Adaptation of the Dichlorofluorescein Assay for Detection of Radiation-Induced Oxidative Stress in Cultured Cells

Radiation Research

Volumn 160, Issue 6, 2003, Pages 622-630

  Wan, X Steven ^a   Zhou, Zhaozong ^a   Kennedy, Ann R ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETOACETIC ACID; DICHLOROFLUORESCEIN; FLUORESCEIN;

ADAPTATION; ARTICLE; ASSAY; CELL CULTURE; CONTROLLED STUDY; FLUORESCENCE; GAMMA RADIATION; HUMAN; HUMAN CELL; OXIDATIVE STRESS; PRIORITY JOURNAL; RADIATION DOSE;

BREAST NEOPLASMS; CELL LINE, TUMOR; FEMALE; FLUORESCEINS; FLUORESCENCE; HUMANS; OXIDATION-REDUCTION; OXIDATIVE STRESS;

EID: 0346123227     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.1667/3099     Document Type: Article

References (53)

1. H. Sies, Oxidative Stress. Academic Press, New York, 1985.
2. E. R. Stadtman and R. L. Levine, Protein oxidation. Ann. NY Acad. Sci. 899, 191-208 (2000).
3. S. Yla-Herttuala, Oxidized LDL and atherogenesis. Ann. NY Acad. Sci. 874, 134-137 (1999).
4. L. J. Marnett, Oxyradicals and DNA damage. Carcinogenesis 21, 361-370 (2000).
5. A. Giacosa and R. Filiberti, Free radicals, oxidative damage and degenerative diseases. Eur. J. Cancer Prev. 5, 307-312 (1996).
6. N. Hogg, Free radicals in disease. Semin. Reprod. Endocrinol. 16, 241-248 (1998).
7. J. S. Ross, N. E. Stagliano, M. J. Donovan, R. E. Breitbart and G. S. Ginsburg, Atherosclerosis: A cancer of the blood vessels? Am. J. Clin. Pathol. 116, S97-S107 (2001).
8. J. L. Witztum and D. Steinberg, The oxidative modification hypothesis of atherosclerosis: Does it hold for humans? Trends Cardiovasc. Med. 11, 93-102 (2001).
9. P. Kovacic and J. D. Jacintho, Mechanisms of carcinogenesis: Focus on oxidative stress and electron transfer. Curr. Med. Chem. 8, 773-796 (2001).
10. C. B. Ambrosone, Oxidants and antioxidants in breast cancer. Antioxid. Redox Signal 2, 903-917 (2000).
11. H. E. Poulsen, H. Prieme and S. Loft, Role of oxidative DNA damage in cancer initiation and promotion. Eur. J. Cancer Prev. 7, 9-16 (1998).
12. P. Jenner, Oxidative stress in Parkinson's disease and other neurodegenerative disorders. Pathol. Biol. 44, 57-64 (1996).
13. B. Drukarch and F. L. van Muiswinkel, Neuroprotection for Parkinson's disease: A new approach for a new millennium. Expert Opin. Investig. Drugs 10, 1855-1868 (2001).
14. G. Aliev, M. A. Smith, D. Seyidov, M. L. Neal, B. T. Lamb, A. Nunomura, E. K. Gasimov, H. V. Vinters and G. Perrylast, The role of oxidative stress in the pathophysiology of cerebrovascular lesions in Alzheimer's disease. Brain Pathol. 12, 21-35 (2002).
15. E. E. Tuppo and L. J. Forman, Free radical oxidative damage and Alzheimer's disease. J. Am. Osteopath. Assoc. 101, S11-15 (2001).
16. R. K. Schmidt-Ullrich, P. Dent, S. Grant, R. B. Mikkelsen and K. Valerie, Signal transduction and cellular radiation responses. Radiat. Res. 153, 245-257 (2000).
17. E. G. Wright, Radiation-induced genomic instability in haemopoietic cells. Int. J. Radiat. Biol. 74, 681-687 (1998).
18. D. R. Boreham and R. E. Mitchel, DNA lesions that signal the induction of radioresistance and DNA repair in yeast. Radiat. Res. 128, 19-28 (1991).
19. B. Halliwell, Mechanisms involved in the generation of free radicals. Pathol. Biol. 44, 6-13 (1996).
20. -), superoxide dismutases, and related matters. J. Biol. Chem. 272, 18515-18517 (1997).
