The lack of value of repeated Clostridium difficile cytotoxicity assays

Archives of Pathology and Laboratory Medicine

Volumn 120, Issue 1, 1996, Pages 49-52

  Renshaw, Andrew A ^a   Stelling, John M ^a   Doolittle, Marc H ^a,b  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

^b NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CLINICAL ARTICLE; CLINICAL FEATURE; CLOSTRIDIUM DIFFICILE; CYTOTOXICITY; DIARRHEA; HUMAN; LABORATORY DIAGNOSIS; PSEUDOMEMBRANOUS COLITIS;

EID: 0030050163     PISSN: 00039985     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Kelly CP, Pothoulakis C, LaMont JT. Clostridium difficile colitis. N Engl J Med. 1994;330:257-262.
2. Siegel DL, Edelstein PH. Inappropriate testing for diarrheal diseases in the hospital. JAMA. 1990;263:979-982.
3. Yannelli B, Gurevich I, Schoch PE, Cunha BA. Yield of stool cultures, ova and parasite tests, and Clostridium difficile determination in nosocomial diarrheas. Am J Infect Control. 1988;16:246-249.
4. Fan K, Morris AJ, Reller LB. Application of rejection criteria for stool cultures lor bacterial enteric pathogens. J Clin Microbiol. 1993;31:2233-2235.
5. Larson HF, Parry JV, Price AB, Davies DR, Dolby I, Tyrrell DAJ. Undescribed toxin in pseudomembranous colitis. Br Med J. 1977;1:1246-1248.
6. Bartlett J, Chang TW, Gurwith M, et al. Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia. N Engl J Med. 1978;298:531-534.
7. DeGirolami PC, Hanff PA, Eichelberger K, et al. Multicenter evaluation of a new enzyme immunoassay for detection of Clostridium difficile enterotoxin A. J Clin Microbiol. 1992;30:1085-1088.
8. Doern GV, Coughlin RT, Wu L. Laboratory diagnosis of Clostridium difficile-associated gastrointestinal disease: comparison of a monoclonal antibody enzyme immunoassay for toxins A and B with a monoclonal antibody enzyme immunoassay for toxin A only and two cytotoxicity assays. J Clin Microbiol. 1992;30: 2042-2046.
9. DiPersio JR, Varga FJ, Conwell DL, et al. Development of a rapid enzyme immunoassay for Clostridium difficile toxin A and its use in the diagnosis of C. difficile-associated disease. J Clin Microbiol. 1991;29:2724-2730.
10. Barbut F, Kajzer C, Planas N, Petit J. Comparison of three enzyme immunoassays, a cytotoxicity assay, and toxigenic culture for diagnosis of Clostridium difficile-associated diarrhea. J Clin Microbiol. 1993;31:963-967.
11. Lyerly D, Krivan H, Wilkins T. Clostridium difficile: its disease and toxins. Clin Microbiol Rev. 1988;1:1-18.
12. Chang TW, Gorbach S, Bartlet J. Neutralization of Clostridium difficile toxin by Clostridium sordellii antitoxins. Infect Immun. 1978;22:418-22.
13. Keighley MRB, Burdon DW, Arabi Y, et al. Randomised controlled trial of vancomycin for pseudomembranous colitis and postoperative diarrhea. Br Med J. 1978;2:1667-1669.
14. Tedesco F, Markham R, Gurwith M, et al. Oral vancomycin for antibiotic-associated pseuriomembranous colitis. Lancet. 1978;2:226-228.
15. Larson HF, Price AB, Honour P. Clostridium difficile and the etiology of pseudomembranous colitis. Lancet. 1978;1:1063-1066.
16. Miles BL, Siders JA, Allen SD. Evaluation of a commercial latex test for Clostridium difficile for reactivity with C. difficile and cross-reactions with other bacteria. J Clin Microbiol. 1988;26:2452-2455.
17. Lyerly DM, Barroso LA, Wilkins TD. Identification of the latex test-reactive protein of Clostridium difficile as glutamate dehydrogenase. J Clin Microbiol. 1991;29:2639-2642.
18. Woods GL, Iwen PC. Comparison of a dot immunobinding assay, latex agglutination, and cytotoxin assay for laboratory diagnosis of Clostridium difficile - associated diarrhea. J Clin Microbiol. 1990;28:855-857.