Multiple pathways for triacylglycerol biosynthesis in Streptomyces coelicolor

Applied and Environmental Microbiology

Volumn 74, Issue 9, 2008, Pages 2573-2582

  Arabolaza, Ana ^a   Rodriguez, Eduardo ^a   Altabe, Silvia ^a   Alvarez, Hector ^b   Gramajo, Hugo ^a  

^a UNIVERSIDAD NACIONAL DE ROSARIO   (Argentina)

^b UNIVERSIDAD NACIONAL DE LA PATAGONIA SAN JUAN BOSCO   (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; BIOSYNTHESIS; ENZYMES; FATTY ACIDS; GENES; REACTION KINETICS;

MUTANT STRAINS; STREPTOMYCES COELICOLOR;

GLYCEROL;

ACYL COENZYME A; DIACYLGLYCEROL; DIACYLGLYCEROL ACYLTRANSFERASE 1; FATTY ACID; PHOSPHOLIPID; TRIACYLGLYCEROL;

FATTY ACID; GENE; LIPID; METABOLISM; SOIL MICROORGANISM;

ACINETOBACTER; ACINETOBACTER BAYLI; ARTICLE; BACTERIAL GENE; BACTERIAL METABOLISM; BACTERIUM MUTANT; CONTROLLED STUDY; ENZYME ACTIVITY; ESTERIFICATION; GENE DELETION; LIPID COMPOSITION; LIPID STORAGE; LIPOGENESIS; NONHUMAN; SCO0123 GENE; SCO0958 GENE; SCO1280 GENE; STREPTOMYCES COELICOLOR;

ACINETOBACTER; ACYL COENZYME A; ACYLTRANSFERASES; BACTERIAL PROTEINS; CLONING, MOLECULAR; GENE DELETION; GENE DOSAGE; GENE EXPRESSION; KINETICS; METABOLIC NETWORKS AND PATHWAYS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; STREPTOMYCES COELICOLOR; TRIGLYCERIDES;

ACINETOBACTER BAYLYI; STREPTOMYCES COELICOLOR;

EID: 43049147597     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02638-07     Document Type: Article

References (40)

