The effect of flocculation on the efficiency of raw-starch fermentation by Saccharomyces cerevisiae producing the Lipomyces kononenkoae LKA1-encoded α-amylase

Annals of Microbiology

Volumn 58, Issue 1, 2008, Pages 99-108

  Ramachandran, Nivetha ^a,c   Joubert, Lydia ^b   Gundlapalli, Sarath B ^a,d   Cordero Otero, Ricardo R ^a,e   Pretorius, Isak S ^a,f  

^a Institute for Wine Biotechnology

^b STELLENBOSCH UNIVERSITY   (South Africa)

^c HOSPITAL FOR SICK CHILDREN RESEARCH INSTITUTE   (Canada)

^d YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e UNIVERSITAT ROVIRA I VIRGILI   (Spain)

^f AUSTRALIAN WINE RESEARCH INSTITUTE   (Australia)

Author keywords

Bioethanol; Flocculation; Lipomyces kononenkoae; Saccharomyces cerevisiae; Starch fermentation; Yeast; amylase

Indexed keywords

LIPOMYCES KONONENKOAE; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 42149147496     PISSN: 15904261     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF03179452     Document Type: Article

References (33)

1. Atia K.S., Ismail S.A., El-Arnaouty M.B., Dessouki A.M. (2003). Use of co-immobilized beta-amylase and pullulanase in reduction of saccharification time of starch and increase in maltose yield. Biotechnol. Prog., 19: 853-857.
2. Ausubel P.M., Brent R., Kingston R.E., Eds (1994). Current Protocols in Molecular Biology, vol. 13.0.1-13.14.17, Wiley, New York.
3. Birol G., Onsan I., Kidar B., Oliver S.G. (1998). Ethanol production and fermentation characteristics of recombinant Saccharomyces cerevisiae strains grown on starch. Enzyme. Microb. Technol., 22: 672-677.
4. Eksteen J.M., van Rensberg P., Cordero Otero R.R., Pretorius I.S. (2003). Starch fermentation by recombinant Saccharomyces cerevisiae strains expressing the α-amylase and glucoamylase genes from Lipomyces kononenkaoe and Saccharomycopsis fibuligera. Biotechnol. Bioeng., 84: 639-646.
5. Gagiano M., Bauer F.F., Pretorius I.S. (2002). The sensing of nutritional status and the relationship to filamentous growth in Saccharomyces cerevisiae. FEMS Yeast Res., 2: 433-470.
6. Gietz D., St. Jean A., Woods R.A., Schiestl R.H. (1992). Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res., 20: 1425.
7. Immel S., Lichtenthaler F.W. (2000). The hydrophobia topographies of amylose and its blue iodine complex. Starch - Stärke, 52: 1-8.
8. Janse B.J.H., Pretorius I.S. (1995). One step enzymatic hydrolysis of starch by a recombinant strain of Saccharomyces cerevisiae encoding α-amylase, glucoamylase and pullulanase. Appl. Microbiol. Biotechnol., 42: 878-883.
9. Khaw T.S., Katakura Y., Koh J., Kondo A., Ueda M., Shioya S (2006). Evaluation of performance of different surface-engineered yeast strains for direct ethanol production from raw starch. Appl. Microbiol. Biotechnol. 70: 573-579.
10. Kobayashi O., Suda H., Ohtani T., Sone H. (1996). Molecular cloning and analysis of the dominant flocculation gene FLO8 from Saccharomyces cerevisiae. Mol. Gen. Genet., 251: 707-715.
11. Kondo A., Shigechi H., Abe M., Uyama K., Matsumoto T., Takahashi S., Ueda M., Tanaka A., Kishimoto M., Fukuda H. (2002). High-level ethanol production from starch by a flocculent Saccharomyces cerevisiae strain displaying cell-surface glucoamylase. Appl. Microbiol. Biotechnol., 58: 291-296.
12. Liu H., Styles C.A., Fink G.R. (1996). Saccharomyces cerevisiae S288C has a mutation in FLO8, a gene required for filamentous growth. Genetics, 144: 967-978.
13. Liu Y., Hama H., Fujita Y., Kondo A., Inoue Y., Kimura A., Fukuda H. (1998). Production of S-lactoglutathione by high activity whole cell biocatalysts prepared by permeabilization of recombinant Saccharomyces cerevisiae with alcohols. Biotechnol. Bioeng., 64: 54-60.
14. Lynd L.R., Weimer P.J., van Zyl W.H., Pretorius I.S. (2002). Microbial cellulose utilization: fundamentals and biotechnology. Microbiol. Mol. Biol. Rev., 66: 506-577.
