Human skin endothelial cells can express all 10 TLR genes and respond to respective ligands

Clinical and Vaccine Immunology

Volumn 15, Issue 1, 2008, Pages 138-146

  Fitzner, Nicole ^a   Clauberg, Sigrid ^a   Essmann, Frank ^a   Liebmann, Joerg ^a   Kolb Bachofen, Victoria ^a,b  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b Forschungsgruppe Immunbiologie   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ENDOTOXIN; INDUCIBLE NITRIC OXIDE SYNTHASE; INTERLEUKIN 8; LIPOPOLYSACCHARIDE; MESSENGER RNA; MYELOID DIFFERENTIATION FACTOR 88; TOLL LIKE RECEPTOR; TOLL LIKE RECEPTOR 1; TOLL LIKE RECEPTOR 10; TOLL LIKE RECEPTOR 2; TOLL LIKE RECEPTOR 3; TOLL LIKE RECEPTOR 4; TOLL LIKE RECEPTOR 5; TOLL LIKE RECEPTOR 6; TOLL LIKE RECEPTOR 7; TOLL LIKE RECEPTOR 8; TOLL LIKE RECEPTOR 9; LIGAND; MYD88 PROTEIN, HUMAN;

ARTICLE; CELL ACTIVATION; CELL COMPARTMENTALIZATION; CELL FUNCTION; CELL IMMORTALIZATION; CONTROLLED STUDY; CYTOKINE RELEASE; ENDOTHELIUM CELL; GENE EXPRESSION; GENE SEQUENCE; HUMAN; HUMAN CELL; INNATE IMMUNITY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; REAL TIME POLYMERASE CHAIN REACTION; SKIN CELL; BIOSYNTHESIS; BLOOD; CELL LINE; CHILD; CYTOLOGY; FUNCTIONS OF THE SKIN AND ITS APPENDAGES; GENETICS; IMMUNOLOGY; MALE; METABOLISM; PHYSIOLOGY; PRESCHOOL CHILD; SIGNAL TRANSDUCTION; SKIN;

CELL LINE; CHILD; CHILD, PRESCHOOL; ENDOTHELIAL CELLS; GENE EXPRESSION; HUMANS; IMMUNITY, NATURAL; INTERLEUKIN-8; LIGANDS; MALE; MYELOID DIFFERENTIATION FACTOR 88; NITRIC OXIDE SYNTHASE TYPE II; SIGNAL TRANSDUCTION; SKIN; SKIN PHYSIOLOGY; TOLL-LIKE RECEPTORS;

EID: 43149123808     PISSN: 15566811     EISSN: 1556679X     Source Type: Journal    
DOI: 10.1128/CVI.00257-07     Document Type: Article

References (27)

