On-gel fluorescent visualization and the site identification of S-nitrosylated proteins

Analytical Biochemistry

Volumn 377, Issue 2, 2008, Pages 150-155

  Han, Peiwei ^a,b   Zhou, Xixi ^a,b   Huang, Bo ^a,b   Zhang, Xu ^a,b   Chen, Chang ^a  

^a INSTITUTE OF BIOPHYSICS   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

AMCA switch; Biotin switch; Fluorescence; LC MS MS; Nitric oxide; S nitrosylation; Site identification

Indexed keywords

AMINO ACIDS; CELL SIGNALING; COENZYMES; FLUOROPHORES; LIQUID CHROMATOGRAPHY; MAMMALS; MASS SPECTROMETRY; NITRIC OXIDE; VISUALIZATION;

BOVINE SERUM ALBUMINS; DIRECT VISUALIZATION; LC-MS/MS; LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC MS/MS); POTENTIAL INTERFERENCES; S-NITROSYLATION; SITE IDENTIFICATION; ULTRA-VIOLET LIGHT;

FLUORESCENCE;

7 AMINO 4 METHYLCOUMARIN 3 ACETIC ACID; BRILLIANT BLUE; CYSTEINE; NITROSOCYSTEINE; S NITROSYLATED PROTEIN;

ARTICLE; FLUORESCENCE ANALYSIS; IMMUNOCHEMISTRY; LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; TANDEM MASS SPECTROMETRY; ULTRAVIOLET RADIATION;

BOVINAE;

EID: 43049115279     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.03.023     Document Type: Article

References (29)

