepich DC Myers C Walker A Amores Z Lele M Hammerschmidt J Postlethwait L Solnica-Krezel, Dev Cell 2001 1 2 251 264 10.1016/S1534-5807(01)00005-3 11702784
45. Sonic hedgehog, secreted by amacrine cells, acts as a short-range signal to direct differentiation and lamination in the zebrafish retina 10.1242/dev.01247. A Shkumatava S Fischer F Muller U Strahle CJ Neumann, Development 2004 131 16 3849 3858 10.1242/dev.01247 15253932
46. no tail (ntl) is the zebrafish homologue of the mouse T (Brachyury) gene. S Schulte-Merker FJ van Eeden ME Halpern CB Kimmel C Nusslein-Volhard, Development 1994 120 4 1009 1015 7600949
47. Induction of muscle pioneers and floor plate is distinguished by the zebrafish no tail mutation. ME Halpern RK Ho C Walker CB Kimmel, Cell 1993 75 1 99 111 8402905
48. Action of the Brachyury gene in mouse embryogenesis. BG Herrmann, Ciba Found Symp 1992 165 78 86; discussion 86-91 1516477
49. Expression pattern of the mouse T gene and its role in mesoderm formation. DG Wilkinson S Bhatt BG Herrmann, Nature 1990 343 6259 657 659 10.1038/343657a0 1689462
50. Immunohistochemical analysis of the Brachyury protein in wild-type and mutant mouse embryos. A Kispert BG Herrmann, Dev Biol 1994 161 1 179 193 10.1006/dbio.1994.1019 8293872
51. Zebrafish mutants identify an essential role for laminins in notochord formation. MJ Parsons SM Pollard L Saude B Feldman P Coutinho EM Hirst DL Stemple, Development 2002 129 13 3137 3146 12070089
52. Essential and overlapping roles for laminin alpha chains in notochord and blood vessel formation. SM Pollard MJ Parsons M Kamei RN Kettleborough KA Thomas VN Pham MK Bae A Scott BM Weinstein DL Stemple, Dev Biol 2006 289 1 64 76 10.1016/j.ydbio.2005.10.006 16321372
53. Fgf signalling through MAPK cascade is required for development of the subpallial telencephalon in zebrafish embryos. M Shinya S Koshida A Sawada A Kuroiwa H Takeda, Development 2001 128 21 4153 4164 11684653
54. Differential requirements for COPI transport during vertebrate early development. P Coutinho MJ Parsons KA Thomas EM Hirst L Saude I Campos PH Williams DL Stemple, Dev Cell 2004 7 4 547 558 10.1016/j.devcel.2004.07.020 15469843
55. Atp7a determines a hierarchy of copper metabolism essential for notochord development. BA Mendelsohn C Yin SL Johnson TP Wilm L Solnica-Krezel JD Gitlin, Cell Metab 2006 4 2 155 162 10.1016/j.cmet.2006.05.001 16890543
56. Embryonic death of Mek1-deficient mice reveals a role for this kinase in angiogenesis in the labyrinthine region of the placenta. S Giroux M Tremblay D Bernard JF Cardin-Girard S Aubry L Larouche S Rousseau J Huot J Landry L Jeannotte J Charron, Curr Biol 1999 9 7 369 372 10.1016/S0960-9822(99)80164-X 10209122
57. Mek2 is dispensable for mouse growth and development. LF Belanger S Roy M Tremblay B Brott AM Steff W Mourad P Hugo R Erikson J Charron, Mol Cell Biol 2003 23 14 4778 4787 12832465 10.1128/MCB.23.14.4778-4787.2003
58. Effects of U0126 and fibroblast growth factor on gene expression profile in Ciona intestinalis embryos as revealed by microarray analysis. E Sakabe N Tanaka N Shimozono T Gojobori S Fujiwara, Dev Growth Differ 2006 48 6 391 400 10.1111/j.1440-169X.2006.00877.x 16872452
59. Hedgehog signaling patterns the outgrowth of unpaired skeletal appendages in zebrafish. Y Hadzhiev Z Lele S Schindler SW Wilson P Ahlberg U Strahle F Muller, BMC Dev Biol 2007 7 75 17597528 10.1186/1471-213X-7-75
60. Analysis of FGF function in normal and no tail zebrafish embryos reveals separate mechanisms for formation of the trunk and the tail. K Griffin R Patient N Holder, Development 1995 121 9 2983 2994 7555724
61. Mutations affecting development of the midline and general body shape during zebrafish embryogenesis. M Brand CP Heisenberg RM Warga F Pelegri RO Karlstrom D Beuchle A Picker YJ Jiang M Furutani-Seiki FJ van Eeden M Granato P Haffter M Hammerschmidt DA Kane RN Kelsh MC Mullins J Odenthal C Nusslein-Volhard, Development 1996 123 129 142 9007235
