Lack of αvβ5 integrin receptor or its ligand MFG-E8: Distinct effects on retinal function

Ophthalmic Research

Volumn 40, Issue 3-4, 2008, Pages 120-123

  Nandrot, Emeline F ^a   Finnemann, Silvia C ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

Blindness; Integrin; Ligand; Phagocytosis; Photoreceptors; Retinal pigment epithelium

Indexed keywords

ALPHAVBETA5 INTEGRIN; INTEGRIN RECEPTOR; LACTADHERIN; PROTEIN TYROSINE KINASE; BIOLOGICAL MARKER; CCL2 PROTEIN, MOUSE; CCR2 PROTEIN, MOUSE; CHEMOKINE RECEPTOR; CHEMOKINE RECEPTOR CCR2; CX3CR1 PROTEIN, MOUSE; CYTOKINE; INTEGRIN; INTEGRIN ALPHAVBETA8; LIGAND; MEMBRANE ANTIGEN; MFGE8 PROTEIN, MOUSE; MILK PROTEIN; MONOCYTE CHEMOTACTIC PROTEIN 1;

AGED; AGING; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BLINDNESS; CIRCADIAN RHYTHM; CONTROLLED STUDY; ELECTRORETINOGRAM; MOUSE; NONHUMAN; PHAGOCYTOSIS; PHOTORECEPTOR; PIGMENT EPITHELIUM; PRIORITY JOURNAL; PROTEIN DEFICIENCY; PROTEIN FUNCTION; PROTEIN SECRETION; RETINA; VISION; WILD TYPE; ANIMAL; C57BL MOUSE; COMPARATIVE STUDY; DISEASE MODEL; FLUORESCENCE MICROSCOPY; GENETICS; METABOLISM; MOUSE MUTANT; PATHOLOGY; RETINA MACULA DEGENERATION; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

ANIMALS; ANTIGENS, SURFACE; BIOLOGICAL MARKERS; CHEMOKINE CCL2; CYTOKINES; DISEASE MODELS, ANIMAL; INTEGRINS; LIGANDS; MACULAR DEGENERATION; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; MICROSCOPY, ELECTRON, TRANSMISSION; MICROSCOPY, FLUORESCENCE; MILK PROTEINS; PIGMENT EPITHELIUM OF EYE; RECEPTORS, CCR2; RECEPTORS, CHEMOKINE;

EID: 42449159819     PISSN: 00303747     EISSN: None     Source Type: Journal    
DOI: 10.1159/000119861     Document Type: Article

References (18)

1. Young RW: The renewal of photoreceptor cell outer segments. J Cell Biol 1967;33:61-72.
2. Young RW, Bok D: Participation of the retinal pigment epithelium in the rod outer segment renewal process. J Cell Biol 1969;42:392-403.
3. Goldman AI, Tierstein PS, O'Brien PJ: The role of ambient lighting in circadian disc shedding in the rod outer segment of the rat retina. Invest Ophthalmol Vis Sci 1980;19:1257-1267.
4. LaVail MM: Rod outer segment disk shedding in rat retina: relationship to cyclic lighting. Science 1976;194:1071-1074.
5. Mullen RJ, LaVail MM: Inherited retinal dystrophy: primary defect in pigment epithelium determined with experimental rat chimeras. Science 1976;192:799-801.
6. Rakoczy PE, Zhang D, Robertson T, Barnett NL, Papadimitriou J, Constable IJ, Lai CM: Progressive age-related changes similar to age-related macular degeneration in a transgenic mouse model. Am J Pathol 2002;161:1515-1524.
7. Finnemann SC, Leung LW, Rodriguez-Boulan E: The lipofuscin component A2E selectively inhibits phagolysosomal degradation of photoreceptor phospholipid by the retinal pigment epithelium. Proc Natl Acad Sci USA 2002;99:3842-3847.
8. Lakkaraju A, Finnemann SC, Rodriguez-Boulan E: The lipofuscin fluorophore A2E perturbs cholesterol metabolism in retinal pigment epithelial cells. Proc Natl Acad Sci USA 2007;104:11026-11031.
9. Sparrow JR, Boulton M: RPE lipofuscin and its role in retinal pathobiology. Exp Eye Res 2005;80:595-606.
10. D'Cruz PM, Yasumura D, Weir J, Matthes MT, Abderrahim H, LaVail MM, Vollrath D: Mutation of the receptor tyrosine kinase gene Mertk in the retinal dystrophic RCS rat. Hum Mol Genet 2000;9:645-651.
11. Nandrot E, Dufour EM, Provost AC, Pequignot MO, Bonnel S, Gogat K, Marchant D, Rouillac C, Sepulchre de Conde B, Bihoreau MT, Shaver C, Dufier JL, Marsac C, Lathrop M, Menasche M, Abitbol MM: Homozygous deletion in the coding sequence of the c-mer gene in RCS rats unravels general mechanisms of physiological cell adhesion and apoptosis. Neurobiol Dis 2000;7:586-599.
12. Edwards RB, Szamier RB: Defective phagocytosis of isolated rod outer segments by RCS rat retinal pigment epithelium in culture. Science 1977;197:1001-1003.
13. Nandrot EF, Kim Y, Brodie SE, Huang X, Sheppard D, Finnemann SC: Loss of synchronized retinal phagocytosis and age-related blindness in mice lacking αvβ5 integrin. J Exp Med 2004;200:1539-1545.
14. Nandrot EF, Anand M, Almeida D, Atabai K, Sheppard D, Finnemann SC: Essential role for MFG-E8 as ligand for αvβ5 integrin in diurnal retinal phagocytosis. Proc Natl Acad Sci USA 2007;104:12005-12010.
15. Finnemann SC, Bonilha VL, Marmorstein AD, Rodriguez-Boulan E: Phagocytosis of rod outer segments by retinal pigment epithelial cells requires αvβ5 integrin for binding but not for internalization. Proc Natl Acad Sci USA 1997;94:12932-12937.
16. Hanayama R, Tanaka M, Miwa K, Nagata S: Identification of a factor that links apoptotic cells to phagocytes. Nature 2002;417:182-187.
17. Finnemann SC, Rodriguez-Boulan E: Macrophage and retinal pigment epithelium phagocytosis: apoptotic cells and photoreceptors compete for αvβ3 and αvβ5 integrins, and protein kinase C regulates αvβ5 binding and cytoskeletal linkage. J Exp Med 1999;190:861-874.
18. Nandrot EF, Anand M, Sircar M, Finnemann SC: Novel role for αvβ5 integrin in retinal adhesion and its diurnal peak. Am J Physiol Cell Physiol 2006;290:C1256-C1262.