Quantitative analysis of Epstein-Barr virus supernatant by real-time PCR assay

Cell Preservation Technology

Volumn 6, Issue 1, 2008, Pages 73-81

  Fecenko Tacka, Karen L ^a   Schina, Laura ^a   Beiswanger, Christine M ^a  

^a Laboratory of Molecular and Cellular Biology of Hematopoiesis   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS DNA;

ARTICLE; B LYMPHOCYTE; CELL GROWTH; CELL LINE; CELL TRANSFORMATION; CRYOPRESERVATION; DILUTION; DNA DETERMINATION; EPSTEIN BARR VIRUS; LYMPHOBLASTOID CELL; LYMPHOBLASTOID CELL LINE; LYMPHOCYTE; MEASUREMENT; NONHUMAN; PRIORITY JOURNAL; PROTEOMICS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SUPERNATANT; VIRION; VIRUS TITRATION;

HUMAN HERPESVIRUS 4;

EID: 41649108629     PISSN: 1538344X     EISSN: None     Source Type: Journal    
DOI: 10.1089/cpt.2007.0515     Document Type: Article

References (18)

1. Steinberg K, Beck J, Nickerson D, et al. DNA banking for epidemiologic studies: A review of current practices. Epidemiology 2002;13:246-254.
2. Beck JC, Beiswanger CM, John EM, et al. Successful transformation of cryopreserved lymphocytes: A resource for epidemiological studies. Cancer Epidemiol Biomarkers Prev 2001;10:551-554.
3. Bernacki SH, Beck JC, Muralidharan K, et al. Characterization of publically available lymphoblastoid cell lines for disease-associated mutations in 11 genes. Clin Chem 2005;51:2156-2159.
4. Bernacki SH, Beck JC, Stankovic AK, et al. Genetically characterized positive control cell lines derived from residual clinical blood samples. Clin Chem 2005;51:2013-2024.
5. Bernacki SH, Stankovic AK, Williams LO, et al. Establishment of stably EBV-transformed cell lines from residual clinical blood samples for use in performance evaluation and quality assurance in molecular genetic testing. J Mol Diag 2003;5:227-231.
6. Bolton BJ, Spurr NK. B-lymphocytes. In: Freshney RI, Freshney MG, ed. Culture of Immortalized Cells. New York: Wiley-Liss, Inc.; 1996:283-297.
7. Fung HC, Scholz S, Matarin M, et al. Genome-wide genotyping in Parkinson's disease and neurologically normal controls: First stage analysis and public release of data. Lancet Neurol 2006;5:911-916.
8. Keller MA, Gwinn K, Nash J, et al. Whole genome association studies of neurophsychiatric disease: An emerging era of collaborative genetic discovery. Neuropsy Dis Treat 2007;3:613-618.
9. Gwinn K, Corriveau RA, Mitsumoto H, et al., Amyotrophic lateral sclerosis: An emerging era of collaborative gene discovery. PloS One 2007;2:e1254.
10. Atwal GS, Bond GL, Metsyuanum S, et al. Haplotype structure and selection of the MDM2 oncogene in humans. Proc Natl Acad Sci USA 2007;104:4524-4529.
11. Andrulionyte L, Peltola P, Chiasson JL, et al. Single nucleotide polymorphisms of PPARD in combination with the Gly482Ser substitution of PGC-1A and the Pro12Ala substitution of PPARG2 predict the conversion from impaired glucose tolerance to type 2 diabetes: The STOP-NIDDM trial. Diabetes 2006;55:2148-2152.
12. Benusiglio PR, Pharoah PD, Smith PL, et al. HapMap-based study of the 17q21 ERBB2 amplicon in susceptibility to breast cancer. Br J Cancer 2006;95:1689-1695.
13. Kimura H, Morita M, Yabuta Y, et al. Quantitative analysis of Epstein-Barr virus load by using a realtime PCR assay. J Clin Microbiol 1999;37:132-136.
14. Niesters HGM, Van Esser J, Fries E, et al. Development of a real-time quantitative assay for detection of Epstein-Barr virus. J Clin Microbiol 2000;38:712-715.
15. Wadosky RM, Laus S, Green M, et al. Measurement of Epstein-Barr virus DNA loads in whole blood and plasma by TaqMan PCR and in peripheral blood lymphocytes by competitive PCR. J Clin Microbiol 2003;41:5245-5249.
16. Nonoyama M, Pagano JS. Detection of Epstein-Barr viral genome in non-productive cells. Nat New Biol 1997;233:103-106.
17. Shannon-Lowe C, Baldwin G, Feederle R, et al. Epstein-Barr virus induced B-cell transformation: Quantitating events from virus binding to cell outgrowth. J Gen Virol 2005;86:3009-3019.
18. Hitchman RB, Siaterli EA, Nixon CP, et al. Quantitative real-time PCR for rapid and accurate titration of recombinant baculovirus particles. Biotechnol Bioeng 2006;96:810-814.