1. Alvarez, H. M., R. Kaischeuer, and A. Steinbüchel. 2000. Accumulation and mobilization of storage lipids by Rhodococcus opacus PD630 and Rhodococcus ruber NCIMB 40126. Appl. Microbiol. Biotechnol. 54:218-223.
2. Alvarez, H. M., F. Mayer, D. Fabritius, and A. Steinbüchel. 1996. Formation of intracytoplasmic lipid inclusions by Rhodococcus opacus strain PD630. Arch. Microbiol. 165:377-386.
3. Alvarez, H. M., and A. Steinbüchel. 2002. Triacylglycerols in prokaryotic microorganisms. Appl. Microbiol. Biotechnol. 60:367-376.
4. Barksdale, L., and S. K. Kim. 1977. Mycobacterium. Bacteriol. Rev. 41:217-372.
5. Bouvier-Nave, P., P. Benveniste, P. Oelkers, S. L. Sturley, and H. Schaller. 2000. Expression in yeast and tobacco of plant cDNAs encoding acyl CoA: diacylglyccrol acyltransferase. Eur. J. Biochem. 267:85-96.
6. Bystrykh, L. V., M. A. Fernandez-Moreno, J. K. Herrema, F. Malpartida, D. A. Hopwood, and L. Dijkhuizen. 1996. Production of actinorhodin-related "blue pigments" by Streptomyces coelicolor A3(2). J. Bacteriol. 178:2238-2244.
7. Cases, S., S. J. Smith, Y. W. Zheng, H. M. Myers, S. R. Lear, E. Sande, S. Novak, C. Collins, C. B. Welch, A. J. Lusis, S. K. Erickson, and R. V. Farese, Jr. 1998. Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proc. Natl. Acad. Sci. USA 95:13018-13023.
8. Cases, S., S. J. Stone, P. Zhou, E. Yen, B. Tow, K. D. Lardizabal, T. Voelker, and R. V. Farese, Jr. 2001. Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members. J. Biol. Chem. 276:38870-38876.
9. Dahlqvist, A., U. Stahl, M. Lenman, A. Banas, M. Lee, L. Sandager, H. Ronne, and S. Stymne. 2000. Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants. Proc. Natl. Acad. Sci. USA 97:6487-6492.
10. Daniel, J., C. Deb, V. S. Dubey, T. D. Sirakova, B. Abomoelak, H. R. Morbidoni, and P. E. Kolattukudy. 2004. Induction of a novel class of diacylglycerol acyltransferases and triacylglycerol accumulation in Mycobacterium tuberculosis as it goes into a dormancy-like state in culture. J. Bacteriol. 186:5017-5030.
11. Glomset, J. A. 1968. The plasma lecithinsxholesterol acyltransferase reaction. J. Lipid Res. 9:155-167.
12. Gregory, M. A., R. Till, and M. C. M. Smith. 2003. Integration site for Streptomyces phage φBT1 and development of site-specific integrating vectors. J. Bacteriol. 185:5320-5323.
13. Guzman, L. M., D. Belin, M. J. Carson, and J. Beckwith. 1995. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177:4121-4130.
14. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
15. Herron, P. R., G. Hughes, G. Chandra, S. Fielding, and P. J. Dyson. 2004. Transposon Express, a software application to report the identity of insertions obtained by comprehensive transposon mutagenesis of sequenced genomes: analysis of the preference for in vitro Tn5 transposition into GC-rich DNA. Nucleic Acids Res. 32:e113.
16. Hobbs, D. H., C. Lu, and M. J. Hills. 1999. Cloning of a cDNA encoding diacylglycerol acyltransferase from Arabidopsis thaliana and its functional expression. FEBS Lett. 452:145-149.
17. Hoischen, C., K. Gura, C. Luge, and J. Gumpert. 1997. Lipid and fatty acid composition of cytoplasmic membranes from Streptomyces hygroscopicus and its stable protoplast-type L form. J. Bacteriol. 179:3430-3436.
18. Kalscheuer, R., and A. Steinbüchel. 2003. A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1. J. Biol. Chem. 278:8075-8082.
19. Kalscheuer, R., T. Stoveken, U. Malkus, R. Reichelt, P. N. Golyshin, J. S. Sabirova, M. Ferrer, K. N. Timmis, and A. Steinbüchel. 2007. Analysis of storage lipid accumulation in Alcanivorax borkumensis: evidence for alternative triacylglycerol biosynthesis routes in bacteria. J. Bacteriol. 189:918-928.
20. Kieser, T., M. J. Bibb, M. J. Buttner, K. F. Chater, and D. A. Hopwood. 2000. Practical Streptomyces genetics. John Innes Centre, Norwich, United Kingdom.
21. Lehner, R., and A. Kuksis. 1993. Triacylglycerol synthesis by an sn-1,2(2,3)-diacylglycerol transacylase from rat intestinal microsomes. J. Biol. Chem. 268:8781-8786.
22. Lehner, R., and A. Kuksis. 1996. Biosynthesis of triacylglycerols. Prog. Lipid Res. 35:169-201.
23. Makula, R. A., P. J. Lockwood, and W. R. Finnerty. 1975. Comparative analysis of the lipids of Acinetobacter species grown on hexadecane. J. Bacteriol. 121:250-258.
24. Miroux, B., and J. E. Walker. 1996. Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J. Mol. Biol. 260:289-298.
25. Oelkers, P., A. Tinkelenberg, N. Erdeniz, D. Cromley, J. T. Billheimer, and S. L. Sturley. 2000. A lecithin cholesterol acyltransferase-like gene mediates diacylglycerol esterification in yeast. J. Biol. Chem. 275:15609-15612.
26. Olukoshi, E. R., and N. M. Packter. 1992. Isolation of triacylglycerol syn thase activity from Streptomyces lividans. Biochem. Soc. Trans. 20:99S.
27. Olukoshi, E. R., and N. M. Packter. 1994. Importance of stored triacylglycerols in Streptomyces: possible carbon source for antibiotics. Microbiology 140:931-943.
28. E, a sigma factor required for normal cell wall integrity in Streptomyces coelicolor A3(2). Mol. Microbiol. 33:97-107.
29. Plaskitt, K. A., and K. F. Chater. 1995. Influence of developmental genes on localized glycogen deposition in colonies of a mycelial prokaryote. Streptomyces coelicolor A3(2): possible interface between metabolism and morphogenesis. Philos. Trans. R. Soc. 347:105-121.
30. Raetz, C. R., and K. F. Newman. 1978. Neutral lipid accumulation in the membranes of Escherichia coli mutants lacking diglyceride kinase. J. Biol. Chem. 253:3882-3887.
31. Routaboul, J. M., C. Benning, N. Bechtold, M. Caboche, and L. Lepiniec. 1999. The TAG1 locus of Arabidopsis encodes for a diacylglycerol acyltransferase. Plant Physiol. Biochem. 37:831-840.
32. Sandager, L., M. H. Gustavsson, U. Stahl, A. Dahlqvist, E. Wiberg, A. Banas, M. Lenman, H. Ronne, and S. Stymne. 2002. Storage lipid synthesis is non-essential in yeast. J. Biol. Chem. 277:6478-6482.
33. Sirakova, T. D., V. S. Dubey, C. Deb, J. Daniel, T. A. Korotkova, B. Abomoelak, and P. E. Kolattukudy. 2006. Identification of a diacylglycerol acyltransferase gene involved in accumulation of triacylglycerol in Mycobacterium tuberculosis under stress. Microbiology 152:2717-2725.
34. Sorger, D., and G. Daum. 2003. Triacylglycerol biosynthesis in yeast. Appl. Microbiol. Biotechnol. 61:289-299.
35. Stahl, U., A. S. Carlsson, M. Lenman, A. Dahlqvist, B. Huang, W. Banas, A. Banas, and S. Stymne. 2004. Cloning and functional characterization of a phospholipid:diacylglycerol acyltransferase from Arabidopsis. Plant Physiol. 135:1324-1335.
36. Stobart, K., M. Mancha, M. Lenman, A. Dahlqvist, and S. Stymne. 1997. Triacylglycerols are synthesised and utilized by transacylation reactions in microsomal preparations of developing safflower (Carthamus tinctorius L.) seeds. Planta 203:58-66.
37. Stoveken, T., R. Kalscheuer, U. Malkus, R. Reichelt, and A. Steinbüchel. 2005. The wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1: characterization of a novel type of acyltransferase. J. Bacteriol. 187:1369-1376.
38. Studier, F. W., and B. A. Moffatt. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:113-130.
39. Waltermann, M., H. Luftmann, D. Baumeister, R. Kalscheuer, and A. Steinbüchel. 2000. Rhodococcus opacus strain PD630 as a new source of high-value single-cell oil? Isolation and characterization of triacylglycerols and other storage lipids. Microbiology 146:1143-1149.
40. Waltermann, M., T. Stoveken, and A. Steinbüchel. 2007. Key enzymes for biosynthesis of neutral lipid storage compounds in prokaryotes: properties, function and occurrence of wax ester synthases/acyl-CoA: diacylglycerol acyltransferases. Biochimie 89:230-242.