In vivo methods to assess polyadenylation efficiency

Methods in Molecular Biology

Volumn 419, Issue , 2008, Pages 171-185

  Hague, Lisa K ^a   Hall Pogar, Tyra ^a   Lutz, Carol S ^a  

^a NONE

Author keywords

3 UTR; Cis acting core and auxiliary elements; Luciferase assays; Polyadenylation; RNase protection assays; Transfection

Indexed keywords

LUCIFERASE; MESSENGER RNA; RIBONUCLEASE;

ARTICLE; BIOSYNTHESIS; GENE EXPRESSION; GENE VECTOR; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; GENETICS; HELA CELL; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; NUCLEIC ACID PROBE; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYADENYLATION;

ANTISENSE ELEMENTS (GENETICS); BASE SEQUENCE; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION; GENETIC VECTORS; HELA CELLS; HUMANS; LUCIFERASES; PLASMIDS; POLYADENYLATION; RIBONUCLEASES; RNA, MESSENGER; TRANSCRIPTION, GENETIC; TRANSFECTION;

EID: 42949128099     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-033-1_12     Document Type: Article

References (9)

1. Jacobson A, Peltz SW. 1996. Interrelationships of the pathways of mRNA decay and translation in eukaryotic cells. Annu Rev Biochem 65: 693-739.
2. Sachs AB, Sarnow P, Hentze MW. 1997. Starting at the beginning, middle and end: translation initiation in eukaryotes Cell 89: 831-838.
3. Wickens M, Anderson P, Jackson RJ. 1997. Life and death in the cytoplasm: messages from the 3' end. Curr Opin Genet Dev 7: 220-232.
4. Edmonds M. 2002. A history of polyA sequences: from formation to factors to function. Prog Nucleic Acid Res Mol Biol 71: 285-389.
5. Zhao J, Hyman L, Moore C. 1999. Formation of mRNA 3' ends in eukaryotes: mechanism, regulation and interrelationships with other steps in mRNA synthesis. Microbiol Mol Biol Rev 63: 405-445.
6. Hu J, Lutz CS, Wilusz J, Tian B. 2005. Bioinformatic identification of candidate cis-regulatory elements involved in human mRNA polyadenylation. RNA 11: 1485-1493.
7. Tian B, Hu J, Zhang H, Lutz CS. 2005. A large-scale analysis of mRNA polyadenylation of human and mouse genes. Nucleic Acids Res 33: 201-212.
8. Natalizio BJ, Muniz LC, Arhin GK, Wilusz J, Lutz CS. 2002. Upstream elements present in the 3' untranslated region of collagen genes influence the processing efficiency of overlapping polyadenylation signals. J Biol Chem 277: 42733-42740.
9. Hall-Pogar T, Zhang H, Tian B, and Lutz, CS. 2005. Alternative polyadenylation of cyclooxygenase-2. Nucleic Acids Res 33: 2565-2579.