Development of an integrative transformation system for the opportunistic pathogenic yeast Candida lusitaniae using URA3 as a selection marker

Yeast

Volumn 21, Issue 2, 2004, Pages 95-106

  François, Fabienne ^a   Chapeland Leclerc, Florence ^a   Villard, Jean ^a   Noël, Thierry ^a,b  

^a UNIVERSITÉ PARIS DESCARTES   (France)

^b Faculté de Pharmacie ^*  (France)

Author keywords

Candida lusitaniae; GEA2; Genetic transformation; Homologous recombination; Orotidine 5 phosphate decarboxylase; URA3

Indexed keywords

BIOLOGICAL MARKER; DNA; FLUOROOROTIC ACID; FUNGAL PROTEIN; MUTANT PROTEIN; OROTIDINE 5' PHOSPHATE DECARBOXYLASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BIOTRANSFORMATION; CANDIDA LUSITANIAE; CHROMOSOME WALKING; CONTROLLED STUDY; ELECTROPORATION; FUNGAL STRAIN; FUNGUS MUTANT; GENE IDENTIFICATION; GENE LOCUS; GENE VECTOR; HOMOLOGOUS RECOMBINATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SACCHAROMYCES; SEQUENCE HOMOLOGY; SOUTHERN BLOTTING; YEAST;

AMINO ACID SEQUENCE; BASE SEQUENCE; BLOTTING, SOUTHERN; CANDIDA; CLONING, MOLECULAR; CONSERVED SEQUENCE; DNA, FUNGAL; FUNGAL PROTEINS; GUANINE NUCLEOTIDE EXCHANGE FACTORS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; PHYLOGENY; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ALIGNMENT; TRANSFORMATION, GENETIC;

CANDIDA; CLAVISPORA LUSITANIAE; SACCHAROMYCETALES;

EID: 1242296315     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.1059     Document Type: Article

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