62. A genetic screen for mutations affecting embryogenesis in zebrafish. W Driever L Solnica-Krezel AF Schier SC Neuhauss J Malicki DL Stemple DY Stainier F Zwartkruis S Abdelilah Z Rangini J Belak C Boggs, Development 1996 123 37 46 9007227
63. The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio. P Haffter M Granato M Brand MC Mullins M Hammerschmidt DA Kane J Odenthal FJ van Eeden YJ Jiang CP Heisenberg RN Kelsh M Furutani-Seiki E Vogelsang D Beuchle U Schach C Fabian C Nusslein-Volhard, Development 1996 123 1 36 9007226
64. Mutations affecting the formation of the notochord in the zebrafish, Danio rerio. J Odenthal P Haffter E Vogelsang M Brand FJ van Eeden M Furutani-Seiki M Granato M Hammerschmidt CP Heisenberg YJ Jiang DA Kane RN Kelsh MC Mullins RM Warga ML Allende ES Weinberg C Nusslein-Volhard, Development 1996 123 103 115 9007233
65. Mutations affecting development of the notochord in zebrafish. DL Stemple L Solnica-Krezel F Zwartkruis SC Neuhauss AF Schier J Malicki DY Stainier S Abdelilah Z Rangini E Mountcastle-Shah W Driever, Development 1996 123 117 128 9007234
66. Getting into the Golgi. SI Bannykh N Nishimura WE Balch, Trends Cell Biol 1998 8 1 21 25 10.1016/S0962-8924(97)01184-7 9695803
67. Vesicular transport: the core machinery of COPI recruitment and budding. W Nickel B Brugger FT Wieland, J Cell Sci 2002 115 Pt 16 3235 3240 12140255
68. p38 MAPK regulates COPII recruitment. L Wang JM Lucocq, Biochemical and Biophysical Research Communications 2007 363 2 317 321 10.1016/j.bbrc.2007.08. 175 17888403
69. Chemical genetics suggests a critical role for lysyl oxidase in zebrafish notochord morphogenesis. C Anderson SJ Bartlett JM Gansner D Wilson L He JD Gitlin RN Kelsh J Dowden, Mol Biosyst 2007 3 1 51 59 17216056 10.1039/b613673g
70. A delicate balance: homeostatic control of copper uptake and distribution. MM Pena J Lee DJ Thiele, J Nutr 1999 129 7 1251 1260 10395584
71. Menkes disease and Wilson disease: two sides of the same copper coin. Part I: Menkes disease. JH Menkes, Eur J Paediatr Neurol 1999 3 4 147 158 10.1016/S1090-3798(99)90048-X 10476363
72. Structural and functional diversity of lysyl oxidase and the LOX-like proteins. J Molnar KS Fong QP He K Hayashi Y Kim SF Fong B Fogelgren KM Szauter M Mink K Csiszar, Biochim Biophys Acta 2003 1647 1-2 220 224 12686136
73. Essential role of lysyl oxidases in notochord development. JM Gansner BA Mendelsohn KA Hultman SL Johnson JD Gitlin, Developmental Biology 2007 307 2 202 213 10.1016/j.ydbio.2007.04.029 17543297
74. Regulation of lysyl oxidase by basic fibroblast growth factor in osteoblastic MC3T3-E1 cells. EJ Feres-Filho GB Menassa PC Trackman, J Biol Chem 1996 271 11 6411 6416 10.1074/jbc.271.11.6411 8626440
75. Epigenetic inhibition of lysyl oxidase transcription after transformation by ras oncogene. S Contente K Kenyon P Sriraman S Subramanyan RM Friedman, Mol Cell Biochem 1999 194 1-2 79 91 10.1023/A:1006913122261 10391127
76. Lysyl oxidase regulates actin filament formation through the p130(Cas)/Crk/DOCK180 signaling complex. SL Payne MJ Hendrix DA Kirschmann, J Cell Biochem 2006 98 4 827 837 10.1002/jcb.20792 16440329
77. The zebrafish book. M Westerfield, University of Oregon Press 1993
78. Stages of embryonic development of the zebrafish. CB Kimmel WW Ballard SR Kimmel B Ullmann TF Schilling, Dev Dyn 1995 203 3 253 310 8589427
79. hsp47 and hsp70 gene expression is differentially regulated in a stress- and tissue-specific manner in zebrafish embryos. Z Lele S Engel PH Krone, Dev Genet 1997 21 2 123 133 10.1002/(SICI)1520-6408(1997)21:2<123::AID-DVG2>3. 0.CO;2-9 9332971
80. parachute/n-cadherin is required for morphogenesis and maintained integrity of the zebrafish neural tube. Z Lele A Folchert M Concha GJ Rauch R Geisler F Rosa SW Wilson M Hammerschmidt L Bally-Cuif, Development 2002 129 14 3281 3294 12091300