Development of a transformation and selection system for the glycolipid-producing yeast Candida bombicola

Yeast

Volumn 25, Issue 4, 2008, Pages 273-278

  Van Bogaert, Inge N A ^a   De Maeseneire, Sofie L ^a   Develter, Dirk ^b   Soetaert, Wim ^a   Vandamme, Erick J ^a  

^a GHENT UNIVERSITY   (Belgium)

^b Ecover Belgium NV ^*  (Belgium)

Author keywords

Candida bombicola; Genetic transformation; Glycolipids; Orotidine 5 phosphate decarboxylase; Sophorolipids; URA3

Indexed keywords

GLYCOLIPID; OROTIDINE 5' PHOSPHATE DECARBOXYLASE; URACIL;

ARTICLE; AUXOTROPHIC MUTANT; CANDIDA; CONTROLLED STUDY; ENZYME SYNTHESIS; FUNGAL STRAIN; FUNGUS CULTURE; GENETIC SELECTION; GENETIC TRANSFORMATION; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; WILD TYPE;

AMINO ACID SEQUENCE; CANDIDA; DNA, FUNGAL; FUNGAL PROTEINS; GENETIC ENGINEERING; GLYCOLIPIDS; MOLECULAR SEQUENCE DATA; MUTATION; OROTIDINE-5'-PHOSPHATE DECARBOXYLASE; SEQUENCE ALIGNMENT; TRANSFORMATION, GENETIC; URACIL;

STARMERELLA BOMBICOLA;

EID: 42549134963     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.1586     Document Type: Article

References (14)

1. Alani E, Cao L, Kleckner N. 1987. A method for gene disruption that allows repeated use of ufa3 selection in the construction of multiply disrupted yeast strains. Genetics 116: 541-545.
2. Brachmann CB, Davies A, Cost GJ, et al. 1998. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132.
3. Boeke JD, Lacroute F, Fink GR. 1984. A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast 5-fluoro-orotic acid resistance. Mol Gen Genet 197: 345-346.
4. Francois F, Chapeland-Leclerc F, Villard J, Noel T. 2004. Development of an integrative transformation system for the opportunistic pathogenic yeast Candida lusitaniae using URA3 as a selection marker. Yeast 21: 95-106.
5. Gietz RD, Schiestl RH. 1995. Transforming yeast with DNA. Methods Mol Cell Biol 5: 255-269.
6. Goosen T, Bloemheuvel G, Gysler C, et al. 1987. Transformation of Aspergillus niger using the homologous orotidine-5′-phosphate-decarboxylase gene. Curr Genet 11: 499-503.
7. Kimsey HH, Kaiser D. 1992. The orotidine-5′-monophosphate decarboxylase gene of Myxococcus xanthus; comparison to the OMP decarboxylase gene family. J Biol Chem 267: 819-824.
8. Rodriguez L, Chavez FP, Gonzalez ME, et al. 1998. Isolation and sequence analysis of the orotidine-5′-phosphate decarboxylase gene (URA3) of Candida utilis. Comparison with the OMP decarboxylase gene family. Yeast 14: 1399-1406.
9. Rosa CA, Lachance MA. 1998. The yeast genus Starmerella gen. nov. and Starmerella bombicola sp. nov., the teleomorph of Candida bombicola (Spencer, Gorin & Tullock) Meyer & Yarrow. Int J Syst Bacteriol 48: 1413-1417.
10. Rose M, Grisaf P, Botstein D. 1984. Structure and function of the yeast URA3 gene expression in Escherichia coli. Gene 29: 113-124.
11. Sakai Y, Kazarimoto T, Tani Y. 1991. Transformation system for an asporogenous methylotrophic yeast, Candida boidinii; cloning of the orotidine-5′-phosphate decarboxylase gene (ura3), isolation of uracil auxotrophic mutants, and use of the mutants for integrative transformation. J Bacteriol 173: 7458-7463.
12. Van Bogaert INA, De Maeseneire SL, De Schamphelaire W, et al. 2007a. Cloning, characterization and functionality of the orotidine-5′-phosphate decarboxylase gene (URA3) of the glycolipid-producing yeast Candida bombicola. Yeast 24: 201-208.
13. Van Bogaert INA, Saerens K, De Muynck C, et al. 2007b. Microbial production and application of sophorolipids. Appl Microbiol Biotechnol 76: 23-34.
14. Yang VW, Marks JA, Davis BP, Jeffries TW. 1994. High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2. Appl Environ Microbiol 60: 4245-4254.