1. C.M. Padgett A.R. Whorton S -Nitrosoglutathione reversibly inhibits GAPDH by S-nitrosylation Am. J. Physiol. 269 1995 C739 C749
2. M.R. Hara N. Agrawal S.F. Kim M.B. Cascio M. Fujimuro Y. Ozeki M. Takahashi J.H. Cheah S.K. Tankou L.D. Hester C.D. Ferris S.D. Hayward S.H. Snyder A. Sawa S-nitrosylated GAPDH initiates apoptotic cell death by nuclear translocation following Siah1 binding Nat. Cell Biol. 7 2005 665 674
3. M.R. Hara S.H. Snyder Nitric oxide–GAPDH–Siah: A novel cell death cascade Cell Mol. Neurobiol. 26 2006 527 538
4. L. Xu J.P. Eu G. Meissner J.S. Stamler Activation of the cardiac calcium release channel (ryanodine receptor) by poly-S-nitrosylation Science 279 1998 234 237
5. J. Sun C. Xin J.P. Eu J.S. Stamler G. Meissner Cysteine-3635 is responsible for skeletal muscle ryanodine receptor modulation by NO Proc. Natl. Acad. Sci. USA 98 2001 11158 11162
6. H.E. Marshall J.S. Stamler Inhibition of NF–kappa B by S-nitrosylation Biochemistry 40 2001 1688 1693
7. H.E. Marshall D.T. Hess J.S. Stamler S-nitrosylation: Physiological regulation of NF–kappa B Proc. Natl. Acad. Sci. USA 101 2004 8841 8842
8. S. Mohr B. Zech E.G. Lapetina B. Brune Inhibition of caspase-3 by S-nitrosation and oxidation caused by nitric oxide Biochem. Biophys. Res. Commun. 238 1997 387 391
9. T. Uehara T. Nakamura D. Yao Z.Q. Shi Z. Gu Y. Ma E. Masliah Y. Nomura S.A. Lipton S-nitrosylated protein–disulphide isomerase links protein misfolding to neurodegeneration Nature 441 2006 513 517
10. D. Yao Z. Gu T. Nakamura Z.Q. Shi Y. Ma B. Gaston L.A. Palmer E.M. Rockenstein Z. Zhang E. Masliah T. Uehara S.A. Lipton Nitrosative stress linked to sporadic Parkinson’s disease: S-nitrosylation of parkin regulates its E3 ubiquitin ligase activity Proc. Natl. Acad. Sci. USA 101 2004 10810 10814
11. I. Dalle-Donne A. Milzani D. Giustarini S.P. Di R. Colombo R. Rossi S-NO-actin: S-nitrosylation kinetics and the effect on isolated vascular smooth muscle J. Muscle Res. Cell Motil. 21 2000 171 181
12. S.R. Jaffrey H. Erdjument-Bromage C.D. Ferris P. Tempst S.H. Snyder Protein S-nitrosylation: A physiological signal for neuronal nitric oxide Nat. Cell Biol. 3 2001 193 197
13. J.S. Stamler O. Jaraki J. Osborne D.I. Simon J. Keaney J. Vita D. Singel C.R. Valeri J. Loscalzo Nitric oxide circulates in mammalian plasma primarily as an S-nitroso adduct of serum albumin Proc. Natl. Acad. Sci. USA 89 1992 7674 7677
14. K. Fang N.V. Ragsdale R.M. Carey T. MacDonald B. Gaston Reductive assays for S -nitrosothiols: Implications for measurements in biological systems Biochem. Biophys. Res. Commun. 252 1998 535 540
15. L. Zhukova I. Zhukov W. Bal A. Wyslouch-Cieszynska Redox modifications of the C-terminal cysteine residue cause structural changes in S100A1 and S100B proteins Biochim. Biophys. Acta 1742 2004 191 201
16. L.M. Mikesh B. Ueberheide A. Chi J.J. Coon J.E. Syka J. Shabanowitz D.F. Hunt The utility of ETD mass spectrometry in proteomic analysis Biochim. Biophys. Acta 1764 2006 1811 1822
17. T. Kuncewicz E.A. Sheta I.L. Goldknopf B.C. Kone Proteomic analysis of S-nitrosylated proteins in mesangial cells Mol. Cell Proteomics 2 2003 156 163
18. C. Lindermayr G. Saalbach J. Durner Proteomic identification of S-nitrosylated proteins in Arabidopsis Plant Physiol. 137 2005 921 930
19. G. Hao B. Derakhshan L. Shi F. Campagne S.S. Gross SNOSID, a proteomic method for identification of cysteine S-nitrosylation sites in complex protein mixtures Proc. Natl. Acad. Sci. USA 103 2006 1012 1017
20. T.M. Greco R. Hodara I. Parastatidis H.F. Heijnen M.K. Dennehy D.C. Liebler H. Ischiropoulos Identification of S-nitrosylation motifs by site-specific mapping of the S -nitrosocysteine proteome in human vascular smooth muscle cells Proc. Natl. Acad. Sci. USA 103 2006 7420 7425
21. S. Camerini M.L. Polci U. Restuccia V. Usuelli A. Malgaroli A. Bachi A novel approach to identify proteins modified by nitric oxide: The His-tag switch method J. Proteome Res. 6 2007 3224 3231
22. K. Sandau B. Brune The dual role of S -nitrosoglutathione (GSNO) during thymocyte apoptosis Cell. Signal. 8 1996 173 177
23. A. Shevchenko H. Tomas J. Havlis J.V. Olsen M. Mann In-gel digestion for mass spectrometric characterization of proteins and proteomes Nat. Protoc. 1 2006 2856 2860
24. K. Hirayama S. Akashi M. Furuya K. Fukuhara Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and Frit–FAB LC/MS Biochem. Biophys. Res. Commun. 173 1990 639 646
25. J.P. Fabisiak A. Sedlov V.E. Kagan Quantification of oxidative/nitrosative modification of Cys34 in human serum albumin using a fluorescence-based SDS–PAGE assay Antioxid. Redox Signal. 4 2002 855 865
26. J.S. Stamler E.J. Toone S.A. Lipton N.J. Sucher (S)NO signals: Translocation, regulation, and a consensus motif Neuron 18 1997 691 696
27. M. Hollinshead J. Sanderson D.J. Vaux Anti-biotin antibodies offer superior organelle-specific labeling of mitochondria over avidin or streptavidin J. Histochem. Cytochem. 45 1997 1053 1057
28. M. King O. Gildemeister B. Gaston J.B. Mannick Assessment of S -nitrosothiols on diaminofluorescein gels Anal. Biochem. 346 2005 69 76
29. K. Ckless N.L. Reynaert D.J. Taatjes K.M. Lounsbury A. van der Vliet Y. Janssen-Heininger In situ detection and visualization of S-nitrosylated proteins following chemical derivatization: Identification of Ran GTPase as a target for S-nitrosylation Nitric Oxide 11 2004 216 227