Comparison of protein quantification and extraction methods suitable for E. coli cultures

Biologicals

Volumn 36, Issue 3, 2008, Pages 198-202

  De Mey, Marjan ^a   Lequeux, Gaspard J ^a   Maertens, Jo ^a   De Muynck, Cassandra I ^a   Soetaert, Wim K ^a   Vandamme, Erick J ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Escherichia coli; Metabolic modeling; Protein extraction; Protein quantification

Indexed keywords

ARTICLE; BACTERIUM CULTURE; COMPARATIVE STUDY; ESCHERICHIA COLI; EXTRACTION; MEASUREMENT; METABOLIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXTRACTION; RELIABILITY;

BIOCHEMISTRY; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CHLOROFORM; ESCHERICHIA COLI; HYDROXIDES; METABOLISM; MICROBIOLOGICAL TECHNIQUES; MODELS, STATISTICAL; MODELS, THEORETICAL; POTASSIUM COMPOUNDS; PROTEINS; QUINOLINES; SODIUM DODECYL SULFATE; SPECTROPHOTOMETRY, ULTRAVIOLET; TOLUENE;

ESCHERICHIA COLI;

EID: 41949114072     PISSN: 10451056     EISSN: 10958320     Source Type: Journal    
DOI: 10.1016/j.biologicals.2007.10.001     Document Type: Article

References (12)

1. Bailey J.E., Sburlati A.R., Hatzimanikatis V., Lee K., Renner W.A., and Tsai P.S. Inverse metabolic engineering: a strategy for directed genetic engineering of useful phenotypes. Biotechnol Bioeng 52 (1996) 109-121
2. De Mey M., Lequeux G.J., Maertens J., De Maeseneire S.L., Soetaert W., and Vandamme E.J. Comparison of DNA and RNA quantification methods suitable for parameter estimation in metabolic modeling of microorganisms. Anal Biochem 353 (2006) 198-203
3. De Mey M., Lequeux G.J., Beauprez J.J., Maertens J., Van Horen E., Soetaert W.K., et al. Comparison of different strategies to reduce acetate formation in Escherichia coli. Biotechnol Prog 23 (2007) 1053-1063
4. Deutscher M.P. Guide to protein purification (1990), Academic Press Inc., San Diego, California
5. Miller J.H. Experiments in molecular genetics (1972), Cold Spring Harbor Press, Cold Spring Harbor, NY
6. Verduyn C., Postma E., Scheffers W.A., and Vandijken J.P. Physiology of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat Cultures. J Gen Microbiol 136 (1990) 395-403
7. Sambrook J., and Russell D.W. Molecular cloning: a laboratory manual. 3rd ed. (2001), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
8. Ohnishi S.T., and Barr J.K. A simplified method of quantitating protein using the biuret and phenol reagents. Anal Biochem 86 (1978) 193-200
9. Schultze U. Anaerobic physiology of Saccharomyces cerevisiae (1995), Technical University of Denmark, Copenhagen
10. Neter J., Kutner M.H., Nachtsheim C.J., and Wasserman W. Applied linear statistical models. 4th ed. (1996), McGraw-Hill, Boston
11. Gentleman R., and Ihaka R. The R project for statistical computing (1997), R Foundation for Statistical Computing.
12. Carlson R., and Srienc F. Fundamental Escherichia coli biochemical pathways for biomass and energy production: identification of reactions. Biotechnol Bioeng 85 (2004) 1-13