Ficoll-separated mononuclear cells from sepsis patients are contaminated with granulocytes

Intensive Care Medicine

Volumn 34, Issue 5, 2008, Pages 912-916

  Van Den Akker, Erica L T ^a   Baan, Carla C ^a   Van Den Berg, Bart ^a   Russcher, Henk ^a   Joosten, Koen ^a   Hokken Koelega, Anita C S ^a   Lamberts, Steven W J ^a   Koper, Jan W ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

Ficoll; Granulocytes; Mononuclear cells; Sepsis

Indexed keywords

FICOLL;

AGED; ARTICLE; CASE REPORT; CELL FRACTIONATION; CONTAMINATION; CONTROLLED STUDY; FLOW CYTOMETRY; GRANULOCYTE; HUMAN; IMMUNOFLUORESCENCE; INTENSIVE CARE; MALE; MONONUCLEAR CELL; NEUTROPHIL; PROSPECTIVE STUDY; SCHOOL CHILD; SEPARATION TECHNIQUE; SEPSIS;

AGED; CASE-CONTROL STUDIES; CELL SEPARATION; CENTRIFUGATION, DENSITY GRADIENT; CHILD; FICOLL; GRANULOCYTES; HUMANS; LEUKOCYTES, MONONUCLEAR; MALE; MONITORING, IMMUNOLOGIC; REPRODUCIBILITY OF RESULTS; SEPSIS;

EID: 42449143428     PISSN: 03424642     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00134-007-0989-0     Document Type: Article

References (19)

1. Annane D, Bellissant E, Cavaillon JM (2005) Septic shock. Lancet 365:63-78
2. Boyum A (1976) Isolation of lymphocytes, granulocytes and macrophages. Scand J Immunol Suppl 5:9-15
3. Adib-Conquy M, Adrie C, Moine P, Asehnoune K, Fitting C, Pinsky MR, Dhainaut JF, Cavaillon JM (2000) NF-kappaB expression in mononuclear cells of patients with sepsis resembles that observed in lipopolysaccharide tolerance. Am J Respir Crit Care Med 162:1877-1883
4. Molijn GJ, Spek JJ, van Uffelen JC, de Jong FH, Brinkmann AO, Bruining HA, Lamberts SW, Koper JW (1995) Differential adaptation of glucocorticoid sensitivity of peripheral blood mononuclear leukocytes in patients with sepsis or septic shock. J Clin Endocrinol Metab 80:1799-1803
5. Nakamori Y, Ogura H, Koh T, Fujita K, Tanaka H, Sumi Y, Hosotsubo H, Yoshiya K, Irisawa T, Kuwagata Y, Shimazu T, Sugimoto H (2005) The balance between expression of intranuclear NF-kappaB and glucocorticoid receptor in polymorphonuclear leukocytes in SIRS patients. J Trauma 59:308-314; discussion 314-315
6. Papathanassoglou ED, Moynihan JA, McDermott MP, Ackerman MH (2001) Expression of Fas (CD95) and Fas ligand on peripheral blood mononuclear cells in critical illness and association with multiorgan dysfunction severity and survival. Crit Care Med 29:709-718
7. Stanilova SA, Karakolev ZT, Dimov GS, Dobreva ZG, Miteva LD, Slavov ES, Stefanov CS, Stanilov NS (2005) High interleukin 12 and low interleukin 10 production after in vitro stimulation detected in sepsis survivors. Intensive Care Med 31:401-407
8. van Leeuwen HJ, van der Bruggen T, van Asbeck BS, Boereboom FT (2001) Effect of corticosteroids on nuclear factor-kappaB activation and hemodynamics in late septic shock. Crit Care Med 29:1074-1077
9. Levy MM, Fink MP, Marshall JC, Abraham E, Angus D, Cook D, Cohen J, Opal SM, Vincent JL, Ramsay G (2003) 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference. Intensive Care Med 29:530-538
10. Goldstein B, Giroir B, Randolph A (2005) International Pediatric Sepsis Consensus Conference: definitions for sepsis and organ dysfunction in pediatrics. Pediatr Crit Care Med 6:2-8
11. Knaus WA, Draper EA, Wagner DP, Zimmerman JE (1985) APACHE II: a severity of disease classification system. Crit Care Med 13:818-829
12. Pollack MM, Ruttimann UE, Getson PR (1988) Pediatric risk of mortality (PRISM) score. Crit Care Med 16:1110-1116
13. Helleman P (ed) (1980) Hematologie, 3rd edn. Elsevier, Amsterdam
14. Summerfield A, Hofmann MA, McCullough KC (1998) Low density blood granulocytic cells induced during classical swine fever are targets for virus infection. Vet Immunol Immunopathol 63:289-301
15. Calvano SE, Greenlee PG, Reid AM, deRiesthal HF, Shires GT, Antonacci AC (1988) Granulocyte contamination of Ficoll-Hypaque preparations of mononuclear cells following thermal injury may lead to substantial overestimation of lymphocyte recovery. J Trauma 28:353-361
16. Deitch EA, Xu D, Sitting K, Wohlman M, Bridges RM, Landry K, McDonald JC (1989) Ficoll-Hypaque leukocyte preparations from burned patients contain activated nonlymphoid cell populations that take up thymidine. J Trauma 29:277-283
17. Volenec FJ, Wood GW, Mani MM, Robinson DW, Humphrey LJ (1979) Mononuclear cell analysis of peripheral blood from burn patients. J Trauma 19:86-93
18. Heidecke CD, Hensler T, Weighardt H, Zantl N, Wagner H, Siewert JR, Holzmann B (1999) Selective defects of T lymphocyte function in patients with lethal intraabdominal infection. Am J Surg 178:288-292
19. Fujimoto H, Sakata T, Hamaguchi Y, Shiga S, Tohyama K, Ichiyama S, Wang F, Houwen B (2000) Flow cytometric method for enumeration and classification of reactive immature granulocyte populations. Cytometry 42:371-378