1. Bartz, H., Y. Mendoza, M. Gebker, T. Fischborn, K. Heeg, and A. Dalpke. 2004. Poly-guanosine strings improve cellular uptake and stimulatory activity of phosphodiester CpG oligonucleotides in human leukocytes. Vaccine 23:148-155.
2. Bourke, E., D. Bosisio, J. Golay, N. Polentarutti, and A. Mantovani. 2003. The toll-like receptor repertoire of human B lymphocytes: inducible and selective expression of TLR9 and TLR10 in normal and transformed cells. Blood 102:956-963.
3. Christopherson, K. W., Jr., and R. A. Hromas. 2004. Endothelial chemokines in autoimmune disease. Curr. Pharm. Des. 10:145-154.
4. Cristofaro, P., and S. M. Opal. 2003. The Toll-like receptors and their role in septic shock. Expert Opin. Ther. Targets 7:603-612.
5. Edfeldt, K., J. Swedenborg, G. K. Hansson, and Z. Q. Yan. 2002. Expression of toll-like receptors in human atherosclerotic lesions: a possible pathway for plaque activation. Circulation 105:1158-1161.
6. Equils, O., A. Shapiro, Z. Madak, C. Liu, and D. Lu. 2004. Human immunodeficiency virus type 1 protease inhibitors block Toll-like receptor 2 (TLR2)- and TLR4-induced NF-κB activation. Antimicrob. Agents Chemother. 48:3905-3911.
7. Faure, E., O. Equils, P. A. Sieling, L. Thomas, F. X. Zhang, C. J. Kirschning, N. Polentarutti, M. Muzio, and M. Arditi. 2000. Bacterial lipopolysaccharide activates NF-κB through Toll-like receptor 4 (TLR-4) in cultured human dermal endothelial cells. Differential expression of TLR-4 and TLR-2 in endothelial cells. J. Biol. Chem. 275:11058-11063.
8. Faure, E., L. Thomas, H. Xu, A. Medvedev, O. Equils, and M. Arditi. 2001. Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-κB activation. J. Immunol. 166:2018-2024.
9. Fitzner, N. 2007. Ph.D. thesis. University of Duesseldorf, Germany.
10. Fitzner, N., and V. Kolb-Bachofen. 2006. Functional expression of toll-like receptors (TLR's) on human endothelial cells activate the inducible nitric oxide synthase. Eur. J. Cell Biol. 85:114-115.
11. Gerhard-Wingender, N. G., B. Schumak, F. Juengerkes, E. Endl, D. von Bubnoff, J. Steitz, J. Striegler, G. Moldenhauer, T. Tüting, A. Heit, K. M. Huster, O. Takikawa, S. Akira, D. H. Busch, H. Wagner, G. J. Haemmerling, P. A. Knolle, and A. Limmer. 2006. Systemic application of CpG-rich DNA suppresses adaptive T cell immunity via induction of IDO. Eur. J. Immunol. 36:12-20.
12. Gillitzer, R., and M. Goebeler. 2001. Chemokines in cutaneous wound healing. J. Leukoc. Biol. 69:513-521.
13. Iacobas, D. A., S. Iacobas, W. E. I. Li, G. Zoidl, R. Dermietzel, and D. C. Spray. 2005. Genes controlling multiple functional pathways are transcriptionally regulated in connexin43 null mouse heart. Physiol. Genomics 20:211-223.
14. Kluger, M. S.2004. Vascular endothelial cell adhesion and signaling during leukocyte recruitment. Adv. Dermatol. 20:163-201.
15. Knolle, P. A., and A. Limmer. 2003. Control of immune responses by savenger liver endothelial cells. Swiss Med. Wkly. 133:501-506.
16. Köllisch, G., B. N. Kalali, V. Voelcker, R. Wallich, H. Behrendt, J. Ring, S. Bauer, T. Jakob, M. Mempel, and M. Ollert. 2005. Various members of the Toll-like receptor family contribute to the innate immune response of human epidermal keratinocytes. Immunology 114:531-541.
17. Kufer, T. A., D. J. Banks, and D. J. Philpott. 2006. Innate immune sensing of microbes by Nod proteins. Ann. N. Y. Acad. Sci. 1072:19-27.
18. Li, J., Z. Ma, Z. L. Tang, T. Stevens, B. Pitt, and S. Li. 2004. CpG DNA-mediated immune response in pulmonary endothelial cells. Am. J. Physiol. Lung Cell Mol. Physiol. 287:L552-L558.
19. Ozato, K., H. Tsujimura, and T. Tamura. 2002. Toll-like receptor signaling and regulation of cytokine gene expression in the immune system. BioTechniques 2002 Oct.(Suppl.):66-72.
20. Schwentker, A., and T. R. Billiar. 2003. Nitric oxide and wound repair. Surg. Clin. N. Am. 83:521-530.
21. Stallmeyer, B., H. Kampfer, N. Kolb, J. Pfeilschifter, and S. Frank. 1999. The function of nitric oxide in wound repair: inhibition of inducible nitric oxide-synthase severely impairs wound reepithelialization. J. Investig. Dermatol. 113:1090-1098.
22. Strober, W., P. J. Murray, A. Kitani, and T. Watanabe. 2006. Signalling pathways and molecular interactions of NOD1 and NOD2. Nat. Rev. Immunol. 6:9-20.
23. Takeda, K., and S. Akira. 2005. Toll-like receptors in innate immunity. Int. Immunol. 17:1-14.
24. Tobias, P., and L. K. Curtiss. 2005. Thematic review series: the immune system and atherogenesis. Paying the price for pathogen protection: toll receptors in atherogenesis. J. Lipid Res. 46:404-411.
25. Weller, R.2003. Nitric oxide: a key mediator in cutaneous physiology. Clin. Exp. Dermatol. 28:511-514.
26. Yamasaki, K., H. D. J. Edington, C. McClosky, E. Tzeng, A. Lizonova, I. Kovesdi, D. L. Steed, and T. R. Billiar. 1998. Reversal of impaired wound repair in iNOS-deficient mice by topical adenoviral-mediated iNOS gene transfer. J. Clin. Investig. 101:967-971.
27. Zarember, K. A., and P. J. Godowski. 2002. Tissue expression of human Toll-like receptors and differential regulation of Toll-like receptor mRNAs in leukocytes in response to microbes, their products, and cytokines. J. Immunol. 168:554-561.