21. B. Halliwell and J. M. C. Gutteridge, Free Radicals in Biology and Medicine, 3rd ed. Oxford University Press, Oxford, 1999.
22. Y. Miura and T. Ozawa, Noninvasive study of radiation-induced oxidative damage using in vivo electron spin resonance. Free Radic. Biol. Med. 28, 854-859 (2000).
23. S. J. Shin, K. Yamada, A. Sugisawa, K. Saito, T. Miyajima and K. Umegaki, Enhanced oxidative damage induced by total body irradiation in mice fed a low protein diet. Int. J. Radiat. Biol. 78, 425-432 (2002).
24. A. Agrawal, D. Chandra and R. K. Kale, Radiation induced oxidative stress: II studies in liver as a distant organ of tumor bearing mice. Mol. Cell. Biochem. 224, 9-17 (2001).
25. A. Valenzuela, The biological significance of malondialdehyde determination in the assessment of tissue oxidative stress. Life Sci. 48, 301-309 (1991).
26. A. W. Girotti, Lipid hydroperoxide generation, turnover, and effector action in biological systems. J. Lipid Res. 39, 1529-1542 (1998).
27. M. Chevion, E. Berenshtein and E. R. Stadtman, Human studies related to protein oxidation: Protein carbonyl content as a marker of damage. Free Radic. Res. 33, S99-108 (2000).
28. M. F. Beal, Oxidatively modified proteins in aging and disease. Free Radic. Biol. Med. 32, 797-803 (2002).
29. M. G. Simic, DNA markers of oxidative processes in vivo: Relevance to carcinogenesis and anticarcinogenesis. Cancer Res. 54, 1918s-1923s (1994).
30. H. Bartsch and J. Nair. Ultrasensitive and specific detection methods for exocyclic DNA adducts: Markers for lipid peroxidation and oxidative stress. Toxicology 153, 105-114 (2000).
31. D. A. Bass, J. W. Parce, L. R. Dechatelet, P. Szejda, M. C. Seeds and M. Thomas, Flow cytometric studies of oxidative product formation by neutrophils: A graded response to membrane stimulation. J. Immunol. 130, 1910-1917 (1983).
32. C. P. LeBel, H. Ischiropoulos and S. C. Bondy, Evaluation of the probe 2′,7′-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress. Chem. Res. Toxicol. 5, 227-231 (1992).
33. H. Ischiropoulos, A. Gow, S. R. Thom, N. W. Kooy, J. A. Royall and J. P. Crow, Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123. Methods Enzymol. 301. 367-373 (1999).
34. M. J. Burkitt and P. Wardman, Cytochrome C is a potent catalyst of dichlorofluorescin oxidation: implications for the role of reactive oxygen species in apoptosis. Biochem. Biophys. Res. Commun. 282, 329-233 (2001).
35. T. Ohashi, A. Mizutani, A. Murakami, S. Kojo, T. Ishii and S. Taketani, Rapid oxidation of dichlorodihydrofluorescin with heme and hemoproteins: Formation of the fluorescein is independent of the generation of reactive oxygen species. FEBS Lett. 511, 21-27 (2002).
36. P. Wardman, M. J. Burkitt, K. B. Patel, A. Lawrence, C. M. Jones, S. A. Everett and B. Vojnovic, Pitfalls in the use of common luminescent probes for oxidative and nitrosative stress. J. Fluorescence 12, 65-68 (2002).
37. T. G. Rossman and E. I. Goncharova, Spontaneous mutagenesis in mammalian cells is caused mainly by oxidative events and can be blocked by antioxidants and metallothionein. Mutat. Res. 402, 103-110 (1998).
38. D. W. Killilea, R. Hester, R. Balczon, P. Babal and M. N. Gillespie, Free radical production in hypoxic pulmonary artery smooth muscle cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 279, L408-L412 (2000).
