Repeated enzyme immunoassays have limited utility in diagnosing Clostridium difficile

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 27, Issue 5, 2008, Pages 397-399

  Drees, M ^a,b,c   Snydman, D R ^a,b   O'Sullivan, C E ^a,b,d  

^a TUFTS MEDICAL CENTER   (United States)

^b TUFTS UNIVERSITY   (United States)

^c CHRISTIANA CARE HEALTH SYSTEM   (United States)

^d BARTS HEALTH NHS TRUST   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ARTICLE; BACTERIAL INFECTION; CALCULATION; CLOSTRIDIUM DIFFICILE; COHORT ANALYSIS; CONTROLLED STUDY; DIAGNOSTIC VALUE; ENZYME IMMUNOASSAY; FEMALE; HUMAN; LABORATORY DIAGNOSIS; MAJOR CLINICAL STUDY; MALE; PREDICTION AND FORECASTING; PRIORITY JOURNAL; REPEAT PROCEDURE; RETROSPECTIVE STUDY;

CLOSTRIDIUM DIFFICILE; COHORT STUDIES; ENTEROCOLITIS, PSEUDOMEMBRANOUS; FEMALE; HUMANS; IMMUNOENZYME TECHNIQUES; MALE; MIDDLE AGED; PREDICTIVE VALUE OF TESTS; RETROSPECTIVE STUDIES;

EID: 43049123374     PISSN: 09349723     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10096-007-0452-8     Document Type: Article

References (13)

1. Loo VG, Poirier L, Miller MA, Oughton M, Libman MD, Michaud S, Bourgault AM, Nguyen T, Frenette C, Kelly M, Vibien A, Brassard P, Fenn S, Dewar K, Hudson TJ, Horn R, Rene P, Monczak Y, Dascal A (2005) A predominantly clonal multi-institutional outbreak of Clostridium difficile-associated diarrhea with high morbidity and mortality. N Engl J Med 353:2442-2449
2. McDonald LC, Owings M, Jernigan DB (2006) Clostridium difficile infection in patients discharged from US short-stay hospitals, 1996-2003. Emerging Infect Dis 12:409-415
3. O'Connor D, Hynes P, Cormican M, Collins E, Corbett-Feeney G, Cassidy M (2001) Evaluation of methods for detection of toxins in specimens of feces submitted for diagnosis of Clostridium difficile-associated diarrhea. J Clin Microbiol 39:2846-2849
4. Gerding DN, Johnson S, Peterson LR, Mulligan ME, Silva J Jr (1995) Clostridium difficile-associated diarrhea and colitis. Infect Control Hosp Epidemiol 16:459-477
5. Aldeen WE, Bingham M, Aiderzada A, Kucera J, Jense S, Carroll KC (2000) Comparison of the TOX A/B test to a cell culture cytotoxicity assay for the detection of Clostridium difficile in stools. Diagn Microbiol Infect Dis 36:211-213
6. Mohan SS, McDermott BP, Parchuri S, Cunha BA (2006) Lack of value of repeat stool testing for Clostridium difficile toxin. Am J Med 119:356-358
7. Manabe YC, Vinetz JM, Moore RD, Merz C, Charache P, Bartlett JG (1995) Clostridium difficile colitis: an efficient clinical approach to diagnosis. Ann Intern Med 123:835-840
8. van den Berg RJ, Vaessen N, Endtz HP, Schulin T, van d V, Kuijper EJ (2007) Evaluation of real-time PCR and conventional diagnostic methods for the detection of Clostridium difficile-associated diarrhoea in a prospective multicentre study. J Med Microbiol 56:36-42
9. Gleaves C, Aldeen R, Schwartz K, CampbellM, Carroll K (2000) Evaluation of the Premier Toxins A & B EIA for the Detection of C. difficile from clinical stool specimens. In: Program and abstracts of the 100th American Society of Microbiology General Meeting. Abstract C-252
10. Renshaw AA, Stelling JM, Doolittle MH (1996) The lack of value of repeated Clostridium difficile cytotoxicity assays. Arch Pathol Lab Med 120:49-52
11. Borek AP, Aird DZ, Carroll KC (2005) Frequency of sample submission for optimal utilization of the cell culture cytotoxicity assay for detection of Clostridium difficile toxin. J Clin Microbiol 43:2994-2995
12. Taylor Z, Nolan CM, Blumberg HM (2005) Controlling tuberculosis in the United States. Recommendations from the American Thoracic Society, CDC, and the Infectious Diseases Society of America. MMWR Recomm Rep 54:1-81
13. Ticehurst JR, Aird DZ, Dam LM, Borek AP, Hargrove JT, Carroll KC (2006) Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin. J Clin Microbiol 44:1145-1149