How fast can TCSPC FLIM be made?

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 6771, Issue , 2007, Pages

  Katsoulidou, Vicky ^a   Bergmann, Axel ^a   Becker, Wolfgang ^a  

^a BECKER AND HICKL GMBH   (Germany)

Author keywords

Chlorophyll transients; FLIM; Fluorescence; TCSPC

Indexed keywords

CELLS; CHLOROPHYLL; FLUOROPHORES; PIXELS; PLANTS (BOTANY); TISSUE;

CHLOROPHYLL TRANSIENTS; FLUORESCENCE LIFETIME IMAGES; PLANT TISSUE;

IMAGE ANALYSIS;

EID: 42449128010     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.735550     Document Type: Conference Paper

References (12)

1. R.M. Ballew, J.N. Demas, An error analysis of the rapid lifetime determination method for the evaluation of single exponential decays, Anal. Chem. 61, 30 (1989)
2. W. Becker, H. Hickl, C. Zander, K.H. Drexhage, M. Sauer, S. Siebert, J. Wolfrum, Time-resolved detection and identification of single analyte molecules in microcapillaries by time-correlated single photon counting, Rev. Sci. Instrum. 70, 1835-1841 (1999)
3. W. Becker, A. Bergmann, G. Biscotti, K. Koenig, I. Riemann, L. Kelbauskas, C. Biskup, High-speed FLIM data acquisition by time-correlated single photon counting, Proc. SPIE 5323, 27-35 (2004)
4. W. Becker, A. Bergmann, M.A. Hink, K. König, K. Benndorf, C. Biskup, Fluorescence lifetime imaging by time-correlated single photon counting, Micr. Res. Techn. 63, 58-66 (2004)
5. W. Becker, Advanced time-correlated single-photon counting techniques. Springer, Berlin, Heidelberg, New York, 2005
6. W. Becker, The bh TCSPC handbook, second edition. Becker & Hickl GmbH (2006), available on www.becker-hickl.com
7. W. Becker, A. Bergmann, C. Biskup, Multi-Spectral Fluorescence Lifetime Imaging by TCSPC, Micr. Res. Tech. 70, 403-409 (2007)
8. Becker & Hickl GmbH, DCS-120 confocal scanning FLIM systems, user handbook. Available on www.becker-hickl.com
9. H.C. Gerritsen, M.A.H. Asselbergs, A.V. Agronskaia, W.G.J.H.M. van Sark, Fluorescence lifetime imaging in scanning microscopes: acquisition speed, photon economy and lifetime resolution, J. Microsc. 206, 218-224 (2002)
10. Govindjee, Sixty-three Years Since Kautsky: Chlorophyll a Fluorescence, Aust. J. Plant Physiol. 22, 131-160 (1995)
11. M. Köllner, J. Wolfrum, How many photons are necessary for fluorescence-lifetime measurements?, Phys. Chem. Lett. 200, 199-204 (1992)
12. D. V. O'Connor, D. Phillips, Time-correlated single photon counting, Academic Press, London (1984)