Probing of cardiomyocyte metabolism by spectrally resolved lifetime detection of NAD(P)H fluorescence

Computers in Cardiology

Volumn 34, Issue , 2007, Pages 349-352

  Aneba, S ^a   Cheng, Y ^a   Mateasik, A ^b   Comte, B ^a,c   Chorvat Jr , D ^b   Chorvatova, A ^a,d  

^a UNIVERSITY OF MONTREAL   (Canada)

^b INTERNATIONAL LASER CENTER   (Slovakia)

^c UNIVERSITY OF MONTREAL   (Canada)

^d UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOFLUORESCENCE; CARDIAC CELLS; CARDIOMYOCYTE; EFFECTIVE MANAGEMENTS; ELECTRON DONORS; ENZYMATIC REACTIONS; FLUORESCENCE LIFE-TIME; FLUORESCENCE SPECTRUM; GLUTATHIONE REDUCTASE; IN CELLS; IN-VITRO; LASER DIODES; LIPOAMIDE DEHYDROGENASE; NAD(P)H; OXIDATIVE METABOLISMS; PICOSECOND; TIME-CORRELATED SINGLE-PHOTON COUNTING; UV LIGHTS;

BIOCHEMISTRY; CARDIOLOGY; LASER EXCITATION; METABOLISM; PULSED LASER APPLICATIONS;

FLUORESCENCE;

EID: 42149168912     PISSN: 02766574     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1109/CIC.2007.4745493     Document Type: Conference Paper

References (4)

1. +-isocitrate dehydrogenase activity and expression: a marker of oxidative stress in hypertrophy development. Am J Physiol Heart Circ Physiol 2004;287:H2122-H2131.
2. Blinova K, Carroll S, Bose S et al. Distribution of mitochondrial NADH fluorescence lifetimes: steady- state kinetics of matrix NADH interactions. Biochemistry 2005;44:2585-2594.
3. +/NADH binding and the structural basis of disease-causing mutations. J Mol Biol 2005;350:543-552.
4. Chorvat D Jr, Chorvatova A. Spectrally resolved time- correlated single photon counting: a novel approach for characterization of endogenous fluorescence in isolated cardiac myocytes. Eur Biophys J 2006;36:73-83.