Validation of the IDI-MRSA™ system for use on pooled nose, axilla, and groin swabs and single swabs from other screening sites

Diagnostic Microbiology and Infectious Disease

Volumn 61, Issue 1, 2008, Pages 1-5

  Jeyaratnam, Dakshika ^a,b   Gottlieb, Anna ^a   Ajoku, Uchechukwu ^a   French, Gary L ^a,b  

^a GUY'S AND ST THOMAS' NHS FOUNDATION TRUST   (United Kingdom)

^b KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

IDI MRSA; MRSA; Nonnasal; Pooling; Screening

Indexed keywords

ARTICLE; AXILLA; CULTURE MEDIUM; GEL; HEATING; HUMAN; INGUINAL REGION; INTERMETHOD COMPARISON; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; NOSE SMEAR; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PUNCTURE; RECTUM; SCREENING; SENSITIVITY AND SPECIFICITY; THROAT CULTURE; VALIDATION PROCESS; WOUND;

AXILLA; BACTERIOLOGICAL TECHNIQUES; CARRIER STATE; DNA, BACTERIAL; GROIN; HUMANS; METHICILLIN RESISTANCE; NOSE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SKIN; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS;

EID: 41949085096     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2007.12.004     Document Type: Article

References (18)

1. BD GeneOhm™ MRSA Test Product Insert.
2. Bishop E., Grabsch E.A., Ballard S.A., Mayall B., Xie S., Martin R., and Grayson M.L. Concurrent analysis of nose and groin swab specimens by the IDI-MRSA PCR assay is comparable to analysis by individual specimen PCR and routine culture assays for detection of colonization by methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 44 (2006) 2904-2908
3. Brown D.F.J., Edwards D.I., Hawkey P.M., Morrison D., Ridgway G.L., Towner K.J., and Wren W.D. On behalf of the joint working party of the British society for antimicrobial chemotherapy, hospital infection society and infection control nurses association. Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA). J. Antimicrob. Chemother. 56 (2005) 1000-1018
4. Chambers H.F. Methicillin resistance in staphylococci: molecular implications and biochemical basis and clinical implications. Clin. Microbiol. Rev. 10 (1997) 781-791
5. Coello R., Jimenez J., Garcia M., Arroyo P., Minguez D., Fernández C., Cruzet F., and Gaspar C. Prospective study of infection, colonisation and carriage of methicillin-resistant Staphylococcus aureus in an outbreak affecting 990 patients. Eur. J. Clin. Microbiol. Infect. Dis. 13 (1994) 74-81
6. Coia J.E., Duckworth G.J., Edwards D.I., Farrington M., Fry C., Humphreys H., Mallaghan C., Tucker D.R., for the Joint Working Party of the British Society of Antimicrobial Chemotherapy, the Hospital Infection Society, and and the Infection Control Nurses Association. Guidelines for the control and prevention of methicillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities. J. Hosp. Infect. 63 Suppl 1 (2006) S1-S44
7. Cunningham R., Jenks P., Northwood J., Wallis M., Ferguson S., and Hunt S. Effect on MRSA transmission of rapid PCR testing of patients admitted to critical care. J. Hosp. Infect. 65 (2007) 24-28
8. De San N., Denis O., Gasasira M.-F., De Mendonça R., Nonhoff C., and Struelens M.J. Controlled evaluation of the IDI-MRSA assay for the detection of colonization by methicillin-resistant Staphylococcus aureus (MRSA) in diverse muco-cutaneous specimens. J. Clin. Microbiol. 45 (2007) 1098-1101
9. Desjardins M., Guibord C., Lalonde B., Toye B., and Ramotar K. Evaluation of the IDI-MRSA assay for detection of methicillin-resistant Staphylococcus aureus from nasal and rectal specimens pooled in a selective broth. J. Clin. Microbiol. 44 (2006) 1219-1223
10. Drews S.J., Willey B.M., Kreiswith N., Wang M., Ianes T., Mitchell J., Latchford M., McGeer A.J., and Katz K.C. Verification of the IDI-MRSA assay for detecting methicillin-resistant Staphylococcus aureus in diverse specimen types in a core clinical laboratory setting. J. Clin. Microbiol. 44 (2006) 3794-3796
11. Gurran C., Holliday M.G., Perry J.D., Ford M., Morgan S., and Orr K.E. A novel selective medium for the detection of methicillin-resistant Staphylococcus aureus enabling result reporting in under 24 h. J. Hosp. Infect. 52 (2002) 148-151
12. Harbarth S., Masuet-Aumatell C., Schrenzel J., Francois P., Akakpo C., Renzi G., Pugin J., Ricou B., and Pittet D. Evaluation of rapid screening and pre-emptive contact isolation for detecting and controlling methicillin-resistant Staphylococcus aureus in critical care: an interventional cohort study. Crit. Care. Med. 10 (2006) R25
13. Huletsky A., Giroux R., Rossbach V., Gagnon M., Vaillancourt M., Bernier M., Gagnon F., Truchon K., Bastien M., Picard F.J., Van Belkum A., Ouellette M., Roy P.H., and Bergeron M.G. New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J. Clin. Microbiol. 42 (2004) 1875-1884
14. Kelly S., Hardwick R., Wong J., and Rao G.G. Laboratory evaluation of selective mannitol broth for MRSA screening. J. Hosp. Infect. 58 (2004) 239-240
15. Reyes R, Catanzariti E, Isaac E, John M, Stoakes L, Vandierendonck J, Hussain Z (2006) Comparison of a real-time PCR assay and a chromogenic MRSA selective medium for the detection of methicillin resistant Staphylococcus aureus directly from nasal and rectal swabs. Clin Microbiol Infect 12(Suppl 4):1-1 [P950].
16. Struelens M.J. Rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) and patient management. Clin. Microbiol. Infect. 12 Suppl 9 (2006) 23-26
17. Warren D.K., Liao R.S., Merz L.R., and Eveland M. Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay. J. Clin. Microbiol. 42 (2004) 5578-5581
18. Zhang X.S., Drews S.J., Tomassi J., and Katz K.C. Comparison of two versions of the IDI-MRSA assay using charcoal swabs for prospective nasal and nonnasal surveillance samples. J. Clin. Microbiol. 45 (2007) 2278-2280