39. G. M. Grabowski, J. D. Paulauskis and J. J. Godleski, Mediating phosphorylation events in the vanadium-induced respiratory burst of alveolar macrophages. Toxicol. Appl. Pharmacol. 156, 170-178 (1999).
40. H. Wang and J. A. Joseph, Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic. Biol. Med. 27, 612-616 (1999).
41. J. K. Leach, G. Van Tuyle, P. S. Lin, R. Schmidt-Ullrich and R. B. Mikkelsen, Ionizing radiation-induced, mitochondria-dependent generation of reactive oxygen/nitrogen. Cancer Res. 61, 3894-3901 (2001).
42. J. K. Leach, S. M. Black, R. K. Schmidt-Ullrich and R. B. Mikkelsen, Activation of constitutive nitric-oxide synthase activity is an early signaling event induced by ionizing radiation. J. Biol. Chem. 277, 15400-15406 (2002).
43. H. D. Soule, J. Vazguez, A. Long, S. Albert and M. Brennan, A human cell line from a pleural effusion derived from a breast carcinoma. J. Natl. Cancer Inst. 51, 1409-1416 (1973).
44. S. P. Goss, R. J. Singh and B. Kalyanaraman, Bicarbonate enhances the peroxidase activity of Cu, Zn-superoxide dismutase. Role of carbonate anion radical. J. Biol. Chem. 274, 28233-28239 (1999).
45. H. Zhang, J. Joseph, M. Gurney, D. Becker and B. Kalyanaraman, Bicarbonate enhances peroxidase activity of Cu, Zn-superoxide dismutase. Role of carbonate anion radical and scavenging of carbonate anion radical by metalloporphyrin antioxidant enzyme mimetics. J. Biol. Chem. 277, 1013-1020 (2002).
46. H. Zhang, J. Joseph, C. Felix and B. Kalyanaraman, Bicarbonate enhances the hydroxylation, nitration, and peroxidation reactions catalyzed by copper, zinc superoxide dismutase. Intermediacy of carbonate anion radical. J. Biol. Chem. 275, 14038-14045 (2000).
47. G. Rothe and G. Valet, Flow cytometric analysis of respiratory burst activity in phagocytes with hydroethidine and 2′,7′ -dichlorofluorescin. J. Leukoc. Biol. 47, 440-448 (1990).
48. R. A. Wolber, R. E. Duque, J. P. Robinson and H. A. Oberman, Oxidative product formation in irradiated neutrophils. A flow cytometric analysis. Transfusion 27, 167-170 (1987).
49. C. Rota, Y. C. Fann and R. P. Mason, Phenoxyl free radical formation during the oxidation of the fluorescent dye 2′,7′ -dichlorofluorescein by horseradish peroxidase. Possible consequences for oxidative stress measurements. J. Biol. Chem. 274, 28161-28168 (1999).
50. C. Rota, C. F. Chignell and R. P. Mason, Evidence for free radical formation during the oxidation of 2′-7′-dichlorofluorescin to the fluorescent dye 2′-7′-dichlorofluorescein by horseradish peroxidase: possible implications for oxidative stress measurements. Free Radic. Biol. Med. 27, 873-881 (1999).
51. H. Zhu, G. L. Bannenberg, P. Moldeus and H. G. Shertzer, Oxidation pathways for the intracellular probe 2′,7′-dichlorofluorescein. Arch. Toxicol. 68, 582-587 (1994).
52. L. N. Larsen, E. Dahl and J. Bremer, Peroxidative oxidation of leucodichlorofluorescein by prostaglandin H synthase in prostaglandin biosynthesis from polyunsaturated fatty acids. Biochim. Biophys. Acta 1299, 47-53 (1996).
53. E. Marchesi, C. Rota, Y. C. Fann, C. F. Chignell and R. P. Mason, Photoreduction of the fluorescent dye 2′-7′-dichlorofluorescein: A spin trapping and direct electron spin resonance study with implications for oxidative stress measurements. Free Radic. Biol. Med. 26, 148-161 (1999).