15. Madhani H.D., Fink G.R. (1998). The control of filamentous differentiation and virulence in fungi. Trends Cell. Biol., 8: 348-353.
16. Masy C.L., Henquinet A., Mestdagh M.M. (1992). Flocculation of Saccharomyces cerevisiae: inhibition by sugars. Can. J. Microbiol., 38: 1298-306.
17. Mizoguchi H., Hara S. (1996). Effect of fatty acid saturation in membrane lipid bilayers on simple diffusion in the presence of ethanol at high concentrations. J. Ferment. Bioeng., 81: 406-411.
18. Morris V.J., Gunning A.P., Faulds C.B., Williamson G., Svensson B. (2005). AFM images of complexes between amylose and Aspergillus niger glucoamylase mutants, native and mutant starch binding domains: a model for the action of glucoamylase. Starch-Stärke, 57: 1-7.
19. Murai T., Ueda M., Yamamura M., Atomi H., Shibasaki Y., Kamasawa N., Osumi M., Amachi T., Tanaka A. (1997). Construction of starch-utilizing yeast by cell surface engineering. Appl. Environ. Microbiol., 63: 1362-1366.
20. Murai T., Ueda M., Shibasaki Y., Kamasawa N., Osumi M., Imanaka T., Tanaka A. (1999). Development of an arming yeast strain for efficient utilization of starch by co-display of sequential amylolytic enzymes on the cell surface. Appl. Microbiol. Biotechnol., 51: 65-70.
21. Nakamura Y., Kobayashi F., Ohnaga M., Sawada T. (1997). Alcohol fermentation of starch by genetic recombinant yeast having glucoamylase activity. Biotechnol. Bioeng., 53: 21-25.
22. Powell C. D., Quain D.E., Smart K.A. (2003). The impact of brewing yeast cell age on fermentation performance, attenuation and flocculation. FEMS Yeast Res., 3: 149-57.
23. Pretorius I.S. (1997). Utilization of polysaccharides by Saccharomyces cereviase. In: Zimmermann FK, Entian K.D., Eds, Yeast Sugar Metabolism, Technomic Publishing Company, Lancaster, pp. 435-458.
24. Rodriguez E., Callieri D.A.S. (1983). Conversion of sucrose to ethanol by a flocculent Zymomonas sp. isolated from sugar cane juice. Biotechnol. Lett., 8: 744-748.
25. Seong K.T., Katakura Y., Ninomiya K., Bito Y., Katahira S., Kondo A., Ueda M., Shioya S. (2006). Effect of flocculation on performance of arming yeast in direct ethanol fermentation. Appl. Microbiol. Biotechnol. 73: 60-66.
26. Shigechi H., Uyama K., Fujita Y., Matsumoto T., Ueda M., Tanaka A., Fukuda H., Kondo A. (2002). Efficient ethanol production from starch through development of novel flocculent yeast strains displaying glucoamylase and co-displaying or secreting α-amylase. J. Mol. Catalysis B: Enzymatic, 17: 179-187.
27. Shigechi H., Koh J., Fujita Y., Matsumoto T., Bito Y., Ueda M., Satoh E., Fukuda H., Kondo A. (2004). Direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and α-amylase. Appl. Environ. Microbiol., 70: 5037-5040.
28. Smit G., Straver M.H., Lugtenberrg B.J.J., Kijne J.W. (1992). Flocculence of Saccharomyces cerevisiae cells is induced by nutrient limitation, with cell surface hydrophobicity as a major determinant. Appl. Environ. Microbiol., 58: 3709-3714.
29. Sreenath H.K., Koegel R.G., Holdes A.B., Jeffries T.W., Straub R.J. (2001). Ethanol production from alfalfa fiber fractions by saccharification and fermentation. Proc. Biochem., 36: 1199-1204.
30. Steyn A.J.C., Pretorius I.S. (1991). Co-expression of a Saccharomyces diastaticus glucoamylase-encoding gene and a Bacillus amyloliquefaciens α-amylase-encoding gene in Saccharomyces cerevisiae. Gene, 100: 85-93.
31. Steyn A.J.C., Marmur J., Pretorius I.S. (1995). Cloning, sequence analysis and expression in yeasts of a cDNA containing a Lipomyces kononenkoae α-amylase-encoding gene. Gene, 166: 65-71.
32. Steyn A.J.C., Pretorius I.S. (1995). Characterization of a novel α-amylase from Lipomyces kononenkoae and expression of its gene (LKA1) in Saccharomyces cerevisiae. Curr. Genet., 28: 526-533.
33. Winston F., Dollard C., Ricupero Hovasse S.L. (1995). Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C. Yeast, 11: 53-55.